- Видео 8
- Просмотров 20 371
seantobiasmay
Добавлен 23 апр 2007
Stuff from the stock centre
Changing an epee tip for beginners
A simple how-to for the club on changing an epee tip and suggestions for making it easier.
Просмотров: 3 452
Видео
Adult drosophila whole brain / CNS dissection animation
Просмотров 8697 лет назад
Made in 1994, this is a step by step animated guide to dissecting an entire adult drosophila central nervous system with specific tips & guidance for reducing strain / distortion. A companion live dissection video has been continuously online since before the modern internet was born. Although the animation tech (and the director/'actor') are now rather old, the actual technique may still be he...
Basic Array concept
Просмотров 207 лет назад
A simple animation used by NASC to demonstrate the principles of array spotting (circa 2001)
Basic array printing concept
Просмотров 97 лет назад
Our simple demonstration animation for the basics of array printing - made for outreach workshops (near the turn of the century).
Old TASII spotted arrayer at NASC
Просмотров 197 лет назад
This is the machine that we used to make our Arabidopsis microarrays back at the turn of the century. We made this training video for use in outreach workshops and teaching.
Adult Drosophila whole brain / CNS dissection
Просмотров 9 тыс.15 лет назад
Dissection of the Adult Drosophila CNS. This is a start-to-finish video showing the dissection of a fruit fly brain. The video begins by introducing the tools and materials and then goes through the process step by step. The method is suitable for downstream staining (e.g. immunohistochemistry or enzymatic) and is intended to be quick. Because the dissection is so quick, it can be (and is) perf...
RNA science
Просмотров 6 тыс.17 лет назад
Running an Agilent RNA gel for Affymetrix work with a soundtrack by System of a Down (Science). The nanochip separates RNA by size. HOW: It is a bit like dropping a piece of flat paper and a piece of screwed up paper - air resistance will make the bigger thing fall more slowly. In this case there is an electrical pull instead of gravity, and there is a semi-solid gel instead of air for resistan...
The NEPS screws are NOT out of LP....they come from a maker in Madrid. LP merely sells them, like many other vendors. One thing I HIGHLY recommend is putting in a flat headed punch -- a 3/32nds for a German wire or the foil end of a reamer for the French style (which includes LP) and GENTLY tapping it a fedw times to make sure the contacts are level, which helps with reliably firing the light. It's been part of my epee wiring for years.
Thanks for the video. Is this a French tip or German tip?
I understand that the process is essentially the same and parts (especially the NEPS screws) are largely interchangeable (unlike foil). These in the video are Leon Paul (British) which their page suggests is a German design.
@@seantobiasmay Only the wire is truly a French pattern...tips from the major makers can be swapped in epee -- a Sport 7 French tip will work in an FWF German barrel just fine.
Is it possible to preserve fly brains in -80 fridge after dissection or does one have to go straight to fixation or live imaging immediately afterwards?
+Samuel Tindell This was made 22 years ago and I moved up the evolutionary scale to autotrophs last century :) - so I would recommend following a more recent protocol for post dissection handling.  However, that said - this video shows a rapid dissection DURING fixation aimed at immunohistochemistry. Fixation helps with structural integrity and opacity of the structure but you need to be quick in order not to overfix. Obviously after fixation (and rinsing) the CNS will be stable in buffer and can be kept. I would not recommend freezing since ice crystals and the thawing process WILL damage the tissue. The same process can be used for living brain but the tissue will be more delicate and the dissection is more difficult. For subsequent electrophysiology you may wish to consider performing the minimum dissection possible - just enough for access/visualisation. I hope this helps.
setting up a gel in 3-5 minutes, not bad! We love our bioanalyzer! For those in the dark the bioanalyzer replaces slab gels for the analysis of RNA, DNA, proteins and even can do cytometry