Abstract
Enzyme-Linked Immunosorbent Assay (ELISA), a core analytical method in biomedical research, has emerged as an indispensable tool for early disease diagnosis, drug discovery and screening, food safety monitoring, and more. Leveraging its exceptional sensitivity, high specificity, and relatively straightforward operational procedures, ELISA seamlessly integrates the highly specific recognition mechanism of antigen-antibody reactions with the potent catalytic capabilities of enzymes, enabling precise and quantitative detection of biomolecular targets.
Although the fundamental principles of ELISA are clear and operational procedures are trending towards standardization, practical appli...