- Видео 51
- Просмотров 144 826
Susan Bush
Добавлен 16 сен 2011
BIOL307 Metabolism Refresher
This video is designed as a refresher on thermodynamics & an introduction as we head into glucose metabolism!
Просмотров: 79
Видео
BIOL307 Digestion of Fats, Sugars, and Nucleic Acids
Просмотров 853 года назад
This video is the second video on digestion (after the protein digestion video, found here: ruclips.net/video/-d9eEG48uaQ/видео.html). We focus on digestion of saccharides and triacylglycerides.
BIOL307 Protein Digestion
Просмотров 453 года назад
This video is about digestion of proteins; it is the first of two videos on digestion for BIOL307 Biochem! The second digestion video on carbs, fats, and nucleic acids is here: ruclips.net/video/dXkWfhUHRfs/видео.html
Regulation of the lac operon by Glucose
Просмотров 3323 года назад
BIOL304: Molecular Biology This video discusses the CAP protein that regulates the lac operon. Check out the other lac operon videos here: Intro to lac operon: ruclips.net/video/oM2l8MtrD9o/видео.html Lac operon problems ruclips.net/video/QCpABntmfOI/видео.html
BIOL307 Excel walk-through for buffer analysis
Просмотров 4743 года назад
This video takes you through the steps to summarize and visualize your buffer data from BIOL307 Lab 1 (Fall 2021), using Excel to make tables, do calculations, and create a line graph.
Intro to Allosteric Enzymes
Просмотров 1233 года назад
This video was created for BIOL307 to introduce the idea of allosterically regulated enzymes. We also compare the reaction curves of allosteric enzymes to those of Michaelis-Menten enzymes.
Calculations of fermentation - BIOL302
Просмотров 2,4 тыс.3 года назад
In this video, I walk through how to calculate how much fermentation your yeast cells have done, based on how much carbon dioxide is lost. I also talk about calculations based on your hemacytometer data. You should probably watch this at 1.5x speed.
Calculating LDH activity - BIOL307
Просмотров 10 тыс.3 года назад
In this video, I talk about how to calculate enzyme activity based on the change in absorbance, related to our LDH enzyme assay in BIOL307. You can probably watch this at 1.5x speed.
Catalase activity calcs for BIOL307!
Просмотров 1,8 тыс.3 года назад
In this video, I walk through how to calculate catalase activity based on the paper-disc-rising assay we used in BIOL307. Probably watch this at 1.5x speed.
Hormone signaling for Glycogen metabolism
Просмотров 1764 года назад
This video was created for BIOL307 Biochem to discuss the regulation of glycogen synthesis and breakdown by the hormones insulin and glucagon.
BIOL307: Gluconeogenesis
Просмотров 2704 года назад
This video was created for BIOL307: Biochemistry (Fall 2020). Enjoy!
BIOL304: Epigenetics & Agouti mice
Просмотров 6 тыс.4 года назад
This video was created for BIOL304: Molecular Biology (Fall 2020).
REs, plasmids, and E. coli growth
Просмотров 2784 года назад
In this video, I solve a problem about E. coli growth in the presence of antibiotics, given different restriction digests of the plasmid.
BIOL304 Restriction Enzyme Problem
Просмотров 4044 года назад
This video was created for BIOL304 to explain what's going on at the DNA level when a restriction enzyme digest is performed and followed by ligation of two DNA fragments together. Enjoy!
Genetic engineering in animals
Просмотров 1,9 тыс.4 года назад
This video was created for BIOL304: Molecular Biology. Make sure to also watch the video about GE plants here: ruclips.net/video/KNXOGCjdgFk/видео.html and ruclips.net/video/0lStCoX4rXQ/видео.html
How to make genetically engineered plants
Просмотров 19 тыс.4 года назад
How to make genetically engineered plants
Examples of genetically engineered plants
Просмотров 1,6 тыс.4 года назад
Examples of genetically engineered plants
BIOL307 Biochem: Using Henderson-Hasselbalch to make Buffers
Просмотров 1494 года назад
BIOL307 Biochem: Using Henderson-Hasselbalch to make Buffers
BIOL183: Solving Lac operon problems
Просмотров 34 тыс.4 года назад
BIOL183: Solving Lac operon problems
BIOL183: Control of Gene Expression - Operons
Просмотров 3004 года назад
BIOL183: Control of Gene Expression - Operons
BIOL183: Beadle & Tatum's One-Gene-One-Enzyme hypothesis
Просмотров 29 тыс.4 года назад
BIOL183: Beadle & Tatum's One-Gene-One-Enzyme hypothesis
BIOL183: Translation - Charging tRNAs
Просмотров 8974 года назад
BIOL183: Translation - Charging tRNAs
the conclution is even mutation will happen ( cut enzyme that NC can not produce protein cause mutation ) . so i choose , protein synthesis cause ariginin is protein but when i understand deeply . Splicing gen cause mutation and it can deactivation enzym produce each ornitin or ariginin .Sorry iam wrong.
I'm here an hour before exam
This is the best video ever you rock thank you
Where did the 1.5 ml come from at 7:35 ,
This is the total volume of reaction mixture in cuvvete
@Life-di2sv appreciate you 🤝
Very clear! Thank you!
Watching 2days before 3rd year final🤲🫶. Thank you miss
This is one of the best explainations I have listened to. Thank you.
Thank you for this video! The walkthrough of the practice problems were especially helpful!
You’re a great teacher.Thank you ma’am 🙏
Just for clarification: the BPA diet study on the mouse model, it doesn’t necessarily conclude if low methylation/high methylation is good/bad, right? The main conclusion is that, we see BPA diet can affect the methylation in mouse gene expression of the yellow fur coat gene - which means is just one example of how gene expression can be affected by something like BPA. Would that be correct ?
You are correct: this experiment tells us only that the diet and environment are able to influence genome methylation. The inference would be, the abundance of manmade chemicals and preservatives in our modern world might be having unpredicted (and unpredictable) effects on our genes and thus our health and development...and that of future generations.
is there a limit of gene size for t region?
Thanks
Awesome video just awesome
This was such a thorough breakdown of this experiment. I was having a hard time making sense of it from the textbook alone. Thank you!
Thank you 😊
you explain so so well wow finally
THIS WAS SO HELPFUL THANK YOU!!!!
Is it the samepathway
In.our book beadle and tatum.experiment lead to.biochemical pathway of niacin
so so helpful, thank you!!
Thank you Susan so helpful!
amazing🐁
i love you.
Thank you very much😍, my professor didn't teach the lactose operon mutation in university, but now I understand the topic completely after watching this video.
Hi Dr Bush, this is the best explanation so far. I d wish i had you as a teacher at university. The morons i had taught us nothing. Thank you.
Why does the methylation diet not impact the fur color of the mother but is passed on to the offspring?
I think it's because it is happening during the development of the mouse, not necessarily after the mouse has developed and grown.
why is it important to use haploid spores and not diploid of neurospora crassa in this experiement?
It's easier to make haploid mutants than diploid ones - you would only need the mutation in one copy (the only copy) of the gene.
I live in a suburb of Vancouver, Canada and the climate is mild enough for me to grow a "hardy orange" (Poncirus trifoliata) but no other type of citrus. From that youtube video, I can get a list of materials I will need. Where can I get the materials I need, plus the other knowledge necessary? I already have a blender.
DDT is organic and showing up in stores with the USDA Organic sticker. DDT was supposed to be globally banned. It's not.
Organic doesn't reduce chemical use. They still use synthetic pesticides. It's just a feel good bullshit label.
Very well explained! However, it’ll be nice to explain how the Chi-Square value was derived: perhaps in another video. Thanks!
Thank you for this amazing video! May I ask from which book the illustrations are taken from? I'd like to read more and I struggle to find materials about gene regulation problems such as these.
Hi Faissal, these images are primarily from Pierce Genetics published by Macmillan. Similar illustrations are in Campbell Biology, Alberts Cell Biology, and most other Cell/Molecular/Genetics texts.
Hi Susan thank you so much for your prompt reply, and thanks again for these great videos!
❤❤
2:15 Does that mean sweat potato is actually plant tumour ?
No, a sweet potato is actually part of the root system. It's for storing energy
Thanks!
Thank you so much! You explained so clearly!
👌🤌
god bless you
So helpful. Thanks
Thanks ♥️♥️♥️
thank you so much!!!! :)
Life saver! I wish my professors were at least half as good as you are
I don't understand where 0.52 and 0.08 come from. Would you not take the square root to solve for p and q?
0.52 and 0.08 are the expected homozygous genotype frequencies for RR and rr, given the current allele frequencies p & q. We calculate p-squared (freq of RR) by squaring p (allele freq of R) and q-squared (predicted freq of rr individuals) by squaring q (allele freq of r).
@@SciEdSusan I'm not sure what I'm missing here but something is just not computing for me :(
@@RuJoe720 There are a couple of questions we can ask using the HWE ideas. A common one is, if you know how many white (homozygous recessive rr) individuals there are in a population, then figure out p & q (the allele frequencies of R & r). In that case, usually you DO just take the square root of rr to get q! Here, we’re asking, given all three genotype frequencies, can we figure out if the population is in HWE? So first we calculate p & q, and since we are NOT assuming this pop is in HWE, we include the heterozygotes in the calculation. (We’re not assuming HWE bc we’re trying to test for HWE!) Then, once you have p & q, now you can calculate the expected or predicted values for each genotype, and compare them with what we already have observed in our population, to see if expected = observed. Hope this helps!
great explanation. Thank you.
This is super helpful. Thank you!
Very nice explanation maam. I have a question that is, I am taking total reaction volume of 250 ul. So while calculating, do i have to convert the value in ml that is.25ml (for example you have taken 1.5ml*1l/1000ml).likewise I have calculate in this manner. 25ml*1l/1000ml, so that ml gets cancel out and left out with mol unit.
That’s right! You need to cross-cancel to get to moles.
Thank u so much maam for your reply.
I was taking total reaction volume of 250 ul in calculation and was getting much higher relative enzyme unit. But after taking .25ml , the values I am getting is comparatively very less.
So will the cell survive if there is z-? Loss of function of LacZ?
Yes - as long as there's something other than lactose to eat!
Clear explanation that isn’t overly complex.
This helps a lot. THANK you so much!