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SciWris Life Sciences
Индия
Добавлен 8 апр 2020
The channel will post educational videos, tutorials, lecture videos on key subject topics for the benefit of UnderGraduatoin and PostGraduation students.
This channel would be purely educational.
This channel would be purely educational.
Biostatistics: Correlation & Regression
What is Correlation & Regression
How to calculate Correlation and Regression
Video Credits
1. Kanupriya
2. Zoha
3. Vandana
4. Shruti
5. Farheen
6. Pujjvusha
7. Kavita
How to calculate Correlation and Regression
Video Credits
1. Kanupriya
2. Zoha
3. Vandana
4. Shruti
5. Farheen
6. Pujjvusha
7. Kavita
Просмотров: 86
Видео
Biostatistics: IF & COUNTIF Command in Excel
Просмотров 672 месяца назад
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Biostatistics: Variance & T-Test
Просмотров 462 месяца назад
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Biostatistics: Graphical Representation of Mean & Standard Deviation
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Просмотров 185Год назад
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Просмотров 1,8 тыс.2 года назад
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Просмотров 51 тыс.3 года назад
Analysing Protein-Ligand Interactions : Tutorial
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Plant Biotechnology : Steps in Gene Cloning
Identifying Binding Site on Protein : Tutorial
Просмотров 43 тыс.4 года назад
Identifying Binding Site on Protein : Tutorial
Very helpful!, good work
Thank you
Sir, is pymol shows the distance of hydrogen bonding in the interaction. Thanks to show the residue
Yes. Pymol can be used to measure the distance of the hydrobond aswell. I can make a video for that soon.
very helpful and well explained .. thank you so much
You are saving so much time with these videos! Thank you
how top get file of the final result and best complex of docking to use for MD simulation
very good
Can we use this image for publication
What are Gasteiger and Kollman charges? Where exactly are these charges being added in the protein? How can I determine the location of these charges in the protein
Can u make a video on glycosylation reaction by enzyme and substrate in presence of sugar
Very good video!
The video was really thought provoking sir. Thank you
Sir please upload one video for docking for knowing the blinding interaction between protein protein (gene-gene) interaction 8n autodock ..is it possible in it?
L
sir i have one doubt. example pdb id; 1QTF structure not already bound with any small molecule, than how to find binding site of the protein?
How to form trimeric form of protein and ligand interaction
When we require to find the binding site residue, do we have to consider only the residues involved in polar interaction or both polar and non-polar? Pls do reply sir.
Is there any way or software to analyze protein-protein docking results. Kindly guide...?
Thanks for the great tutorial. Can you make a video on how to determine the grid box dimensions? That is the xyz coordinates of the grid box!
VERY VERY HELPFUL !!! 1000 THANKS
Hello. Thank you for your efforts. Will you be having any idea about analysis of polar interactions through VMD of simulation trajectories?
This was so smoothly explained! Thanks!
Sir could you please tell how to identify binding site a protein is not already bound with substrate or any small molecule. The crystal structure is also not available for my protein and I am using alpha fold structure. I want to perform protein-protein dockig and identify interacting amino acids among these proteins. Could you please help?
Registration link: docs.google.com/forms/d/e/1FAIpQLScwZFVqxqbnfYOHKFvjgjbJ0yCPDOBmBq5sK_cRni_-yJV4CQ/viewform
So how many binding sites are there? How to get a definite number of binding site would have been helpful
Hello sir I would really really appreciate if you can do a video on changing amino acids from l to d amino acids in pymol
Thank you sir,
Very very nice video
Please make videos on MD simulations for Windows OS
really great it was very helpful !!!
Sooo helpful! Thank you very much for uploading !
Thank you for your Feed Back :)
Remarkable informative Video sir ...thank you so much.
Thank you for your Feed Back. .
I like the last part
I appreciate your informative and simple guide. I request your instructions via a tutorial video on how to dock heavy metal ligands (like Palladium oxide or Silver) to a protein using Autodock 4.2.
Sir please tell me how to download ligand from pubchem and convert SDF to pdb. .
Hello Sir, How could the ligand move without the protein moving? Thanks
Plz sir call me for next session I have missed this...anyway thankyou.
how does interaction of paracetamol and any protein can be done sir....
You need to perform Protein-Ligand Docking to analyze such interactions.
please sir explain how can I set the grid box dimensions if the ligand I am trying to test (not the original ligand crystalized with the protein) lays far away from the protein (when I input the ligand to AutoDock 4)?? When I tried to cover both ligand and protein inside the grid, the grid box was very big which causes an error message to pop up may times.
Really helpful. Thanks alot.
hello, if i have to compare the functiom of inhibition of 2target cell from inhibitor, what i have to do?? i already get each protein~inhibitor complex, how can i compare these 2 complex??
Thank you sir. Very good explanation
What i do in case of when ligand is not bound with protein ..so how to determine the active site in such case???
You can usually check Uniprot for the active/binding sites. It should give you a residue number which you can highlight in PyMOL.
It really helped a lot!!!! Big thanks..
Thank you sir. it is very much helpful. Sir, if we are taking protein and ligand separately( protein.pdb and output.pdbqt), and trying to find [action-preset-ligands] , i am getting the polar bond for the whole protein, how do i do to get the polar and non-polar residues only nearby the ligand?
Hi Nidhi, You have to use the Original Protein.pdb file that you have given as input. If the protein structure has other heteroatoms, like Water Molecules, and othe co-crystallized molecules, this issue could occur. It can also occur, if you use the protein molecule as protein.pdbqt which is taken from the AutoDock output folder. If this is not the issue, then see if the protein structure is having any issues. Let me know if it resolved.
OMG thank you so much it helped a lot 👍👍
Thank you Leif, Plz continue to support the channel, for the upcoming videos.
i want to visualize a protein protein complex docked pdb file by zooming the specific binding sites for publication.I already did the binding in pymol.But when i try to zoom the binding sites into word the figure is not very clear to see.So kindly help me on this.Thanks in advance
This is really helpful! thanks
Thank you Fauziatul.
plzz upload how to detect active sites of protein in pymol
Hi Ankita, I have already made a video for the same. Plz check the channel for other videos. ruclips.net/video/49q1w0EuuhU/видео.html Identifying Binding Site on Protein : Tutorial Hope it helps.
Sir can you speak on drug designing
Hi Savitha, Yes, I will make a video on this topic soon.