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Below are references and links to firther information about rMMS: Instructions card to make seed stock (pdf) www.douglas.co.uk/f_ftp1/rMMS_Procedure.pdf 'An automated microseed matrix-screening method for protein crystallization.' Acta D D'arcy et al. doi.org/10.1107/S0907444907007652 'Promoting crystallization of antibody-antigen complexes via microseed matrix screening.' Acta D Obmolova, Mali...

For Oryx8 XStep optimization click here: ruclips.net/video/0WvR3bHzgi8/видео.html The pH vs [precipitant] experiment can be used to create 24, 48 or 96 well optimization experiments with up to 5 drops per well.  Dispense precipitant gradients to the reservoir well with the 1 mL tip.  Dispense the drop using a 3 or 4 channel microtip.  Up to 5 drops per well. Control each drop independently. ...

Set up sitting drop and microbatch under oil experiments at the same time. The multi parameter experiment allows drop ratios to be varied and additives (e.g. seed stock) to be added. www.douglas.co.uk/

Microbatch Under Oil - 2D Grid Simple gradient optimization under oil. Create phase diagrams varying protein concentration and precipitant concentration. For more powerful optimization see XStep for Oryx8 www.douglas.co.uk/oryx8.htm#Autodesign

Manual slide mixer for preparation of Lipidic Cubic Phase (LCP) Method adapted from: Caffrey, M., Cherezov, V. Crystallising membrane proteins in lipidic mesophases. Nat. Protoc. 4, 706-731 (2009). Caffrey, M., Porter, C. Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases. J. Vis. Exp. (45), e1712, doi:10.3791/1712 (2010). Video of Method: www.jove.com/video/17...

www.douglas.co.uk/documents/oryx_lcp.pdf - Oryx LCP specifications / information. Dispense lipidic cubic phase from 10 nL to 1 uL to any plate for screening and optimization.

Dispense up to 5 drops on 22 mm cover slides for Hanging Drop experiments

www.douglas.co.uk/oryx8.htm#Autodesign Vapour diffusion optimization with XStep. [PEG 3350] vs pH. Using Cryschem M Plate (round reagent reservoir)

www.douglas.co.uk/oryx8.htm#rMMS A short introduction to random Microseed Matrix Screening (rMMS) Obmolova et al, Acta Cryst(2010) D66, 927 - 933: scripts.iucr.org/cgi-bin/paper?bw5361 D'Arcy at al. Acta Cryst (2007) D63: scripts.iucr.org/cgi-bin/paper?S0907444907007652 Shaw Stewart et al. Crystal Growth & Design - Random Microseeding: A Theoretical and Practical Exploration of Seed Stability a...