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Hello I have a question I want to count hair graft with imageJ ( i have numbers of grafts but when I use this app the results is not correct ) Would you please help me ?

Hi what magnification did you get your image on?

Thanks

I would like to count just pairs of cells (2 cells that are close to each other and no cells which are standing single) Do you have any clue how to do that with ImageJ?

Thanks for the video! It's excellent! I have one question: I have to quantify some PI (propidium iodide) images of hippocampal slices... how do I get the percentage of dead cells for the total tissue area? For that, how do I get a sum of the area of ​​all dead cells and divide by the total area of ​​the slice? Preferably in a quick way without having to mark all dead cells. Thank you!

nice info, but how to cite in journal?

Nice video, I want to count spherical shape cells. But the sizes vary from each other might be small as 10um diameter to too big diameter. Can you help me to count all the cells

Do I buy a glass slide with a well and inject my stained sample into this well prior to taking photo.

Kevin, How does someone acquire the photograph of a sample that is stand. I mean if I want to count cells per ml, how do I know how much sample is in the photo. I know people say use a hemocytometer but is there a glass slide with a groove that takes a desired amount of sample or something other than a hemocytometer. I would have thought a hemocytometers lines would mess up the image taken from a microscope.

How does the "Fill holes" algorithm at 2:07 work?

Nice video.

Thanks alot! Fill holes doesn't work well for my particular images - the middle area of cells disappears completely and the outer cells don't change. Eh. It still is working well - I used the 'record' feature to make a macro to automate it and it works like a charm

Thanks so much. Please I do I calibrate my sample. Please how do I record my my values

Gracias!!

Thank you so much! ❤️ This really helped me a lot ☺️

Hello Kevin Thanks for the video. It is very informative. Do you think the image file must be (tif) in order to use the watershed function and subsequently you can count the objects using the analyze particle command?

Thanks very much for sharing! this is very helpful!!!!

Hi, I have a question and hope to get my answer from you. Actually, I have some pictures (with green florescent dots as cells) randomly taken from my sample. I counted the cells/picture by ImageJ and I was wondering how to extrapolate these certain number of cells counted as it's very hard to take lots of pictures from my samples by a florescent microscope.

And how do you actually count the number of cells??

What is the size of the cells? I mean, the diameter.

What exactly does the image show?What kind of cells?I am writing my thesis and it could be very helpful to have more information about the picture.Thanks a lot in advance.

Thank you for explaining how to process an image through ImageJ and uploading a video about it! and I believe you need to revisit your video whenever there are new comments to answer them...again thanks!

Thank you so much for your woderful tutorial. I have a question. How I can know the exactly threshold?

Outstanding Tutorial! It just solved my problem. Thanks a lot, Kevin. :)

You saved my master degree

if I want to erase something, how do I do that?

Thanks Kevin. It made my job a lot easier.

thx for the tutorial. btw, i have an image that consisting of dirt. if dirt > 0.04 mm need to count, if below 0.04 mm not count. where i can put this parameter? thx

Thank you very much for the great video

thanks...

Many thanks for the video, it helps me a lot. I wonder that if I have cells stained with Hoechst, and I want to count dividing cells with 2 nuclei close to each other (at anaphase or telophase) as 1 cell, what should I do?

Thank you for the informative video. Is there any way to batch this if we have many samples to count?

Hi! Thanks for the video. When I put the watershed part my software is just binding black points... Its not working as the video said.. can you help me?

Hi. Thanks for your sharing. It's so helpful! I have a question about watershed. I can't separate cell one by one. Are there some better method to separate cells?

hi, could you please give me the link of microscopic blood image..

Hey guys! Wish this message finds you well. Is there anyone here have the text tutorial of this software so he could share it with me via my e-mail please? I need it a lot to estimate the severity of bean anthracnose for my thesis. Thank you in advance!

Hi. Thanks for the video. How can I use imagej to count islets of langerhan?

Hi there. I'm looking for a program to count cells, but this program doesn't seem to do the trick. I have images of a micro-array covered with wells and I only want to count cells _inside_ those wells (which are regularly interspaced as if located on the vertices of a grid). In other words, I need the program to only count cells on particular pre-selected locations of the picture. Any advice there?

Thanks ..................

It was really useful. Thanks a lot!

Thank you very much! Very useful

Thanks so much!!

If someone can tell me how to count stomata; that'd make my life waaaayyyy easier! ;)

That's sooooooo useful!! Thanks A LOT!!

This is what I looking for.. Thank you

You are amazing thanks a million!!

Hi Kevin, good job, I was wondering if it is possible to count cells in more than one image at once. for example, I have 200 images and I want to cell counts from each image at once. Thank you in advance!

Nice tutorial video, it's very informative. I have a question if you don't mind. Is there a functionality or option in ImageJ that allows the user to count the number of pixels of a particular color and/or get its percentage from the total number of pixels? For example, I have an image with color A and B, I want to get the total number of pixels of color A, or color B. (For simplicity, we can let color A = black, and color B = white) I would really appreciate if you could respond to my queries, it will be a great help. Thanks in advance.

hi Kevin, may I ask if I can choose the area I am interested in to count the cell number? do I have to count all the cells in the figure?

Thanks very much! It's really helpful.