- Видео 11
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NMR_DCF
Добавлен 8 сен 2022
Видео
NMRFx (https://nmrfx.org/nmrfx/analyst)
Просмотров 1117 месяцев назад
Advertising this super cool processing/analysis software
STD: Saturation Transfer Difference
Просмотров 1,2 тыс.Год назад
nmrexperimentsdcf.ws.gc.cuny.edu/2023/02/27/saturation-transfer-difference-std/
How to run a TOCSY experiment...
Просмотров 4782 года назад
Differences between TOCSY and COSY; important parameters on a TOCSY experiment.
15N HSQC V2
Просмотров 2882 года назад
How our 15N-HSQC's look like. 2. Decoupled along F1, F2 3. Decoupled along F2 and coupled on F1 (no pi pulse in the middle of the 15N chemical shift evolution) 4. Coupled F1, F2 (no pi pulse in the middle of the 15N chemical shift evolution and no CPD during acquisition) 5. Decoupled along F1 and coupled on F2 (pi pulse in the middle of the 15N chemical shift evolution, but no CPD during acquis...
water suppression (solvent suppression)
Просмотров 1,4 тыс.2 года назад
This is a simple video that shows how to run two different water suppression NMR experiments (zgpr and zgesgp) using TopSpin3.x. Please, leave your comments so I can improve the next ones. Also, let me know if you need something specific.
Creating NMR experiments: Topspin
Просмотров 8922 года назад
This is a simple video that shows how to create simple NMR experiments using TopSpin3.x. Please, leave your comments so I can improve the next ones. Also, let me know if you need something specific.
Thank you for this video and upon request! helpful!
Why change P1 to 7.92?
she calibrated the pulse at 0:39 so she just applied what calibrated value she had onto the parameters on the NOESYpulse program she pulled up. As for why that exact number, not sure. but it makes sense to always calibrate proton 90degree pulse for each sample.
Is a longer d1 good for a lipid and what about the mixing time D8
thank you very much
Helpful! Can you do a video on 90 1H pulse calibration? Everyone may do it different ways but I'd like to get information on what you do that is not 'automated' like pulsecal
Sure: dfavaro@gc.cuny.edu
Dear prof. can I get your e-mail?
Sure, dfavaro@gc.cuny.edu
It's a nice explanation. Thank you! :)
Thank you so much for sharing, Could you please make a video how to take 2D NMR such as COSY, TOCSY and ROESY with water suppression?.
Hi Thong, I'll do it soon!! But it's basically the same thing.. you can check these pulse sequences: roesyesgpph (ROESY), mlevesgpph or dipsi2esgpph (TOCSY), and cosydfesgpph or cosygpprqf (COSY).
Very nice video. If the solution becomes turbid after the ligand interacts with the protein, can I still test STD NMR?
Yes, the only issue is if you have solid particles floating around, once they could give you a bad shim -but you can use a manual centrifuge or wait a bit to remove them from your "reading" area.
@@nmr_dcf6438 Thanks for your tips. Are there any details of the STD NMR manual? For example, Enter commands in order.
Thank you very much. This seems to be very good program to teach the basics to upcoming NMR students
impairutuvely belainne
Very complicated. Please explain simple method or run a water suppression sample so I understand how to suppress a moisture peak in a NMR sample
Well, zgpr is the simplest method for water suppression.
PROJECT pulse sequence: www.nmr.chemistry.manchester.ac.uk/?q=node/479
What are typical frequencys for the on and off resonance experiment?
ON resonance depends on your ligand chemical shift, but usually from -0.2 to 0.8 ppm. OFF resonance --> either -40 or 40 ppm.
Great video! What is the standard NS for the STD experiment on 400 MHz instrument?
Very nice video. I just shared it with one of my students.
Interesting and informative video! Thanks Doc!