Excellent video (as always). Please note, the blue/white screening only works if your ligation disrupts the LacZ gene in your plasmid. Not all plasmids contain this gene.
+JM Lee Hi JM. You can measure 20mg of X-Gal based on the concentration of the solution. So if your stock solution is 20mg/mL, then you would need 1mL of stock solution to get 20mg of X-Gal. If you needed 40mg of X-gal, you would use 2mL, and so on.
Excellent video (as always). Please note, the blue/white screening only works if your ligation disrupts the LacZ gene in your plasmid. Not all plasmids contain this gene.
¡GRACIAS!
Thank you so much 😄that was really helpful
Here in this video competent cells are added after plates are made...Can we directly put competent cells along with X gal iptg
so good :-)
Hi, how do you measure 20mg of x-gal when it is in a solution form?
+JM Lee Hi JM. You can measure 20mg of X-Gal based on the concentration of the solution. So if your stock solution is 20mg/mL, then you would need 1mL of stock solution to get 20mg of X-Gal. If you needed 40mg of X-gal, you would use 2mL, and so on.
hello ...
i am doing transformation nowadays ... but i am unsuccessful can you please guide me ???
can I use Glu Gal instead of X-gal?
He reading.
Is it really safe to do this at 2:33?
Yes, but i never do it. It's not really necessary.
Play dno