Hi! Since you didn't get any response, I thought I could offer my 2 cents on the matter. If you transport the water sample whole it would be suggested to transport it in ice (≥4C). However this possibility might be unrealistic in field studies. That's why they use a preservative. I don't know what the "preservative" they mention is, but I've read that Longmire's lysis buffer can help to keep the filtered eDNA stable at room temperature for a few months even, and other more homemade solutions like ethanol and sodium acetate can preserve the eDNA for about a week, also at room temperature. Furthermore, if you've managed to establish some other protocol with good results I'd love to hear of it!
Yeah, thanks alot for the safety and clothing advice, I really needed that
Hi! After having the filtered sample with DNAiso Reagent, does it needs to be placed in a certain temperature during transport? Thanks!
Hi! Since you didn't get any response, I thought I could offer my 2 cents on the matter. If you transport the water sample whole it would be suggested to transport it in ice (≥4C). However this possibility might be unrealistic in field studies. That's why they use a preservative. I don't know what the "preservative" they mention is, but I've read that Longmire's lysis buffer can help to keep the filtered eDNA stable at room temperature for a few months even, and other more homemade solutions like ethanol and sodium acetate can preserve the eDNA for about a week, also at room temperature. Furthermore, if you've managed to establish some other protocol with good results I'd love to hear of it!