PhD student in Genetics here. You have mastered the art of scientific story telling here! This style is like a warm personalized version of deep look or science Friday with experimental play by play. This format has legs for mass appeal I think ❤ great job! Liked and subscribed. Should you decide to I could see you with a blooming future in science communications 🙂
This is so exciting! I'm applying to PhD's right now after wanting to work with transgenic plants since I was 15 years old. I hope I get to use this protocol in my research, because that's really cool.
Love to see other plant genetics labs as a current undergrad in an arabidopsis epidermal cell fate lab. Really cool vid and it’s so cool to see the methods I’m using be used for a completely different project! Also I’m so glad that I can do agro dips with arabidopsis, but moments like this are so amazing!
Very cool video. The only thing that caught my attention was that I did not hear any mention of using an antibiotic resistance gene for the cali selection (like hygromycin resistance). Just using a visual marker like GFP is fine but you can generate cali with mixed cell populations so there could be some Wt cells in with the transformed cells. Is there a reason for doing it that way?
So, when you plant these genetically modified plants or regenerate them, the GFP marker inserted into their genetic code will be retained. As the plants grow and produce seeds, the GFP trait will be passed down to the next generation. This means that when you receive seeds from the new plants, their growth cycle will also express the GFP trait, and they will retain the glowing characteristics of the original plants. Is this correct?
@@markmatzke5836 yes!! So much more efficient than VIGS. VIGS plants couldn't pass the genetic transformation on to their offspring, so we had to repeat the experiment every time we needed more plants
I'm really curious about those developmental genes that were added to the plasmid, but I couldn't find the article that talks about it in Yan's research. Can anyone give me a pointer so I can find more information about it?
So the callus culture phase is only to select transformed cells into plants? If you treat the plants with antibiotics after the vacuum step, do they still die or do the apical meristems remain unchanged? Or what is the problem?
During transformation events only a low percentage of cells will actually be transformed, and even then many of the transformations are unstable and will get removed from the plant’s genome after a few days. The cali stage is there to give the few transformed cells time to grow and regenerate into a whole plant. You would need to be extremely lucky or just transform thousands of plants if you wanted to get the meristem transformed. Making a Cali from every cell of the plant ensures you get something out of it
@@justsomeguy4935 Yes, this was my suspicion. It's just that example A. thaliana can be transformed with floral dip method alone. Of course not all seeds are transformed, but enough still. No need for callus culture.
Have you referenced Dr. Michael Levin's work on ions? Non-nurial intelligence and agential materials? It's looking at the DNA a misleading path? Dr Levin's work indicated the controlling factor seems to be via ionic electrical stimulation.. or the software. Not the DNA hardware. The atoms ionic properties in the proteins generated by the DNA not the DNA itself?
PhD student in Genetics here. You have mastered the art of scientific story telling here! This style is like a warm personalized version of deep look or science Friday with experimental play by play.
This format has legs for mass appeal I think ❤ great job! Liked and subscribed.
Should you decide to I could see you with a blooming future in science communications 🙂
@@offmeds2niteaw shucks thanks so much 🥹
This is so exciting! I'm applying to PhD's right now after wanting to work with transgenic plants since I was 15 years old. I hope I get to use this protocol in my research, because that's really cool.
It was superb to follow through this !! Molly and Yan please make glowing flowers with from these dev regulatory genes.
So cool to see the plant side of science
Got recommended this video, lots of effort in the editing, congrats on your work, great video!
MOLLY this is so cool!!! Omg I'm so happy for you & all your plant friends 🌸
Thank you 😭the science is gonna be NEXT LEVEL now that we have this protocol
Great job, Molly and Yan
Thank you Cankui! With Plantago and Aquilegia transformation protocols there is going to be such amazing new science happening 🤗
So glad the YT algorithm is getting better again. This was a great video!
im just comming out of college and its so nice seeing people who absouletly love thier respective fields
Love to see other plant genetics labs as a current undergrad in an arabidopsis epidermal cell fate lab. Really cool vid and it’s so cool to see the methods I’m using be used for a completely different project!
Also I’m so glad that I can do agro dips with arabidopsis, but moments like this are so amazing!
@@Awesome-oz4dh I will forever be jealous of Arabidopsis dips 😂
Woah so cool to see plant side of science!!! Biology could be cool too, grateful to see this vid❤
really cool! 4:53 does the new transformation protocol still retain the ability to turn genes off?
Yes! We were adding fun glowy genes in this case, but you could totally use it to turn genes off instead
Very cool video. The only thing that caught my attention was that I did not hear any mention of using an antibiotic resistance gene for the cali selection (like hygromycin resistance). Just using a visual marker like GFP is fine but you can generate cali with mixed cell populations so there could be some Wt cells in with the transformed cells. Is there a reason for doing it that way?
Awesome, do you know what the developmental genes added to help the plant reform?
Wox5!
@ScienceIRL thank you!
So, when you plant these genetically modified plants or regenerate them, the GFP marker inserted into their genetic code will be retained. As the plants grow and produce seeds, the GFP trait will be passed down to the next generation. This means that when you receive seeds from the new plants, their growth cycle will also express the GFP trait, and they will retain the glowing characteristics of the original plants. Is this correct?
@@markmatzke5836 yes!! So much more efficient than VIGS. VIGS plants couldn't pass the genetic transformation on to their offspring, so we had to repeat the experiment every time we needed more plants
I'm really curious about those developmental genes that were added to the plasmid, but I couldn't find the article that talks about it in Yan's research. Can anyone give me a pointer so I can find more information about it?
@@Kemecgabriel he used WOX5! 🧬🤗
yan CAN cook!
So the callus culture phase is only to select transformed cells into plants?
If you treat the plants with antibiotics after the vacuum step, do they still die or do the apical meristems remain unchanged? Or what is the problem?
During transformation events only a low percentage of cells will actually be transformed, and even then many of the transformations are unstable and will get removed from the plant’s genome after a few days. The cali stage is there to give the few transformed cells time to grow and regenerate into a whole plant. You would need to be extremely lucky or just transform thousands of plants if you wanted to get the meristem transformed. Making a Cali from every cell of the plant ensures you get something out of it
@@justsomeguy4935 Yes, this was my suspicion. It's just that example A. thaliana can be transformed with floral dip method alone. Of course not all seeds are transformed, but enough still. No need for callus culture.
💖💖💖💖
cool beans
Have you referenced Dr. Michael Levin's work on ions? Non-nurial intelligence and agential materials? It's looking at the DNA a misleading path? Dr Levin's work indicated the controlling factor seems to be via ionic electrical stimulation.. or the software. Not the DNA hardware.
The atoms ionic properties in the proteins generated by the DNA not the DNA itself?