What is Gel Electrophoresis | Don't Memorise
HTML-код
- Опубликовано: 30 июл 2024
- 🎯NEET 2024 Paper Solutions with NEET Answer Key: • NEET Answer Key 2024 |...
📅🆓NEET Rank & College Predictor 2024: infinitylearn.com/neet-rank-p... How do we separate the desired DNA fragment from a complete mixture of several DNA fragments? The job sounds almost impossible! But there is a technique that comes to our rescue! Gel Electrophoresis is a separation technique which separates DNA fragments based on their Length or Size. Want to know how the technique works? Watch this video to understand the Principle and Technique of Gel Electrophoresis.
In this video, we will learn:
0:00 Introduction
0:44 definition of Gel Electrophoresis
1:19 is DNA charged?
2:20 principle and working of Gel Electrophoresis
2:48 principle of separation of the DNA fragments
3:57 requirements for Gel Electrophoresis
3:55 Gel Electrophoresis process
6:24 DNA separation
7:25 Elution
Don’t Memorise brings learning to life through its captivating educational videos. To Know More, visit InfinityLearn.com
New videos every week. To stay updated, subscribe to our RUclips channel : bit.ly/DontMemoriseRUclips
NCERT Solutions for Class 6 to 12 - Free CBSE NCERT Solutions
infinitylearn.com/surge/study...
Register on our website to gain access to all videos and quizzes:
infinitylearn.com/microcourse...
Connect with us!
Instagram page: idontmemorise?i...
Facebook page: DontMemorise...
#GelElectrophoresis #AgaroseGel #Electrophoresis #neet2024 #infinityLearnNEET #neetsyllabus #neet2025
This is by far the best explaination of gel electrophoresis I've come across. thanks a million
Hey ! how do we separate cloning vector and recognition site that we cut from vector for introducing desired gene
My question is are cloning vector also extracted through gel electrophoresis
@@Mahimahi-rn2ol what is a cloning vector from your point of view?
Me too
Agree!
I can't thank you enough for such a clear explanation of this. You literally helped relieve my last minute practical exam preparation stress. Thank you so much.
Hey , I'm really thankful to you , because u r really helping students in understanding things, and I know there is lot of hardwork behind these animated vedios.God bless you🤲🙏🏻
Thank you so much Angel for your appreciation. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. We love our Pi Army and would like to stay connected. 🤗🤗
malayaali daaaaa
Neet
The beauty of this channel is that they answer literally every question that will arise in your head about the topic ❤️
Thank you so much for teaching this in such an easy and understanding way!!
Best explanation for gel electrophoresis I've come across. simple and to the point explanation of the topic . Thank u a lot for this...❤️
This is the only video you will need on this topic
Gel Electrophoresis was a puzzle for me until I found your video.. Thank you.. For such a great animation.. ❤
Raymundo, Francezka J.
•Gel Electrophoresis is a separation technique which separates DNA fragments based on their Length or Size.
•The movement of charged molecules is exposed in electrical field and it occurs in gel medium, that's why it's called Gel electrophoresis.
•To observe the DNA molecule, agarose gel is used with ethidium bromide (ethidium bromide is used beacuse when the agarose gel containing the DNA is observed under ultraviolet light, bright color bands are clearly seen).
•Gel electrophoresis is considered to be one of the important techniques in recombinant DNA tech.
God bless you mam
Salting, Colleen L.
8-Avogadro
-The separation of DNA fragments based on their sizes is called Gel Electrophoresis
-Technique is based on the movement of charged molecules occurring in an electrical field; occurs in the Gel Medium
-The DNA is negatively charged due to Phosphate groups
-The DNA fragments may contain DNA fragments. Because of different sizes those which are smaller move ahead, and those larger in size move slower
-The gel has a mesh-like structure, similar to a sieve
Gel Electrophoresis:
-Since the DNA is negatively charged it will move towards the positively charged electrode
-Using a colored loading dye can help identify and track the DNA in the gel since they are invisible
DNA separation:
-Ethidium Bromide easily binds to the DNA molecules; this helps us see bright-colored bands under UV light
-To know the size of the DNA bands a DNA Ladder is used
-Elution is the manual extraction of a DNA fragment from the Agarose Gel used
-Gel Electrophoresis is considered one of the most important techniques in recombinant DNA technology
naknam, hi cols
Tiongco, Gabriel Felix D.
8-Edison
Gel Electrophoresis
• It is considered as one of the most important techniques in rDNA Technology.
• It is a separation technique which separates DNA fragments based on their length or size.
• It is based on the movement of charged molecules when exposed to an electrical field (occurs in a gel medium).
• DNA is negatively charged. It is due to phosphate groups which are present in the backbone of the DNA's double helix.
• The gel generally used for DNA is agarose gel which are obtained from seaweeds.
• Treating ethidium bromide to the agarose gel makes it possible to see the separated DNA molecules because ethidium bromide easily binds to the DNA molecules.
• The DNA ladder is a standard chart used to compare the bands obtained to easily identify the desired DNA. After obtaining the desired DNA, we can cut and extract the DNA fragment from the agarose gel which is a process called elution.
Thank you so much for this video. It's crystal clear now
Kudos ..this is just so to the point ..I am brushing my Biotech syllabus , that I have studied 8 years back ..and this helped me really
Thank you so much🙏
Best explanation till date I got for one of the most important molecular biology techniques -Gel electrophoresis.
Superb.Thank you for such an amazing lecture
Rebote, Brendan Liam P.
8-Avogadro
Gel Electrophoresis
• used to separate the different DNA fragments based on their sizes
• movements of charged molecules when exposed in an electrical field and this movement occurs in an gel medium, hence gel electrophoresis.
Process
• DNA negatively charged (Due to phosphate groups)
• Phosphate groups are present in the backbone of the double helix.
• Agarose gel is usually used for DNA
• The charge across the gel helps the mobility of the DNA fragments.
• Not all fragments move at the same pace. (smaller fragments move ahead, bigger fragments find it difficult to move)
Procedure
• Requirements for gel electrophoresis
- DNA sample
- Gel
- Casting tray
- Comb
- Electrical Supply
• The wells are formed from the negative charge so that the DNA will go from negative charge to positive charge.
• A colour loaded dye is used to track the movement of the DNA samples.
•Buffer is used for better conductivity of electricity.
• The loaded dye is much faster than the DNA samples because it must reach the anode first. If the loaded dye reaches the anode, it must mean that the DNA samples reached somewhere near.
• To observe the DNA samples, we treat the agarose gel with ethidium bromide solution.
• Ethitidum bromide is used because it easily bonds with the DNA molecules
• DNA bonds can be seen under a UV light
• DNA ladder is used to compare the size
• Cutting and extraction of DNA fragment from the agarose gel is called Elution.
•
Thanks so much for dropping a detailed video, really helpful it's a great one🤗
oh my gosh. this channel is literally my new go to for genetics, I love it
Thanks for the detailed video. This is very informative and useful.
the most easy and best explanation i have ever heard in my whole eduactional exposure love u maam
Very thoroughly explained. Thanks!
Thank you so much.. for simple, nice & clear explaining 🎀
Very helpful. Thankyou,keep up the good work.
Excellent video. Well explained touching all concept in and out of Gel electrophoresis
This made all the things clear so simply!!!
Ya.
You prolly dont care at all but does anybody know of a tool to get back into an instagram account..?
I somehow forgot my login password. I would love any assistance you can give me.
@Rayan Garrett Instablaster =)
@Payton Ignacio Thanks for your reply. I got to the site thru google and Im trying it out atm.
Looks like it's gonna take a while so I will get back to you later when my account password hopefully is recovered.
@Payton Ignacio it worked and I now got access to my account again. I am so happy!
Thank you so much you saved my account :D
You helped me much in physics to understand different topics and you have helped me in bio also. YOU ARE ONE OF MY BEST TEACHERS. GOD BLESS YOU ALWAYS...😍😍
Wow wow wow. That's all i can say. Bravo!
It is simply amazing....
Hats off to your hardwork👏👏💯
Thank you so much...Cleared all doubts 👍👍
thank you very much for the best explanation of gel electrophoresis
Thank you so much! It's easy to understand😊
This is so well explained thanks
amazing , its as clear as water and easy to understand
Thank you so much
I m really..happy...coz i searched the whole internet...but couldnt find the video with this kind of explanation...
Ik few points r also there which r not mentioned here...but overall the video is the best...100/100
Thank you so much for crystal clear explanation
your explanation is incredible, thanks a bunch! it's useful for me
Congrats on 1.68 Million subs!!
The explanation is very easy to understand u have done the good thing to teach the students who really want thanks so much video is very clear and easy thnx again ☺️
It's my pleasure. We are glad that you understood the concept. We are happy now that you are all clear about your doubts. You motivate us to do better. Keep watching our videos : )
The best lectures over electrophoresis 👍🏻👍🏻👍🏻
This is awesome. i definetely love this explanation.
Extraordinary explaination
Thank you for sharing very informative video ❤️
Thank you so much!!🥰
Angelica May D. Salvatierra : Bohr
- Gel electrophoresis is a technique for separating DNA fragments depending on various sizes.
- It is called "Gel Electrophoresis" because it is based on a movement of charged molecules in an electrical field that occurs in a gel medium.
-The process of Gel Electrophoresis needs tools such as a casting tray, gel, comb, electrical supply, and DNA Sample (Different sized DNA Fragments).
-The DNA molecules are seen using an agarose gel with ethidium bromide (quickly attaches to DNA molecules). Light brilliant color bands can be seen when the agarose gel containing the DNA is examined under ultraviolet light.
- The size or length of a DNA fragment can be determined by comparing the bands obtained to the DNA ladders (standard chart).
Crystal clear....explanation is on point
YOU ARE MY BEST TEACHER!
This was amazing! Thank you
It's my pleasure. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. Keep watching our videos : )
Ur way of explanation is really amazing
It's excellent....
Clearly explained hidden Words... Like Agarose which is given from Seed...
Or DNA got Position charge because of phosphate ❤️❤️❤️❤️
Love From Pakistan 🇵🇰
Pakistan Zindabad!
Well explained!!
The best YT channel!
Well explained
Zalamea, Mary Joyce H.
8 - Edison
Gel Electrophoresis
- Gel electrophoresis is a method used for separating DNA fragments based on their sizes using Agarose gel, a gel that can be obtained from seaweeds.
- A DNA molecule is negatively charged due to the phosphate groups found in its double-helix structure.
- A colored loading dye is added to the mixture to easily track the DNA due to it being colorless.
- DNA samples are loaded near the negative electrode side of the gel as they move towards the positive electrode using the electric current.
- Agarose gel is treated with Ethidium Bromide solution, as Ethidium Bromide can easily bind to the DNA molecules.
- Elution is the process of cutting and extracting DNA fragments from Agarose Gel.
ge
ty so much for such an amazing explanation
Most welcome, Shyny!
Keep watching! 🙂🙂
a lot of question are clear that have in my mind i am really thankful......................
Best explanation I have ever seen
Thank you so much.. Nice explanation
Perfect Explanation
Perfecttttt thank u so much ❤️❤️
Absolutely love the graphics...
Thanks for Explaining😊
Very helpful video!
Superb explanation 👌👌👌
Best explanation ever in my life
Fernandez, Akiko B. | 8-Chatelet
- Gel electrophoresis is the separation of DNA fragments on a piece of gel. It is based on the movement of charged molecules when expose to the electric field. It was said that Gel electrophoresis is considered a very important technique used in recombinant DNA.
- The charge present on the DNA molecule is negatively charged due to phosphate groups.
- Agarose gel is the gel used for Gel electrophoresis and it is obtained from seaweeds.
- It is said that the smaller fragments move faster than the large fragments, and they move from negative to positive (they can track the DNA moving by using the colored Dye).
- To easily bind the DNA molecule, treat the agarose gel with ethidium bromide solution.
- DNA ladders will help us know the size of DNA fragments.
- Elution is the cutting and extraction of DNA fragments from Agarose gel.
- The extraction of DNA can be used for further downstream processing.
Thank u mam🤗, u make it easy for me 🥰
Great work and Awesome ANimation
Just PERFECT ' Perfecttt Lecture '' Thankyou so much ♥️♥️
You're most welcome. We are glad that you understood the concept. We are glad that you understood the concept. You motivate us to do better. Keep watching our videos : )
@@InfinityLearn_NEET tq
Thanks for this best explanation
Best best explanation..thank you
Very nicely explained
No words to say this explanation is amazing which is very clearly understandable to students from non biology background also👌👌
Thanks a ton Lalitha for your appreciation. We are glad to hear that it was helpful for you. We hope that you understood the concept. We hope now you are clear your all doubts. Keep watching. 😊😊 😊😊
@@InfinityLearn_NEET Iam already subscribed ur channel mam plz do more videos on Molecular biology and biophysics techniques
@@InfinityLearn_NEET mam but may be small size particles get struck into the pores of gel how can they move faster and why large molecules move slow please do explain.......
Ever The best video on this topic 🤗
Thanks 😊
helped a lot! thank you so much :)
You're most welcome. We are really happy to hear that it was helpful to you. We are glad that you understood the concept. You motivate us to do better. Keep watching our videos. 😊😊
Great explanation :)
One of the best👍💯
Nice teaching.. 😍well understood👍
Thank you so much.
My beloved youtube chanel
You're most welcome. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. Keep watching our videos. 😊😊
Well explained.
It's very useful
Very nice explanation
Thank you 😃
It stucks in my mind tq 👍
nice explanation...good work.
Outstanding mam
Your the best teacher maam
Waiting for the next video
Superb explanation
Thank you
IMPRESSIVE CONCEPT
The quality of the video is excellent.... thank u so much............😍😍😍🥰🥰
You're most welcome Aakash. We are glad that you understood the concept. We are happy that we could help you learn. You motivate us to do better. Keep watching our videos : )
@@InfinityLearn_NEET You r welcome Mam ...
I have a request can u make the videos of biotechnology (full chapter) in this manner so that we can understand it in more better way and will get help for the upcoming juniors.......
thank you
best explanation..
fantastic
Best explanation.. 👍🏻👍🏻
great video thank you
You're most welcome Arman and Thanks for your appreciation. We are glad that you understood the concept. You motivate us to do better. Keep watching our videos. 😊😊
best and best explanation
Beautifully Explained Ma'am.. Thank you
You're most welcome and Thank you so much for your appreciation. We are glad that you understood the concept. Do support us by subscribing to our channel. 👍👍
Perfect