Choosing a mounting medium for microscopy
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- Опубликовано: 30 сен 2024
- Today I will make permanent microscope slides using different mounting media. Let's see how they compare.
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I use a formula for pollen mounts, it is a combination of glycerin, gelatin and Listerine mouthwash (prevents mold in the jelly). I melt on the slide with a warming table, after adding the pollen and cover slip, the gelatin becomes semi-sold.
Many, many thanks for all of your efforts this past year - entertainment with information! Have a good rest over Christmas! Best wishes to you & yours!
Oliver, you have inspired me in many ways re: microscopy and this time you energized me to create a DIY slide ringing device. I used 2 CD disks and mounted on a large bolt and inserted into a hand drill to make it spin. Works great. I tried uploading a photo to the forum but was unable to post the photos.
Hi there,
May I ask where you got your slide spinner from, the electric ones are quite pricey.
Also, when using nail varnish as a mounting agent, can you mount a coverslip over a dry specimen using the circle technique? Or do you still need to add something on top of specimen.
Slide ringing table (scroll down): www.brunelmicroscopessecure.co.uk/acatalog/universal.html
You can make a "dry mount" without mounting medium over the specimen. But you still need to make a circle first (1) on the blank slide, then you add the specimen, then the cover glass, and then you make another circle (2) to glue the cover glass on circle (1). If you do not make circle (1) first, then the nail polish will go under the cover glass when you put on circle (2). If circle (1) is too dry then the cover glass will not hold when you put it on. in this case add another circle over (1) to make the cover glass stick.
@@Microbehunter Thank you!
@Microbehunter, the video is hidden, it doesn't show up in the channel, I don't know if you have hidden it on purpose.
Hello, the video will be online in a few hours. RUclips is still processing it.
i have a question for knowledgeable people, if somebody could please help me.
i love been in the woods and i have a couple of Sawyer water filters. i recently saw a rare video of somebody testing a couple different water filter model and according to his results many were clearly defective. i am shocked and i am searching around for an easy way to test those.
somebody has an easy~ way to test potable water for bacteria? ive seen tests for sale online, but according to the reviews they dont seem reliable and a waste of money. should i just buy a microscope and "manually" check the water sample by eye? maybe try the petri dish method and make cultures?
A microscope is not going to help. Bacteria may be present but at a too low concentration to see them. You would have to culture them to do a colony count. Normally these tests should have been done by the company who sell the filters. Also the presence of bacteria alone is not the issue. It depends on the type of bacteria present (ie fecal bacteria) and different kinds can not be distinguished with a microscope anyway.
@@Microbehunter i see. Thank you very much for your expertise. i was right to consult we a knowledgeable person before. you saved my alot of time and money with your advice.
i wish you Happy Christmas!