Thank you for this helpful video. I have one question, to make Sodium polytungstate solution of 1.85 density for 1000 ml water how many g of Sodium Polytungstate do you use?
Thank you for the video tutorial. I have some questions. 1) How long(approximately) will it take to analyze 66 soil samples by your methodology? 2) Are there any possibilities to decrease used water amount in fractionation?
Hello, it has been some time since I have done the procedure, but if I recall correctly, the maximum number of samples we would process in one "batch" was about 16. Any more than that, and it is difficult to finish each step in one day. We generally took three days for the whole procedure, so it would take about 9 days in total for you. With practice, you might be able to decrease water consumption during the rinsing of the LF and the sieving, but you need to make sure you are being thorough. Also, if you do change the amount of water that ends up going to the centrifuge (or use different sized bottles), you will need to calculate the speed and time of centrifugation to separate the correct size particles using Stokes' Law (en.wikipedia.org/wiki/Stokes%27s_law). Good luck!
Congrats fo the video and thank you so much! A couple of questions arise for me: how is the amount of C analyzed in each fraction? Can be used an elemental analyser for this purpose? If so, how to separate organic carbon from mineral carbon? Thanks a lot
You can separate mineral and organic carbon with the use of 2 analyses, separately. This this may lead to the need for a larger sample mass (at least 2 grams, better 5 g). So, you measure carbonates with calcimeter and determine mineral carbon (Cmin). You subtract Cmin from Ctotal, which you measure on CN analyzator by combustion at 900 °C. This is one way to get Corg.
Thanks for the video. Amazing one!
This video was great.
Kindly upload a video on Extraction, Fractionation and Characterization of Soil Organic Matter.
Thank you for the video!
I have two questions:
1) How could we recycle the SPT?
2) How could we cite the procedure that you are describing here?
Thank you!!!
It was extremely helpful .... Appreciate it!!!
Wonderful, thank you!
Please keep up the good work.
I realize it is kind of randomly asking but does anyone know of a good website to stream newly released tv shows online?
@Nico Draven I watch on flixzone. Just google for it =)
@Derek Titus definitely, been watching on Flixzone for since march myself :)
@Derek Titus thanks, signed up and it seems like a nice service :) I appreciate it !!
@Nico Draven no problem :D
Thank you for this helpful video. I have one question, to make Sodium polytungstate solution of 1.85 density for 1000 ml water how many g of Sodium Polytungstate do you use?
Thank you for the video tutorial. I have some questions.
1) How long(approximately) will it take to analyze 66 soil samples by your methodology?
2) Are there any possibilities to decrease used water amount in fractionation?
Hello, it has been some time since I have done the procedure, but if I recall correctly, the maximum number of samples we would process in one "batch" was about 16. Any more than that, and it is difficult to finish each step in one day. We generally took three days for the whole procedure, so it would take about 9 days in total for you. With practice, you might be able to decrease water consumption during the rinsing of the LF and the sieving, but you need to make sure you are being thorough. Also, if you do change the amount of water that ends up going to the centrifuge (or use different sized bottles), you will need to calculate the speed and time of centrifugation to separate the correct size particles using Stokes' Law (en.wikipedia.org/wiki/Stokes%27s_law). Good luck!
Congrats fo the video and thank you so much!
A couple of questions arise for me: how is the amount of C analyzed in each fraction? Can be used an elemental analyser for this purpose? If so, how to separate organic carbon from mineral carbon?
Thanks a lot
You can separate mineral and organic carbon with the use of 2 analyses, separately. This this may lead to the need for a larger sample mass (at least 2 grams, better 5 g). So, you measure carbonates with calcimeter and determine mineral carbon (Cmin). You subtract Cmin from Ctotal, which you measure on CN analyzator by combustion at 900 °C. This is one way to get Corg.
Thank you for the video! I have one question. Did you base your work off literature? If so, can you provide that? Thanks!
Thank you, Is there any chemical which can be used as a substitute for SPT?
You can use NaI (Sodium iodide)
@@TheGirlGreenGrass is it possible to use sodium hexametaphospat to replace the two chemicals?
No one can make you feel better