Spectral Solutions
Spectral Solutions
  • Видео 25
  • Просмотров 182 715
Calculating Ligand Binding Kd Using NMR Data | Step-by-Step Tutorial
“In this video, I provide a detailed guide on calculating the dissociation constant (Kd) for ligand binding using NMR spectroscopy data. This tutorial covers the fundamentals of binding affinity, interpreting NMR data for Kd calculation, and step-by-step calculations to derive accurate results. Reference paper: www.sciencedirect.com/science/article/pii/S0079656523000122
chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/eprints.whiterose.ac.uk/190658/1/s10858-022-00402-3.pdf
Просмотров: 21

Видео

DOSY NMR data processsing by Topspin and Dynamic Center
Просмотров 8 тыс.5 лет назад
DOSY NMR data processsing by Topspin and Dynamic Center
NMR assignment of protein or peptide by Cara software
Просмотров 4,2 тыс.6 лет назад
NMR assignment of protein or peptide by Cara software
2D Hetero nuclera NMR data plotting with projections
Просмотров 3 тыс.6 лет назад
2D Hetero nuclera NMR data plotting with projections
2D Hetero nuclear NMR data processing HSQC and HMBC
Просмотров 10 тыс.6 лет назад
2D Hetero nuclear NMR data processing HSQC and HMBC
Processing multiple 1D NMR spectra's at ONCE
Просмотров 1,9 тыс.6 лет назад
Instead of processing one by one 1D NMR data you can process multiple NMR spectra by using the command "multicmd" at once with processing parameters.
Extracting 1D NMR spectras from 2D/psuedo 2D NMR data
Просмотров 2 тыс.6 лет назад
Extracting 1D NMR spectras from 2D/psuedo 2D NMR data
Opening NMR data in Topspin (Creating Directory and without Directory)
Просмотров 21 тыс.6 лет назад
Opening NMR data in Topspin (Creating Directory and without Directory)
NMR automatic assignment and structure analysis by Bruker CMC Assist
Просмотров 7 тыс.6 лет назад
By using the CMC Assist software you can predict the structure and from structures to assignments
NMR Magnet Quench
Просмотров 3,2 тыс.6 лет назад
See How liquid Helium will come out from the NMR Magnet...
2D NMR: External Projections setting
Просмотров 2,5 тыс.6 лет назад
2D NMR: External Projections setting
2D NMR: Peak Picking
Просмотров 2,1 тыс.6 лет назад
2D NMR: Peak Picking
2D NMR: Editing contour leves
Просмотров 7506 лет назад
2D NMR: Editing contour leves
1D NMR: Peak Picking
Просмотров 5256 лет назад
1D NMR: Peak Picking
1D NMR Spectrum Integration
Просмотров 4686 лет назад
1D NMR Spectrum Integration
1D NMR and 2D NMR data Phase correction
Просмотров 4,1 тыс.6 лет назад
1D NMR and 2D NMR data Phase correction
Removing T1 noise and offset noise from 2D NMR Spectrum
Просмотров 2,6 тыс.6 лет назад
Removing T1 noise and offset noise from 2D NMR Spectrum
NO-D (Deuterium) NMR for Chemical Reactions
Просмотров 8106 лет назад
NO-D (Deuterium) NMR for Chemical Reactions
Protocol for NMR analysis
Просмотров 4726 лет назад
Protocol for NMR analysis
1D NMR data processing (Multidisp, stack plot) by Topspin
Просмотров 25 тыс.6 лет назад
1D NMR data processing (Multidisp, stack plot) by Topspin
Complete 1D NMR Data processing(Integration, peak picking, PDF) by Bruker Topspin Software
Просмотров 40 тыс.6 лет назад
Complete 1D NMR Data processing(Integration, peak picking, PDF) by Bruker Topspin Software
Hydrogen Bonding study by using NMR
Просмотров 5566 лет назад
Hydrogen Bonding study by using NMR
NOESY Vs ROESY
Просмотров 3,8 тыс.6 лет назад
NOESY Vs ROESY
2D NMR data processing (phase, baseline, pdf and image) by Topspin
Просмотров 19 тыс.6 лет назад
2D NMR data processing (phase, baseline, pdf and image) by Topspin
Bruker Topspin Software installation
Просмотров 20 тыс.6 лет назад
Bruker Topspin Software installation

Комментарии

  • @rameesk4824
    @rameesk4824 5 дней назад

    how can I contact you? I have a few doubts regarding the processing

  • @rameesk4824
    @rameesk4824 Месяц назад

    very useful videos. I need to ask you regarding heteronuclear NMR. How can I contact you

  • @JonahRoland-n5s
    @JonahRoland-n5s Месяц назад

    Jamaal Plains

  • @CandelariaReyes-c2c
    @CandelariaReyes-c2c Месяц назад

    Haag Point

  • @thetorque5459
    @thetorque5459 3 месяца назад

    Very very useful... thank you sir

  • @kirthijoshi8548
    @kirthijoshi8548 6 месяцев назад

    Hi sir, could you please make a video on how to plot and export overlayed 2D spectra

  • @ru1h
    @ru1h 6 месяцев назад

    How are you saving it to the portfolio because when I try to plot only one shows up

  • @ayahasan7330
    @ayahasan7330 7 месяцев назад

    Thanks a lot. Can I make the width of the peak less?

  • @aruyukimayu1199
    @aruyukimayu1199 7 месяцев назад

    Thank you. It helped us with a dataset of 1000 data.

  • @srishtijha7357
    @srishtijha7357 8 месяцев назад

    How to calculate peak width

  • @Moalpad
    @Moalpad 10 месяцев назад

    Thank you !!!

  • @NAZIMUDDIN-px4rm
    @NAZIMUDDIN-px4rm Год назад

    good

  • @kakarlapakeeraiah2580
    @kakarlapakeeraiah2580 Год назад

    While entering the structure it's not showing anything

  • @kakarlapakeeraiah2580
    @kakarlapakeeraiah2580 Год назад

    Sir i am using CMC assist how to predict the structure can you please assist me sir

  • @kakarlapakeeraiah2580
    @kakarlapakeeraiah2580 Год назад

    Sir can I get ur contact number

  • @gorbesiah8
    @gorbesiah8 Год назад

    Where did you get `.seq` format file? We have FASTA, but not .seq. EASY program is no longer available.

  • @md.sydulislam4362
    @md.sydulislam4362 Год назад

    Not helpful for beginners!

  • @fnj9567
    @fnj9567 Год назад

    Great tutorial! Thank you so much

  • @agatafullman2529
    @agatafullman2529 Год назад

    This is so helpful, thank you!

  • @brianshiue3009
    @brianshiue3009 Год назад

    thanks bro

  • @stephenoreilly5696
    @stephenoreilly5696 Год назад

    Dude, thank you so much! Writing my thesis and was trying to work this out for ages!

  • @jigneshpatel6044
    @jigneshpatel6044 Год назад

    Can ple sir share noesy exeperiment done in topspin

  • @priyagupta-yt8rg
    @priyagupta-yt8rg 2 года назад

    If u are making a tutorial video then try to go a bit slower...it's not like everyone is already familiar with all the commands

  • @karthik24
    @karthik24 2 года назад

    video and voice is somewhat non sink

  • @syedkabirhussainshah3310
    @syedkabirhussainshah3310 2 года назад

    Processing commands are not working in case of my ROESY spectrum and after doing processing using the BC mode and Q fill I am getting worse spectrum

  • @soumitamukherjee1773
    @soumitamukherjee1773 2 года назад

    Please make a detail video for topspin 4.1.4

  • @olie7218
    @olie7218 2 года назад

    When I opened my NMR file, a windoew showed up and said "This is an incomplete data file path (or the data path contains invalid data)." Do you know how to deal with it? Thank you.

  • @Kapgabo
    @Kapgabo 2 года назад

    I have a Problem, maybe you can help me. If I click on the projections for top and left, it is not adding the spectra. It seems like it is not "linked". What should I do?

    • @Kapgabo
      @Kapgabo 2 года назад

      Hahah should have watched the Video till the End 😅 thanks!

  • @short9892
    @short9892 2 года назад

    following

  • @pinghe9788
    @pinghe9788 2 года назад

    is that possible to show chemical shift using topspin on the spectra after you did the peak picking?

  • @ayodeleoluwatosin7246
    @ayodeleoluwatosin7246 2 года назад

    Kindly guide me on how to calibrate axis for both proton and carbon in HMBC?

  • @ayodeleoluwatosin7246
    @ayodeleoluwatosin7246 2 года назад

    Thanks so much

  • @Veenavikram
    @Veenavikram 2 года назад

    Thank you so much... this video helps me a lot.

  • @danielnilson6115
    @danielnilson6115 2 года назад

    I appreciate this so much

  • @sashanealand8315
    @sashanealand8315 2 года назад

    you cannot automatically generate structure in the academic version it is blocked

  • @fefestalex
    @fefestalex 2 года назад

    THANKSSSSS

  • @cyntiaalves9255
    @cyntiaalves9255 2 года назад

    I'd like to change the computer I use for my NMR assignment, but I'm having problems to open the spectrum in the other computer. The message is that it can't find the directory. How do I solve this problem? I mean, how can I export the analysis I have done to another computer and continue my analysis there?

    • @SpectralSolutions999
      @SpectralSolutions999 2 года назад

      You need to copy all the source files (NMR data and project files (.cara)) to a new computer. Then you have to give new directory where your NMR data is located or set your working directory.

    • @cyntiaalves1819
      @cyntiaalves1819 2 года назад

      @@SpectralSolutions999 Thank you for your fast answer. I don't have experience with that computer stuff... How can I "give new directory" for my NMR data?

    • @cyntiaalves1819
      @cyntiaalves1819 2 года назад

      I think that would be a good idea for a new video about CARA NMR operation.

  • @ashiagrawal1719
    @ashiagrawal1719 2 года назад

    Thank you so much, you have saved me from a lot of trouble!

  • @nursyahirahkamal5556
    @nursyahirahkamal5556 2 года назад

    how to rearrange the graph in data list?

  • @harashkumarvt2013
    @harashkumarvt2013 2 года назад

    How to overlay NMR using MestReNova

  • @jibuttu
    @jibuttu 2 года назад

    I don't understand what you are doing.

  • @kishorekumar-gq4iu
    @kishorekumar-gq4iu 2 года назад

    how to open a vnmr file in top spin

  • @jibuttu
    @jibuttu 2 года назад

    Contact please

  • @deepaktripathi8428
    @deepaktripathi8428 2 года назад

    Very helpful.... Thank you🙏

  • @ganesh2gig
    @ganesh2gig 2 года назад

    I do not get the GUI feature as shown 1:05

  • @shongangai4513
    @shongangai4513 2 года назад

    Really Helpful. Thanks!!!!

  • @arpisworld4735
    @arpisworld4735 2 года назад

    With windows 11 which top spin version will be valid?

  • @rams3279
    @rams3279 3 года назад

    Thank you for your nice explanation. How could we add n number of spectra while comparing...for example some 11 spectra....since I was not able to compare more than 10..and topspin shows 10 instead of 11 while plotting

  • @arthuritodansledozo8204
    @arthuritodansledozo8204 3 года назад

    c est quoi ce bordel

  • @rahulhazari9212
    @rahulhazari9212 3 года назад

    Thank you so so much. I got my assignments ready because of your video. Keep going.