- Видео 15
- Просмотров 79 888
Crash Biology
Добавлен 21 май 2017
My name's Sophie Tsai and I had originally created this channel back in 2017 when I was in graduate school at Simon Fraser University in Burnaby, BC. I've always loved teaching, and while most other graduate students thought having to TA was a waste of time, I really enjoyed connecting with and educating my students. This channel was a nice break and reprieve from the daily stress and woes of grad school, lab work and the all too many failed experiments and troubleshooting one goes through in research!
I've since graduated in 2019 with my MSc Molecular Biology & Biochemistry and work full time at a Canadian biotech. I've taken a long break from RUclips, but hope to be back now as I really enjoy making these videos. Drop me a comment! I love seeing how far my videos reach. :) - Sophie
P.S. The channel used to be called BehindTheBench, but as of November 2021, I've rebranded it to Crash Biology as I think it fits the style of the channel better.
I've since graduated in 2019 with my MSc Molecular Biology & Biochemistry and work full time at a Canadian biotech. I've taken a long break from RUclips, but hope to be back now as I really enjoy making these videos. Drop me a comment! I love seeing how far my videos reach. :) - Sophie
P.S. The channel used to be called BehindTheBench, but as of November 2021, I've rebranded it to Crash Biology as I think it fits the style of the channel better.
Interphase / Cell Cycle Checkpoints / Cell Biology
The cell spends the majority of its time in interphase. Here, I talk about G1, S, G2, and G0 phases and go through the purposes of the three checkpoints.
0:00 Intro. Interphase overview
0:38 G1 phase
1:42 G1 checkpoint
3:35 G0 phase
4:00 S phase
6:02 G2 phase
7:12 G2 checkpoint
8:15 M checkpoint
10:19 End
0:00 Intro. Interphase overview
0:38 G1 phase
1:42 G1 checkpoint
3:35 G0 phase
4:00 S phase
6:02 G2 phase
7:12 G2 checkpoint
8:15 M checkpoint
10:19 End
Просмотров: 179
Видео
Fetal Hemoglobin vs Adult Hemoglobin & 2,3-BPG. How a fetus gets its oxygen from mom.
Просмотров 2,2 тыс.2 года назад
Explanation of the structural difference in fetal hemoglobin vs adult hemoglobin and how 2,3-BPG/2,3-DPG effects hemoglobin's affinity for oxygen. 0:00 2,3-BPG/2,3-DPG structure 2:38 Function of 2,3-BPG 4:10 HbA vs HbF and effect of 2,3-BPG 6:05 Hemoglobin binding curve 8:36 Summary of HbA vs HbF difference
Stages of Meiosis Explained
Просмотров 2,3 тыс.2 года назад
Meiosis: Reduction of ploidy by half, introduction of genetic variation, pairing of homologs, crossing over - all to produce 4 unique haploid cells. 0:00 Introduction / Interphase 1:31 Prophase I / Homolog Pairing / Crossing over 3:19 Metaphase I / Law of Independent Assortment 5:11 Anaphase I 6:03 Telophase & Cytokinesis I 6:50 Meiosis II / Prophase II 7:23 Metaphase II 7:53 Anaphase II 8:19 T...
How two mutant parents produce normal offspring - Complementation Testing / Genetic Complementation
Просмотров 8162 года назад
How genetic complementation works. How is it possible that two mutant parents can produce a wildtype phenotype? Let's dig into the genetics and pathway of it all! 0:00 Channel Rebrand 0:40 Definition of complementation 2:40 Fly eye colour pathway 3:39 Example 1 5:36 Example 2 (Complementation!) 7:04 Example 3 8:39 Quiz Questions 9:25 References
Rapidly making billions+ copies of your target DNA sample - PCR explained
Просмотров 2142 года назад
Hi! It's been a while since I've made a video! Missed making these for you all. Just got access back to my editing software. By the way, I rebranded my channel from BehindTheBench to Crash Biology to reflect the content of the channel better - new name and logo, same ol' me :). PCR was a HUGE backbone of my Master's thesis when I was doing a ton of cloning, so believe me when I say I've run pro...
Combinatorial Control
Просмотров 7 тыс.5 лет назад
In this video we discuss how both phenotypes and gene expression are controlled by combinations of elements. An example we use is the ABC flower model, below I'll link a more in depth ABC model video we made. ruclips.net/video/yHYVRJ_ORUU/видео.html
Morphogens Vs Signals
Просмотров 10 тыс.5 лет назад
Here we discuss what a morphogen is, and what distinguishes it from other signals. See the link below for a more detailed explanation of morphogen involvement in Drosophila development. ruclips.net/video/ZOzKXrOGtgw/видео.html
Somite Formation & 90min clock
Просмотров 20 тыс.5 лет назад
Discussion of how somites form in chick embryos and how the 90min clock works in relation to negative feedback loops. Typo in video I've reversed A B accidentally on the ABC flower model diagram. B should be on top and A on the left. However the principle of combinatorial gene expression still applies! Check out out ABC flower model below for a more in depth explanation. ABC Flower Model: rucli...
![Drosophila Embryogenesis - Anterior/Posterior Patterning [English Captions]](/img/1.gif)
Drosophila Embryogenesis - Anterior/Posterior Patterning [English Captions]
Просмотров 23 тыс.5 лет назад
Developmental Biology: Anterior/Posterior patterning of drosophila embryo and discussion of 4 maternal effect genes: bicoid, nanos, hunchback, caudal and their concentration gradients. English captions are available if you'd like to follow along as I speak for better clarity etc.
Enzyme Kinetics Review
Просмотров 2476 лет назад
A review on enzyme kinetics originally prepared for my Molecular Biology & Biochemistry undergrads. I made a small error in the video while I was doing the voiceover! At 8:14, I mean to say rate 1 rate -2 rather than rate 1 rate 2. :)
Protein Chromatography: Ion Exchange, Gel/Size Exclusion, Affinity
Просмотров 3,1 тыс.6 лет назад
We go over the three main types of chromatography to separate your protein of interest. We got a fancy new drawing tablet so all our drawings will be digital now instead of on a whiteboard :). Artist: Kyle Simpson Narrator: Sophie Tsai
Pipette Techniques
Просмотров 1,2 тыс.7 лет назад
Here we show you the basics of how to read and use micropipettes! This is an incredibly important tool for all sorts of molecular biology, and even in early undergrad you'll find this useful.
Nucleic Acids: DNA vs RNA. Differences discussed.
Просмотров 6077 лет назад
Here we explain the differences between DNA and RNA!
ABC Model of Flower Development
Просмотров 2,7 тыс.7 лет назад
In this video we will discuss the process by which floral structures develop, the ABC model!
Drosophila Embryogenesis - Morphogen mRNA and protein gradients explained
Просмотров 6 тыс.7 лет назад
This one's for you developmental biology students! We explain mRNA and protein levels of morphogens in Drosophila: bicoid, nanos, caudal and hunchback. Studio lighting was graciously provided by Steven Barrett :).
Thank you for this great video. Your explanation was very clear
Thank you. This was amazing .
So glad it helped! That's what I love to hear. - Sophie
Pls can I explain how the parasegments along the anterior posterior axis are offset so that int he adult fly they’re in opposite sections
Thank you Sophie, your explanation was super helpful, easy to understand and makes it easier to remember! The graphs were very helpful too and thank you for repeating the important aspects.
Patti - you are so welcome. So glad to hear you found it helpful! These are the comments I love to see! ❤️ - Sophie
nice idea of morphogens, thanks you very much bro
Thanks for the awesome comment! I'll pass this onto Kyle, who recorded this video back in our grad school days! - Sophie
perfect!
Thanks so much! :)
Thank you so much for your beautiful work and clear explanation! You are so generous and really great at teaching.
You are so welcome!! Thanks so much for your kind comments :). They make my day! ❤
thanks！！！
You are so welcome!!
só achei esse vídeo sobre o assunto e consegui entender perfeitamente, explicação muito boa!
Muito obrigado Giovanna.
Hi, I think that the LOF of gene C mutant would produce a sterile plant because only sepal and petal genes are present. By the way, I would like to say thank you, your video really helped me a lot.
nice 👍
I love you for this well-scripted video !!!! i understood everything
:) I'm so happy to hear that! Glad it helped. Good luck on your exams and courses!! - Sophie
You are a fantastic teacher! I am a visual learner and struggle in my Developmental course due to the lectures being a bit disorganized. Thank you so much for your taking the time to make this video and good luck on your future endeavors!
Thank you so much! This means a lot :). I always love seeing how much my videos help students.
There are so few videos on this. Thank you for explaining this in very understandable terms for this grad student
I gotchu haha. Developmental bio is hard, so glad I can help :) - Sophie
very well explained
Thank you so much! It's really helpful
OMG, that was super helpful. Those graphs and your lovely explanation made all of it super clear and memorable.
Thank you so much! Comments like these make me sooo happy, and is the reason I started this channel in the first place. ❤️
oh my god thank you,
Omg I’m so happy my videos are still helping people! Have a great weekend! - Sophie
@@CrashBiology thank you, I have exam on Tuesday and this topic is included but I was so confuse and luckily I found this video I understand it so well
@@afnansaleh6145 awesome!! Good luck on your exam. Go crush it! :)
what's the difference between a signaling molecule and an inducer?
간호학과 강의 도움 되어 감사합니다
So happy I could help. That means a lot. ❤️🥰
@@CrashBiology 네~^^ 앞으로도 강의 듣고 열심히 공부 하겠습니다~^^ 수고많으십니다~^^
You made understanding these concepts very much easy. Thankyou. This channel is underrated
This makes me soo happy hearing this. :) thank you so much!
1) headless 2) head in the middle
jazaakallaahal khair , from india
Hi, I'm an MSc. student who is new to microbiology and these techniques. Do you offer support in that regard?
What type of support are you looking for?
@@CrashBiology I would like to know where I can access materials that would better help me to understand the processes involved in the Molecular Identification of organisms like fungi, as I don't really understand what processes like barcoding, genomics and so on are. Also do you offer private classes and how much would it cost?
@@bluejay9220 So genetic barcoding is when you've created a library of DNA sequences that can be used as a reference. Say you now have a new organism and you've isolated DNA from it, but you don't know if it is already an identified species. So you PCR it and sequence it and compare it to your library to identify it. I'm so flattered but I do not offer online tutoring as I'm already super busy with my full time job. I just make these videos on the side as a hobby. :)
@@CrashBiology Thank you for your feedback and time. Your answer is really insightful. I'll keep an eye out for more of your videos.
@@bluejay9220 Thank YOU for watching :). Always makes my day when people enjoy my content.
text
This was so incredibly helpful. Could you do a video on Lineweaver-Burk Equations?
I'll add it to the list of topics. I'll get the channel back up and running soon :). Compiling topics now and getting access again to my video editing software. Should be a few more weeks since I'm quite busy.
Also come back!!!!!! Your work is needed, you make great videos ❤️
This was the nicest comment ever! what videos do you want to see? After I finished my contract for video production i had to give up the licence for my video editing software but I’m sure I can look around for a free one and get back to making videos. - Sophie ❤️
@@CrashBiology Aw your welcome! I am thinking about G-protien, signaling processes (down/up stream), and common signaling complexes in the human body. I know that might be a lot but I am struggling with wrapping my head around these basic processes. I know with some visuals and some facts here and there about how these process naturally affect the body would really help me out with my MS program. :D Also, sorry to hear your having to give up your software rights hope you can get a better program soon! <3
If possible could you give the answer to review question 2? I believe it has to do with pre-determined cells but I am not quit sure how the orientation effects the development ...
The timing of somite formation will always follow what was originally specified! So they will go out of order. - Sophie
@@CrashBiology Ah very fascinating that even orientation can affect the developmental process. Thank you for answering! sorry for the late reply my MS has carried me away! If possible could explain why the orientation changes the direction of the ribs? why don't the cells re-orientate?
Me he enterado mejor con esta señora hablando en ingles, idioma del cual no tengo ni el B1, que de mi profesora. gracias!
Gracias mi amiga. Estoy felíz de ayudar :)
Ok
Thanks a lot! The best video on the topic
omg thank you !
Do you have a reference for the gene regulatory network shown at ~2:11?
I have the same question
Thx❤
the best explanations are on a whiteboard, thank you
really good summarisation and explanation! everything clicked
Thanks
lol my tuition money should go to you instead cause i learned more from this quick 10 min vid than any of lengthy lectures.thanks i rlly appreciate this video!! it was so well communicated and easy to understand - you are a natural educator.
thank u for this clear concise answer!!
wow you did an exceptional job explaining this concept! Thank you very much.
Really educational <3 Thanks
Hi, i know it is late but can I know more about regulation on higher organism? I know that you have given flower as an example. But can you be more specific with that part?
thank you! very clear to understand
Such a good video ~~ thank you!
Awesome to hear that Grace. Sorry for the late reply. Im not on this account much anymore as Ive graduated from my Masters program and now work full time. All the best with your studies though :)
From sudan thank you it is helpful with my dev biology exam
Amazing to hear Abdelrahman. Apologies for the delayed reply. I dont check this account as often due to having graduated from my Masters program. I now work full time! All the best with your future studies!
Thank you! This was so clear!
Very clear, thank you. What are the concentrations of RA and FGF traveling in? Totally extracellular goo? What are the main molecules? Are there experiments showing that anything (like temp) could slow down or speed up the clock? If so is it by affecting the movement of RA and FGF, or the cycle time of the neg feedback loops?
Insanely helpful video! So informative but easy to follow, I've been reading for days only to understand it better in minutes thanks to you!
Hey - thanks so much! It means a lot to hear that my video is helpful! I'm not very active on this account anymore as I've finished my MSc degree and now work full time, so apologies in replying to you so late!
@@CrashBiology No worries! Congrats on finishing your degree! Want to say thanks again, I can now tell you you helped me smash out one of my exams!
bluenosedrabbit that’s amazing!! So happy to hear that! I work in biotech now and we’re actually talking about making some educational videos similar to ones like these! I think Kyle has transitioned to a PhD program! How time flies. Feel free to add me on LinkedIn under Sophie Tsai, MSc if you want to build your network. Best of luck with your studies :). - Sophie
very nice and clear explanations! easy to follow
Thanks for video