I really miss those old long videos where you give a loooot of details about everything and show us every step you take, from collecting the specimen to observing it
I have tried the same. I collected some pond water and found some sticks in my garden that looked nearly the same as in your video, lots of lichen on it and rather decomposed. With the water and the wood chips I added a small piece of oat in a petri dish. I scanned a slide every day at 200x magnification twice (once with phase contrast and once with normal lighting). After four days the amount of bacteria had increased enormously with many different types of bacteria (rod shaped that looked like elongated colonies of small round bacteria, spiral shaped bacteria, and larger balls that seemed to small to be ciliates. Only very occasionally I saw single celled organisms that were not bacteria (a lone vorticella and something that resembled a very small paramecium). I also left a stick in a jar with a shallow amount of pond water. From that stick I inspected some removed material every day. I found no amoeba as of yet. Is it possible that the area where you collected your wood stick is particularly abundant with amoeba? Or did you collect your stick from a pond?
I have three questions, Oliver. 1. Would old newspaper instead of tissue do if cut to fit the petri dish and wetted? 2. Should the petri dish be placed by a window for light or in a dark place, or does it not matter? 3. When will your first newsletter be issued?
1. No. Not newspaper. The ink and solvent in the newspaper might do harm. Or allow the newspaper to air out a bit. It the newspaper s smelly (petrol?) then it's not good. As a matter of fact I remember reading somewhere that new newspaper should be used to roll in carpets for storage, as it keeps insects etc away. 2. Light irrelevant, becasue no algae that grow (which would need light). These are heterotrophs, decomposers that feed on organic material and this is their food source. 3. I am almost finished. Maybe in the next few days.
That's really interesting about the newspaper and carpets to keep insects away. I wondered about newspaper because I used to keep a wormery and the earth worms thrived on torn up dampened newspaper. Thanks, Oliver.
@@Microbehunter: I did the experiment exactly as you said except I did use newspaper. After three days in the petri dish, I made a slide up just as you did with a drop of water and a cover glass on top. I found lots of microbes, but, unfortunately, none large enough at 400x to see any great detail. There was one microbe that appeared in and out of the image at great speed and was too fast to focus on and capture with the camera. I didn't get anything of the quality of image that you got, though, but interesting nonetheless. I'm trying again, but this time I am using a piece of wetted cardboard cut out of a cardboard egg carton (I am a stickler for recycling stuff), some rotten wood bark, and a single oat for food.
Hi am can you please respond to my question, is it safe to grow bacteria or fungus at home and how long can you allow them to grow after they need to be disposed please ?
It all depends on the type. If you make your own yogurt, then you are growing your own bacteria, and this is generally safe. With fungi, it is not healthy to inhale the spores, but also here the type of fungus is relevant. For this reason, one should not grow unknown micororganisms. And even in this video here I have grown unknown microbes and one should be careful. But every time when food spoils, you are growing unknown bacteria and fungi. So I would say, do not grow microorgnisms that you isolated from your own body (eg.skin).
@@Microbehunter okay thanks I also love your videos I am a biology a d chemistry student and I'm studying to later on becoming a surgeon and I get inspired by your videos I recently bought a compound microscope from your advice
Could someone explain me in spanish what to do to grow the ameba, please? This is the best video that I'd seen about it, but I don't understsnd what is he saying because he's speaking very fast and my English isn't very good. Thanks!! You can write in english too and I'll translate.
I realize this is a 3 year old post I’m responding to, but I’ll add a comment anyway. I’ve experimented with taking a piece of thread, coating it with a small amount of Vaseline, laying the thread in a circle on the slide, putting the drop of water with your microbes in the center of the circle, and putting the cover slip on top of that. This will allow you to keep the sample longer. The trade-off is that the deeper sample means that critters can swim in and out of focus more easily.
not recommended. you risk that the objective touches the water. Or you allow water to evaporate, ciliate then dies and does not move. You can then observe at high magnification.
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@@afxgrin i hope you’re joking
The amoeba on the thumbnail looked like a horse and cool
I really miss those old long videos where you give a loooot of details about everything and show us every step you take, from collecting the specimen to observing it
You mean those where I started in nature collecting and then moving into the lab?
@@Microbehunter I second this!
Thank you for your videos! I´m IT student but I like watching your videos cause a lot of informations are there.
Cool vid again, thx microbe hunter!! Keep up the good work mate!
this helped me find tons of amoebs thanks also please make more videos and amoeba
I have tried the same. I collected some pond water and found some sticks in my garden that looked nearly the same as in your video, lots of lichen on it and rather decomposed. With the water and the wood chips I added a small piece of oat in a petri dish. I scanned a slide every day at 200x magnification twice (once with phase contrast and once with normal lighting). After four days the amount of bacteria had increased enormously with many different types of bacteria (rod shaped that looked like elongated colonies of small round bacteria, spiral shaped bacteria, and larger balls that seemed to small to be ciliates. Only very occasionally I saw single celled organisms that were not bacteria (a lone vorticella and something that resembled a very small paramecium). I also left a stick in a jar with a shallow amount of pond water. From that stick I inspected some removed material every day. I found no amoeba as of yet.
Is it possible that the area where you collected your wood stick is particularly abundant with amoeba? Or did you collect your stick from a pond?
Thanks for uploading content so cool
Super cool!
fantastic!!!! is it a simplified version of the agar culture of amoeba ?
I have three questions, Oliver. 1. Would old newspaper instead of tissue do if cut to fit the petri dish and wetted? 2. Should the petri dish be placed by a window for light or in a dark place, or does it not matter? 3. When will your first newsletter be issued?
1. No. Not newspaper. The ink and solvent in the newspaper might do harm. Or allow the newspaper to air out a bit. It the newspaper s smelly (petrol?) then it's not good. As a matter of fact I remember reading somewhere that new newspaper should be used to roll in carpets for storage, as it keeps insects etc away. 2. Light irrelevant, becasue no algae that grow (which would need light). These are heterotrophs, decomposers that feed on organic material and this is their food source. 3. I am almost finished. Maybe in the next few days.
That's really interesting about the newspaper and carpets to keep insects away. I wondered about newspaper because I used to keep a wormery and the earth worms thrived on torn up dampened newspaper.
Thanks, Oliver.
@@Microbehunter: I did the experiment exactly as you said except I did use newspaper. After three days in the petri dish, I made a slide up just as you did with a drop of water and a cover glass on top. I found lots of microbes, but, unfortunately, none large enough at 400x to see any great detail. There was one microbe that appeared in and out of the image at great speed and was too fast to focus on and capture with the camera. I didn't get anything of the quality of image that you got, though, but interesting nonetheless.
I'm trying again, but this time I am using a piece of wetted cardboard cut out of a cardboard egg carton (I am a stickler for recycling stuff), some rotten wood bark, and a single oat for food.
I'm intrigued by the organism that zapped down the right-hand side of the display at 3:19. Were you able to determine what it was please?
Hi am can you please respond to my question, is it safe to grow bacteria or fungus at home and how long can you allow them to grow after they need to be disposed please ?
It all depends on the type. If you make your own yogurt, then you are growing your own bacteria, and this is generally safe. With fungi, it is not healthy to inhale the spores, but also here the type of fungus is relevant. For this reason, one should not grow unknown micororganisms. And even in this video here I have grown unknown microbes and one should be careful. But every time when food spoils, you are growing unknown bacteria and fungi. So I would say, do not grow microorgnisms that you isolated from your own body (eg.skin).
@@Microbehunter okay thanks I also love your videos I am a biology a d chemistry student and I'm studying to later on becoming a surgeon and I get inspired by your videos I recently bought a compound microscope from your advice
How many days before the amoeba are fully cultivated for this method?
Fantastic
Cool vid thank's👍
I am not planning to grow amoeba yet even if my water is 1 year old. I am not sure if amoeba is already here.
Say you had purified water could you theoretically grow these from materials needed for a cell without even using a starter cell
Good evening! I have an idea, you make a ant shot acid and look at it at the microscope? Please try!
Absolut super Oliver
can it work with cultured ones
Could someone explain me in spanish what to do to grow the ameba, please? This is the best video that I'd seen about it, but I don't understsnd what is he saying because he's speaking very fast and my English isn't very good.
Thanks!!
You can write in english too and I'll translate.
it is much better to isolate a amoeba and grow them on agar-plates
What species of amoeba is this ?
I have a question. how can I stop a slide from drying out? like lets say I want to do a microscope timelapse of something growing? is there a trick?
I realize this is a 3 year old post I’m responding to, but I’ll add a comment anyway. I’ve experimented with taking a piece of thread, coating it with a small amount of Vaseline, laying the thread in a circle on the slide, putting the drop of water with your microbes in the center of the circle, and putting the cover slip on top of that. This will allow you to keep the sample longer. The trade-off is that the deeper sample means that critters can swim in and out of focus more easily.
Hi! Can i see cells with eyeobjective- 10× and objective- 100× ?
You can see cells also with 10x and 40x objective. 100x is already very much.
What filters did you use for your microscope to create that kind of colours?
It is called DIC microscopy (differential interference contrast). You can get a similar effect when using oblique illumination.
Did you buy a new microscope then?
Can anyone tell me that how i can watch cilliates at higher magnification , without using cover slip
not recommended. you risk that the objective touches the water. Or you allow water to evaporate, ciliate then dies and does not move. You can then observe at high magnification.
@@Microbehunter thank you for replying 😊
Do you also do like this to observe cilliates??
are some amoeba parasites？
it did not work
It looks like a chromosome 🤔