Видео

Thanks for the comments. For ImageJ or FiJi, AVI format is preferred.

Please read the description. Here is it. CORRECTION: In the video, Time Interval is the amount of time between two frames. I made a mistake by using the total clip time.

Unfortunately not, because it is a manual tracking method so you can track only one object at a time.

Thanks for the correction. Time Interval is the amount of time between adjacent frames. ‘x/y calibration’ is the number of your unit distance per pixel (the inverse of the number of pixels per unit distance).

Average will give you wrong numbers. The tracking is frame-by-frame, and for a total 50 frames you will have yo sum it, not average.

@@1400740 I don't get the point! I mean, how can the overall velocity during a path be the sum of the instantaneous velocities during that path? Help me understanding pls 😂 if I walk 1 seconf at 1m/s and then another 1 second at 2m/s my overall velocity is not 3 m/s

I was having the same question in mind... I'm sure Safdar has his point of view, but I'm sorry I couldn't understand it as well, which makes me stick with Simone's doubt... if the given value is already velocity taken from two frames... shouldn't we get an average value of those velocities for given range of frames? I hope you can clarify this doubt for us Safdar.. Thanks a million!

Hi Simone, recently I have verified that the sum of the velocities in the column is the average velocity of the given frames, Safdar is right, it can be easily proved by adding the total distance travelled by the particle divided by the time used. Indeed, the value calculated is similar to the sum of velocities. The question of yours, "why not take the average?", can be explained by defining the velocities in the velocity column as [distance travelled by the particle between two frames (micrometer)/ total time used for the complete track from first frame to the last (s)]. That's why the sum of these velocities will be the total distance travelled by the particle divided by the total time used for complete tracking. Hope it answers your doubt and have a nice day!

It is because the time interval is here improperly defined. In order to compute the instantaneous velocity, you have to divide by the interval of time between two frame, not by the total duration of the film made of N+1 images. So the instantaneous velocity in this video are false by a factor of 1/N. This explains why when Sadfar sums the N+1 values he got for the velocity, he miraculously recovers the good value for the average velocity ...

I don't think you need to calibrate for Z axis. We don't track motion in 3D, right? The video is 2D and hence only X/Y calib is needed. In reality, the motor indeed moves in 3D but we simply cannot track the velocity in 3D using this plugin.

Thanks. If you are refering to the blue line, it is drawn by the software by connecting the coordinates you clicked while tracking the moving particle.

Total interval of the clip.

Hi, sorry but why should you load the table with "Load Previous Tracking File"? if you need to do the calculation, open the data file in excel.

@@1400740 just for continuing the tracking work with previous tracking data when I did not finish all of the tracking work at once. :)

@@sungshilkim Better you do the tracking in one go. You should use short clips (5-10s long). It shouldn't be a problem to finish it in one go.

@@1400740 Sorry. my image sequence has lots of particles and I just want to record all of the tracking information in one csv file. Thanks for you reply anyway!

@@sungshilkim In such a case, auto-tracing is a better option. It more complex but does track several objects simultaneously. I never used it though. Good luck

I am pretty sure that .MOV video format is not supported by FiJi for tracking purpose. If I may remember correctly, its the .AVI format that I used in this video.

@@1400740 thats what I thought but I also changed the format to .AVI and it still appears like its not supported :(

Fatima Lara Yes, because the .mov to .avi conversion does not work as the file is already compressed due to the mov format. You must use a camera that records the clips in .avi or other uncompressed formats.

Hi.. did you figure it out how to analysis.. if it is can you help me out

Hi, sorry for that. It was my first video that I created ever, so I had a lack of experience. Didn't pay much attention to the sound.

Hi. It is possible that the file that you are trying to open is compressed. Most mp4 files are compressed. Fiji can only read uncompressed (like .avi) files.

You can download a decoder. I had the same problem but found a way to open the videos. I have a video on how to fix that.

No additional plugins needed. Its already in default imageJ.

@@1400740 probably it's available in Fiji application because it's not there in the default ImageJ application

@@oyster2334 Yes, plz try Fiji.

I remember I have used in ImageJ as well. But Fiji is better.

@@1400740 okay thank you so much Sir

Hi, Thanks for the question. The x/y calibration refers to the “pixel size”, (precisely, pixel distance) for calculating a velocity for in µm/sec. Take a look at this article which may be helpful. forum.image.sc/t/tracking-cells-using-imagej/595

You have to see the zoom of your objective and the pixel size of your camera. For example : your camera has pixel size: 16µm, and you use 63 x obj. your image in 1 pixel = 16/63 µm.

Hi Aviseka, should be no problem imo.

Hey :) Thank you so much for your reply . I would request to teach me how to measure it . I do have the video with me. can you please help me out :) Regards Avi

it will be great if can let me know the possibilities.

@@acharyaaviseka85 Sorry, I just noticed your response now. Did you manage to do it? You may have to first track your particle during the contraction, and then during the relaxation. You will get two kinds of velocities in this manner. I have never done such thing so I can't help much.

Peter Stubbs I found a great profile for the warthog stick and throttle. The trim works great with the same hat you referred to. I'll try to find the link for ya.

Cheers, I would appreciate it.

Any joy on that profile?

Ahh sorry man. I was having a hard time finding where I got it. I'm 99% certain it's from this link. drive.google.com/file/d/0Bxn37IgsfpTeekdsWFdyVGl6dUE/view?usp=sharing

Peter Stubbs let me know how that works for ya!

Absolutely. I used to struggle a lot with it before figuring out that it really needs a lot of trimming. Thanks for watching. :)

Absolutely

Safdar orbx trees?

It is fully compatible with orbx trees.

Safdar OK cool

grillodon Not really. At least not due to Terra flora.

Safdar ok. Thanks.

The Orbx trees tend to look darker. Terra flora gives you a lighter and brighter look as you see in this video. Also, Terra flora looks is more sharp.

Safdar Thanks! And performance-wise? Comparing with ORBX?

Thanks for comment. I don't see any noticeable FPS hit. I had most of the parameters set to max. You see that the fps remain above 50 all the time in this helicopter.

A comnination or noise and nice? :D