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makes me wonder if 2-ph can be used for killing tumor cells by increasing the pulse frequency to actually heat and denature the cells in the target location

Hello sir, i am using zen blue 3.9, but colocalization option is showing in the left pannel, so can you give me any suggestion, where can i get this option.

Awesome talk!

Oh hi son!🥰

Thank you!

Thank you.

nice pictures, but 0 info on detectors? sad

35:59 made me finally get it

Huge fan of the video! Noticed a small issue on the slide at 10:15 -- it should be λ^-4, not λ^4.

Can you tell me how to do ground truth annotation with black background and a white dot to represent object (mask)

Hi, Thanks for the explanation. what are these different series that open when I am trying to open one image? and how to choose the right series? originally, I was thinking that these series are the images of different samples I have taken on the microscope. if this is the case, then how can I know which image is which? because I have named every image differently for example control, treatment 1, treatment 2. and the series do not mention that.

@Harvard Center for Biological Imaging the macro is giving me an error that line 3 can not start with#@. when I remove this it gives anothe error '.' expected in line 3. i am not programming guy. But what does it mean? I am using imageJ.

Nice and concise presentation. Great job and well done. Very useful. Thanks

super interesting and well explained video, thanks for this!!

nice video ty

Thank you for this nice overview!

i cannot open sample image in FIJI. it shows blank

Do you have any demo videos with actual confocal images... Pls guide

It was an eye-opener for me. Thanks for uploading this educational video.

I enjoyed this and it was helpful learning about the processing pipeline, one thing I didn't see is how to calibrate and I believe this is important and I have not learned how to properly calibrate, do you have any idea how I could learn how to properly calibrate my images?

Great Tutorial! Is there a macro script available? This would be perfect!

Awesome, thanks!

I have an area and want to measure its value. So i put a polygonal figure to the place i want to measure it. I go to analyze than measure. Now instead of a picture, i want to analyze a video with 100 pictures. The place I measure stays the same. How do I do that without pressing measure after every picture?

Can we get spectral line analysis of EDS map data of 5 elements? Is it possible to have y axis in terms of atomic fraction?

17:45

Thank you very much for this training!

Great talk! thanks

Hey, thanks for your video. It was wonderful to learn so much about ImageJ. I just had a query, how to measure the velocity of a rising bubble in any solution using Image J?

Hello I have a query, how to calculate probably paramter for irregularities (PPi)

Thank you very much for the Tutorial! Do you have any advice for automated quantification for IHC-stained muscle fibers? I have to count the positive fibers(not cells) and have been doing it manually. Thank you in advance!

This was a very useful guide! Thank you for uploading it!

What is the software you use to piece things together if you are imaging the brain from both sides (i.e. rotate 180 degrees because of limited working distance)?

Thank you!!

thanks for sharing. It's a pity though video was taken from a far distance and some details are not visible

Super helpful. highly appreciate it.

I took pic with my camera and I have some refexion on one part of the zon I want to mesure. Because of the flash I can not anything. Is it possible to suppress it ?

Apparently blobs.gif is an image of gold particles, not nuclei.

Thanks, that's the first video where the SIM tricks are explained. The audio is horrible, but there is the meat.

Hi! Thank you so much for all the help. I was wondering if the macro at the end is available somewhere? It would help me a lot with my bachelor end project on cell lineage tracing!

Hi. I am wondering if I could use this software to analyze normal pictures? They will come from a normal camera (JPG, Panasonic Lumix DC-FZ80)

legend! thank you

Thanks for that! COuld you please tell me how do I use the erase tool? Right now it is just blackening the area when I am using the erase tool. Not sure why is it so. thanks.

Where can I see the part before this one? Thanks

How do we use it for calculating porosity

Hi. Do you know if there is a way to manually cancel detected 'Blobs' during tracking analysis? or to manually scrub noisy areas so that they are not misidentified during binarization? Thanks

When you say 'memory' at the start you mean RAM (random access memory) right?

To export scatter plot data: Right click on the scatterplot -> Copy or Save data (can be imported directly into Graphing SW)

Thanks a lot for this useful tutorial! In terms of doing batch analysis of multiple images, can the scatter plots be exported? Not just as images, but for import into different software, for example: can scatter plots from different images be combined (for example to show anti-correlation in one set of images and correlation in the second set of images)?

you are amazing, thank you!