Видео 21
Просмотров 36 173

What is Biotechnology? What are the types of Biotechnology? || Sheena's Biotech snippets

5:04

What is RNA - seq? || Part 4 || Data Analysis

33:26

What is RNA - Seq? || Part 3 || NGS, Illumina Sequencing by Synthesis SBS, Paired - end Sequencing

39:18

What is RNA - seq? || Part 2 || Y - Adapter Ligation || Bridge PCR / Cluster Amplification

38:20

What is RNA - Seq? || An introduction to RNA Sequencing || Part 1

26:22

What is Fluorescence in situ Hybridization? || FISH || FISH as a physical mapping tool!

20:02

Griffith's Transforming Principle || Transformation || Is DNA the genetic material? Part 1

Hey Everyone, this video describes the experiment conducted by Griffith which led to the discovery of transformation and the transforming principle. This is a back to basics video wherein we will learn about the pivotal experiment that laid the stepping stone to the discovery that DNA is the genetic material.
0:00 Introduction
1:42 What is genetic material?
5:20 Why scientists thought that protein is the genetic material?
6:48 Introduction to Griffith's experiment.
7:57 Different strains of Streptococcus pneumoniae - R strain, S Strain
11:42 Griffith's experiment
12:12 First experimental set- Injecting R Strain
13:18 Second experimental set- Injecting S Strain
14:07 Third experimental set- Injecti...

Видео

What is Biotechnology? What are the types of Biotechnology? || Sheena's Biotech snippets

5:04

What is Biotechnology? What are the types of Biotechnology? || Sheena's Biotech snippets

Просмотров 8713 года назад

What is Biotechnology? What are the types of Biotechnology? Hey everyone! This video gives you a quick insight into the definition of biotechnology and its types based on colours. 0:00 Definition of Biotechnology 0:44 Red biotechnology 1:01 Yellow biotechnology 1:15 Green biotechnology 1:35 Grey biotechnology 1:50 Blue biotechnology 2:05 White biotechnology 2:29 Brown biotechnology 2:57 Gold bi...

What is RNA - seq? || Part 4 || Data Analysis

33:26

What is RNA - seq? || Part 4 || Data Analysis

Просмотров 1,4 тыс.3 года назад

Hey everyone! This is Part 4 of the video on RNA - seq or RNA sequencing. This is an introductory class specifically designed for under-grad students. RNA - seq is a trending technique for transcriptional profiling and differential gene expression studies. RNA - seq is basically carried out in four steps: 1. RNA isolation, RNA species selection and enrichment 2. cDNA library preparation 3. Next...

What is RNA - Seq? || Part 3 || NGS, Illumina Sequencing by Synthesis SBS, Paired - end Sequencing

39:18

What is RNA - Seq? || Part 3 || NGS, Illumina Sequencing by Synthesis SBS, Paired - end Sequencing

Просмотров 1,4 тыс.3 года назад

Hey everyone! This is Part 3 of the video series on RNA - seq or RNA sequencing. This is an introductory class specifically designed for under-grad students. RNA - seq is a trending technique for transcriptional profiling and differential gene expression studies. RNA - seq is basically carried out in four steps: 1. RNA isolation, RNA species selection and enrichment 2. cDNA library preparation ...

What is RNA - seq? || Part 2 || Y - Adapter Ligation || Bridge PCR / Cluster Amplification

38:20

What is RNA - seq? || Part 2 || Y - Adapter Ligation || Bridge PCR / Cluster Amplification

Просмотров 1,5 тыс.3 года назад

Hey everyone! This is Part 2 of the video on RNA - seq or RNA sequencing. This is an introductory class specifically designed for under-grad students. RNA - seq is a trending technique for transcriptional profiling and differential gene expression studies. RNA - seq is basically carried out in four steps: 1. RNA isolation, RNA species selection and enrichment 2. cDNA library preparation 3. Next...

What is RNA - Seq? || An introduction to RNA Sequencing || Part 1

26:22

What is RNA - Seq? || An introduction to RNA Sequencing || Part 1

Просмотров 3,3 тыс.3 года назад

Hey everyone! This is Part 1 of the video on RNA - seq or RNA sequencing. RNA -seq is a trending technique for transcriptional profiling and differential gene expression studies. RNA-seq is basically carried out in four steps: 1. RNA isolation, RNA species selection and enrichment 2. cDNA library preparation 3. Next Generation Sequencing 4. Data Analysis In this Part 1 video, we will be discuss...

What is Fluorescence in situ Hybridization? || FISH || FISH as a physical mapping tool!

20:02

What is Fluorescence in situ Hybridization? || FISH || FISH as a physical mapping tool!

Просмотров 8513 года назад

Hey everyone! This video discusses what Fluorescence in situ hybridization (FISH) is. FISH uses fluorescent probes that hybridize with a target sequence. The position on the chromosome at which hybridization occurs provides information about the map location of the DNA sequence of interest. The positions of various DNA sequences can then be used to map the whole chromosome. Therefore, FISH can ...

What is Restriction Mapping? || Solved GATE Biotechnology Restriction mapping Questions

49:03

What is Restriction Mapping? || Solved GATE Biotechnology Restriction mapping Questions

Просмотров 8733 года назад

Hey everyone! This video discusses how restriction enzymes are used to physically map a genome. Also, I have explained the solutions for all the questions related to restriction enzymes and restriction mapping that appeared in past 10 years of GATE-Biotechnology exam (2011-2019 question papers). 0:00 Introduction 1:02 What is mapping? 1:57 Introduction to restriction mapping 7:28 Calibration cu...

SAGE || Serial Analysis of Gene Expression

35:26

SAGE || Serial Analysis of Gene Expression

Просмотров 10 тыс.3 года назад

Hey everyone! This video discusses the major steps involved in Serial Analysis of Genome Expression (SAGE). Serial analysis of gene expression (SAGE) method is a highly efficient technology that can give a global gene expression profile of a particular type of cell or tissue. 0:00 Introduction 1:18 Concatemer and concatenation 2:10 Biotinylation 4:30 What is SAGE? 9:30 Steps involved in SAGE 10...

cDNA - AFLP || cDNA Amplified Fragment Length Polymorphism

46:33

cDNA - AFLP || cDNA Amplified Fragment Length Polymorphism

Просмотров 1,1 тыс.3 года назад

Hey everyone! This video discusses the major steps involved in the cDNA-AFLP method. Basically a transcript profiling method, cDNA AFLP is Amplified Fragment Length Polymorphism technique performed on cDNA and is used to study differential expression. 0:00 Introduction 1:29 What is AFLP? 2:55 Polymorphisms - types, what gives rise to it? 8:50 What is cDNA - AFLP? 13:43 Step 1- mRNA isolation an...

MALDI - ToF - MS || Matrix Assisted Laser Desorption/Ionization - Time of Flight - Mass Spectrometer

51:43

MALDI - ToF - MS || Matrix Assisted Laser Desorption/Ionization - Time of Flight - Mass Spectrometer

Просмотров 4,5 тыс.3 года назад

Hey everyone! This video discusses the working principle of MALDI-ToF-MS (Matrix Assisted Laser Desorption/Ionization - Time of Flight - Mass Spectrometer) and it's application in the field of Proteomics. The following major topics are discussed: 0:00 Introduction 1:41 Mass/charge ratio 7:02 Mass spectrum 11:17 Components of a conventional MS - General working principle 15:06 Rise of MALDI-ToF-...

Maxam and Gilbert DNA Sequencing Method/ Chemical degradation Method

21:34

Maxam and Gilbert DNA Sequencing Method/ Chemical degradation Method

Просмотров 4054 года назад

Hey Everyone! This video includes: - The chemical degradation method or Maxam and Gilbert method of DNA sequencing. - The explanation on specific chemicals leading to A, A G, C and C T cleavage. - The sequence determination by analyzing gel electrophoresis data. The previous Part 1, 2 and 3 of the DNA sequencing series videos discusses the preparatory steps, principle and procedure of Manual an...

Automated Dye-Terminator Method/ Sanger Sequencing/ Part 3

10:29

Automated Dye-Terminator Method/ Sanger Sequencing/ Part 3

Просмотров 1,1 тыс.4 года назад

Hey Everyone! This video includes: -Automated dye-terminator Sanger's DNA sequencing method. Kindly refer to the previous lessons to understand the principle behind chain termination reaction. This video focuses only on the automation part. -The Part 1& 2 of this series discusses the preparatory steps, principle and procedure of Manual Sanger Sequencing. This channel is being put up to serve as...

DNA Sequencing/ Chain Termination Method by Sanger / Part 2

22:00

DNA Sequencing/ Chain Termination Method by Sanger / Part 2

Просмотров 2794 года назад

Hey Everyone! This video includes: -The 70's method of DNA sequencing- Manual Chain Termination Method by Sanger -This Part 2 video discusses the procedure to conduct Manual Sanger Sequencing. -The introduction and preparatory steps were covered in Part 1. -Automated Sanger Sequencing will be discussed in Part 3 of this series. This channel is being put up to serve as a database for the live le...

DNA Sequencing/ Sanger's Chain Termination method/ Part 1

17:10

DNA Sequencing/ Sanger's Chain Termination method/ Part 1

Просмотров 3394 года назад

Hey Everyone! This video includes: -What is DNA Sequencing -Importance of sequencing -The 70's method of DNA sequencing- Manual Chain Termination Method by Sanger -Discussion on dideoxynucleotides This Part 1 video has the preparatory steps for Manual Sanger Sequencing. The complete procedure is covered in Part 2. This channel is being put up to serve as a database for the live lectures I have ...