The Ahmed Lab: Northwestern Neurosurgery
The Ahmed Lab: Northwestern Neurosurgery
  • Видео 19
  • Просмотров 358 750
Ahmed Lab 2021
The mission statement for the Ahmed Lab @ Northwestern Feinberg School of Medicine
Просмотров: 1 292

Видео

GBM Research: An Educational Series
Просмотров 2,3 тыс.4 года назад
Our channel focuses on providing high quality content about GBM research and broader molecular biology research topics. Subscribe for useful educational content!
Gene Modulation: Creating Knockdowns, Overexpressions, and Reporters
Просмотров 1,5 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a detailed overview of how to use plasmids to modulate gene expression in the lab. This technique is used for many research applications as it can help us directly link a gene expression change to a specific cell phenotype and can allow us to test novel gene therapies in the lab. We discuss here starting from a glycerol...
MTT Assay for Cell Viability
Просмотров 104 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a detailed overview of how to perform an MTT cell viability assays. We discuss the theory behind the assay, the potential pitfalls, the step-by-step procedure, and the analysis and interpretation of these assays. All research shown here is from the Ahmed Lab, supported by NIH/NINDS funding. Further protocols can be foun...
Flow Cytometry
Просмотров 2,6 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a basic introduction to understanding how flow cytometry works, how it can be interpreted, and some key considerations in how to perform flow experiments. We discuss the fluorophores available and the different graphs and gatings that must be performed to assess the data. All research shown here is from the Ahmed Lab, s...
Quantitative Polymerase Chain Reaction (qPCR)
Просмотров 28 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 Full protocols for all assays available at ahmed-lab.org This video provides an overview of the theory and practice of quantitative PCR, a widely used technique in molecular biology laboratories in order to measure the mRNA expression of genes or to measure activation of gene transcription for specific genes. Here, we go through both the t...
Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunocytochemistry (ICC)
Просмотров 56 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS Full protocols available on our website www.ahmed-lab.org This video covers the common technique of IF, which is also known as IHC or ICC depending on the type of sample being studied. This is a common and powerful technique used to study protein localization and expression in cells and tissues. All materials in this video are supported by the Ahmed Lab at N...
Western Blot Protocol Overview
Просмотров 15 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD See protocols on www.ahmed-lab.org This video provides a step-by-step overview of the theory and practice of western blotting, which is a technique used in molecular biology to identify the levels of specific proteins of interest across multiple conditions. Here, we go through practical aspects of performing a blot but also discuss the reasoning behind e...
Introduction to Cell Culture
Просмотров 12 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 See protocols on www.ahmed-lab.org This video provides an overview of the steps involved in passaging cells in order to maintain, count, harvest or seed them for future experiments. This is useful for many molecular biology assays as cells form the basis and must be consistently maintained in order to do assays.
Welcome to Wet Lab: Pipetting & Other Basics
Просмотров 3,5 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides an introduction to some basic wet lab concepts for any molecular biology laboratory. It includes a discussion of some general rules of thumb in the lab, understanding the different types of trash, different tubes and tools available, and finally goes through how to set and use various types of pipettes in the lab.
Writing a Scientific Project Proposal
Просмотров 2 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This is a basic overview of how to write a project proposal. It is directed at a simple undergrad or early graduate proposal and gives an overview of key concepts to include. All work discussed or referenced in these videos is supported by the Ahmed Lab at Northwestern's Department of Neurosurgery and funded by NIH/NINDS grants.
RNASeq Overview
Просмотров 10 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video discusses a basic overview of RNA-Seq, explaining how it works and how it is typically analyzed and visualized. Any ongoing work presented here is performed by the Ahmed Lab at Northwestern University's Department of Neurosurgery, supported by NIH/NINDS.
Introduction to R
Просмотров 1,5 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video is intended as a basic introduction to performing analyses in R, directed at new users. All work shown here is performed at the Ahmed Lab at Northwestern Neurosurgery, funded by the NIH and NINDS.
Intro to Proteomics / Mass Spectrometry (MS)
Просмотров 76 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video covers the basics of how to setup and interpret a proteomics screen. All research in this video is performed at the Ahmed Lab at Northwestern's Department of Neurosurgery, and is supported by the NIH and NINDS.
Developing a Scientific Hypothesis
Просмотров 2,9 тыс.4 года назад
Created by Shivani Baisiwala, BS, MS, MD candidate 2021 This video is meant as a basic introduction to how to think about developing a scientific question, creating a hypothesis, and validating that hypothesis. It also briefly goes into how to build up a project from a hypothesis and how to think about validating specific mechanisms. All work shown here is supported by the Ahmed Lab at Northwes...
Introduction to ChIP-Seq
Просмотров 13 тыс.4 года назад
Introduction to ChIP-Seq
Intro to Single Cell Drop-Seq
Просмотров 5 тыс.4 года назад
Intro to Single Cell Drop-Seq
Introduction to GBM Research
Просмотров 6 тыс.4 года назад
Introduction to GBM Research
CRISPR Cas9 Screening
Просмотров 17 тыс.4 года назад
CRISPR Cas9 Screening

Комментарии

  • @xenleopard8631
    @xenleopard8631 13 дней назад

    Great video, really appreciate your efforts

  • @NelsonRudasingwa
    @NelsonRudasingwa 15 дней назад

    Great presentation and sweet Voice 😍

  • @savera3976
    @savera3976 23 дня назад

    I have a question that if i have wild type plant have resistance to some abiotics stress so can be used to overexpress?

    • @ahmed-lab
      @ahmed-lab 21 день назад

      Can you repeat the question clearly? Not sure I understand it.

  • @thehoodedpoet
    @thehoodedpoet 27 дней назад

    Thanks a lot

  • @maielsaid5953
    @maielsaid5953 Месяц назад

    could you please upload the excel template and protocols as I did not find them at the website? and Thank you for your videos please make more<3

  • @vitorquintelas
    @vitorquintelas 2 месяца назад

    Can I use RT q PCR for analysis of the main genes expressed after obtain the protein differentially expressed in MS ? This is a good approach?

  • @VrittaAmroiniWahyudi
    @VrittaAmroiniWahyudi 2 месяца назад

    2024🎉

  • @benedictobalige4858
    @benedictobalige4858 2 месяца назад

    I choose you as my best teacher

  • @ayda.mohammed
    @ayda.mohammed 3 месяца назад

    omg, this is the best video ever about this topic, highly recommended , huge thanks to you lady, I wish you could make more videos to teach us about different molecular techniques , wish you a happy and grateful life ma'am.

  • @KontRiario
    @KontRiario 3 месяца назад

    Wow. It is extremely well explained! Thank you so much! Everything is crystal clear!

  • @sebastianmihardja2113
    @sebastianmihardja2113 3 месяца назад

    Please i need explaination about my mtt assay. My result shown proliferation more than 100 persen.. it is mean good for healing cell or, might become some cancer ? Thanks before

  • @syarifuddinhusain-x3m
    @syarifuddinhusain-x3m 3 месяца назад

    Thank you so much 😭

  • @nancymalhotra6734
    @nancymalhotra6734 4 месяца назад

    Thanks ma'am. This is exactly what I was looking for. You have so well structured everything in single video. God bless you❤

  • @SadiaKhan-l4w
    @SadiaKhan-l4w 4 месяца назад

    so positive control= cell+ mtt agent+ dmso and negative control = mtt+ media+ dmso only?

  • @TinaDelaramParvin
    @TinaDelaramParvin 5 месяцев назад

    This was really helpful, Thank you so much. this video answered a lot of my questions clearly, wish to see more content in this channel!

  • @Islam-nt1mh
    @Islam-nt1mh 5 месяцев назад

    I feel proud of the lab being named Ahmed and I'm really proud of the efforts and great explaination indicating your esteem team would be honored to be part of you one day!

  • @johnlender435
    @johnlender435 6 месяцев назад

    thank you!

  • @APARNATAMANGGURUNG
    @APARNATAMANGGURUNG 7 месяцев назад

    Thank you so much ❤❤

  • @zhansayabauyrzhanova2492
    @zhansayabauyrzhanova2492 7 месяцев назад

    Appreciate!

  • @emreturkmen2662
    @emreturkmen2662 8 месяцев назад

    ı just really hate your voice the tone of your voice the way of your talking it just makes me sick

  • @VV-fw1tw
    @VV-fw1tw 8 месяцев назад

    Thank you so much for your clear explanation 🙏. I have learnt quite a lot from you !

  • @BenjaminBaahKonadu
    @BenjaminBaahKonadu 8 месяцев назад

    Thank you very much!!!!

  • @josephpareti9156
    @josephpareti9156 8 месяцев назад

    'ChatGPT for CRISPR’ creates new gene-editing tools - is this the way to go ?

  • @benjaminsulemankurya6664
    @benjaminsulemankurya6664 9 месяцев назад

    wow. This is clear to follow and understand RNAseq for beginers. I save the video

  • @柯坦-s5d
    @柯坦-s5d 9 месяцев назад

    Thank you for making this helpful video : )

  • @YuetongUBC
    @YuetongUBC 9 месяцев назад

    Thank you very much for your video! a small question: Is it possible to do the mRNA with a small sample size (e.g. 100,000 cells) for non-housekeeping genes?

  • @johnbewty
    @johnbewty 9 месяцев назад

    This was wonderful. Very appreciated.

  • @naimehrafatian3946
    @naimehrafatian3946 11 месяцев назад

    it is the best video of the technology I saw so far

  • @adedoyinadeyemi-zr6my
    @adedoyinadeyemi-zr6my 11 месяцев назад

    you really really demystified RNA seq....super easy to understand....Thank you☺

  • @aysekaymaz6046
    @aysekaymaz6046 11 месяцев назад

    Great explanation! Very helpful

  • @bilalraheemabdulraheem8445
    @bilalraheemabdulraheem8445 11 месяцев назад

    Thanks alot

  • @ctrlaltdelicious6072
    @ctrlaltdelicious6072 Год назад

    Wow this was the most useful video on transfection/transduction/CRISPR I have ever seen and I am so thankful for content like this. I am preparing for a job interview :)

  • @seunmustapha6102
    @seunmustapha6102 Год назад

    I watched several videos on IHC, IFC, ICC. The is the best i have seen. This is succinct, practical, and coherent. This is excellent. I was a 60mins that felt like 5 mins. Thanks a lot for this masterpiece

  • @sarahhepler8654
    @sarahhepler8654 Год назад

    Can MTT be used for virus quantification?

    • @ahmed-lab
      @ahmed-lab Год назад

      No. However, it can indirectly measure toxicity induced by virus infection.

  • @JulieKatherineCho
    @JulieKatherineCho Год назад

    Love how thorough this video is! Would you by chance consider another follow up video with immunohistochemistry dilution calculations? I'm in undergrad, and would love to know this before volunteering at a lab!

  • @usava_group
    @usava_group Год назад

    Thank you so much for such a great tutorial. Super helpful and easy to understand.

  • @Viktoria-cx5xw
    @Viktoria-cx5xw Год назад

    Thank you so much, good explaining ❤❤

  • @hystrionic
    @hystrionic Год назад

    i'm interested in the info but the vocal fry is making it hard to listen.

  • @bahar_e991
    @bahar_e991 Год назад

    Thank you

  • @shawsheryl5092
    @shawsheryl5092 Год назад

    So clear! Thanks!

  • @biggjones001
    @biggjones001 Год назад

    I loved this totally! And a great voice to go with the explanation. Thanks

  • @trabdbioquimica
    @trabdbioquimica Год назад

    Such an amazing video!

  • @fgfanta
    @fgfanta Год назад

    Clearest explanation of ChIP-seq I have come across so far.

  • @usava_group
    @usava_group Год назад

    Thank you so much for such informative and easy-to-understand videos. Appreciate it a lot.

  • @saymaafrin5927
    @saymaafrin5927 Год назад

    Very helpful. Thank you!

  • @maki-chanyagami6842
    @maki-chanyagami6842 Год назад

    big help, thank youu!!

  • @Adi64717
    @Adi64717 Год назад

    Best explanation! Thank you!

  • @Adi64717
    @Adi64717 Год назад

    This is one of the best biochemical videos I have seen. Thank you so so so much.

  • @preciouschukwuwubem9740
    @preciouschukwuwubem9740 Год назад

    Great content.

  • @basyalpalpa5680
    @basyalpalpa5680 Год назад

    ❤❤❤ exceptional