- Видео 44
- Просмотров 239 589
Cube Biotech (Cube Biotech)
Германия
Добавлен 24 июн 2016
Cube Biotech is a privately held company based in Monheim, Germany. Cube Biotech was founded in 2012 by a group of experts in membrane protein biology and organic chemistry. With more than 40 years of experience shared among us, we provide: Excellence in membrane protein expression, purification, and crystallization. Expertise in synthesis of standard and tailor-made affinity matrices. High-quality products at reasonable pricing.
Next Gen P2X4 Antibody Discovery Enabled by NativeMP & Phage Display | Dr. Löwe & Dr. McCartin
Intelligent Infusions Masterclass: Next-generation P2X4 antibody discovery
Key Takeaways
• The unique challenges of P2X4: why existing antibodies fall short
• Revolutionizing Antibody Discovery with the NativeMP™ Platform and Phage Display Technology
• Discover how this technological breakthrough provides researchers with advanced tools to accelerate and enrich their own work
Speaker:
Maryna Löwe is a medical biotechnologist with a PhD in biochemistry and experience in drug discovery and development. She has a strong background in membrane protein research and is one of our project managers at Cube Biotech.
Conor McCartin is an account manager of the monoclonal antibody department at ProteoGenix.
Key Takeaways
• The unique challenges of P2X4: why existing antibodies fall short
• Revolutionizing Antibody Discovery with the NativeMP™ Platform and Phage Display Technology
• Discover how this technological breakthrough provides researchers with advanced tools to accelerate and enrich their own work
Speaker:
Maryna Löwe is a medical biotechnologist with a PhD in biochemistry and experience in drug discovery and development. She has a strong background in membrane protein research and is one of our project managers at Cube Biotech.
Conor McCartin is an account manager of the monoclonal antibody department at ProteoGenix.
Просмотров: 59
Видео
How do you choose a copolymer for solubilising your membrane protein target? With Prof. Tim Dafforn
Просмотров 1022 месяца назад
Intelligent Infusions Masterclass on the topic of copolymer screening for solubilizing membrane proteins effectively. Key takeaways: - Finding the correct protein for solubilization is important for Protein activity and yield - Copolymer screening is an efficient approach to copolymer selection - Affinity resins must also be selected carefully to maximize target yield Speaker: Tim Dafforn is Pr...
Cryo-EM structures of cell-free synthesized GPCR/G protein complexes with Dr. Bernhard
Просмотров 923 месяца назад
KEY TAKEAWAYS Cell-free expression is a modular system and protocols can be adjusted to different targets. E. coli lysates represent an affordable and efficient production pipeline giving fast access to non-truncated GPCR structures in active conformation. Co-translational insertion of membrane proteins into preformed nanoparticles avoids any detergent contacts and reduces sample preparation pr...
"Peptide-Protein Conjugation" with Andrew Lees, the founder and CEO of Fina Biosolutions
Просмотров 2103 месяца назад
Key Takeaways of the Masterclass: 1. Peptides and other haptens need to be chemically linked to a “carrier” protein for immunogenicity 2. Peptide solubility is one of the main challenges 3. Thio-ether chemistry is a simple conjugation approach but there are a lot of tricks for success 4. CRM197 (genetically detoxified diphtheria toxin) is widely used as a carrier protein with a few quirks 5. Re...
Structural insights into gating mechanism of GLIC in lipid environment with Dr. Nikhil Bharambe
Просмотров 1565 месяцев назад
Intelligent Infusions Masterclass on the topics of state-dependent protein-lipid interactions, offering a deeper molecular understanding of GLIC. Research Focus: Dr. Nikhil Bharambe is a Senior Research Fellow in the Basak Laboratory at the School of Biological Sciences, Nanyang Technological University (NTU) in Singapore. He began his career as an assistant professor at Modern College in Pune,...
Structure of Mechanosensitive Channel MscS in Native Lipid Nanodiscs with Elissa Moller
Просмотров 1615 месяцев назад
Intelligent Infusions Masterclass on the topics of Mechanosensitive Channels, Protein-Lipid Interactions, and Cryo-EM. RESEARCH FOCUS: Elissa Moller is a biophysics PhD candidate co-advised by Dr. Doreen Matthies in the Unit on Structural Biology at the National Institutes of Health and Dr. Sergei Sukharev in the Institute for Physical Science and Technology at University of Maryland studying t...
3 reasons why that protein structure may be closer than you think with Dr. Alex Snow
Просмотров 1566 месяцев назад
Intelligent Infusions Masterclass on the topics of Cell-Free Protein Expression, Copolymers, and CryoEM. RESEARCH FOCUS: Dr. Alexander JD Snow is a postdoctoral researcher working with Professor Alexander Breeze and Dr. Stephen Muench at the Astbury Biostructure Laboratory, University of Leeds. For the last two years he has focussed on characterising the receptor tyrosine kinase, FGFR3, through...
A quick and simple self-removing affinity tag with Prof. David Wood
Просмотров 1967 месяцев назад
David Wood is a professor of Chemical Engineering at Ohio State University and is a scientific co-founder at Protein Capture Science. David completed a double major in Biology and Chemical Engineering at Caltech, and then worked at Amgen on the Neupogen process in the early 1990’s. He completed a Ph.D. in Chemical Engineering at Rensselaer Polytechnic Institute, and worked briefly at Bristol Me...
The Role of Hemodynamic Forces in Zebrafish Heart Valve Formation with Dr. Renee Chow
Просмотров 577 месяцев назад
Renee Chow completed her PhD at the University of Cambridge, where she studied retinal development under the supervision of Prof. Bill Harris. She then joined the lab of Dr. Julien Vermot in Strasbourg, where she received a fellowship from the Lefoulon-Delalande Foundation and researched the role of mechanical forces in heart development. In 2023, she started her own lab at the Australian Regen...
Custom functionalized noble metal particles with Dr. Michael Erkelenz
Просмотров 948 месяцев назад
Intelligent Infusions Masterclass Series Key Points: • Functionalized particles have played an integral role across diverse sectors, spanning from pioneering scientific endeavors to large-scale biotechnological production. These particles encompass a spectrum of base materials, ranging from agarose to noble metals, each endowed with distinct physicochemical properties tailored to specific appli...
SMALPs as a platform to study bacterial membrane proteins in a native-like environment by Greg Dodge
Просмотров 5559 месяцев назад
Greg Dodge began his PhD studies at the University of Michigan, where he studied dehydratase domains from prokaryotic polyketide synthase and fatty acid synthase using X-ray crystallography. In 2018, he joined the laboratory of Prof. Barbara Imperiali at MIT, where he began his studies of phosphoglycosyl transferases from glycoconjugate assembly pathways in human pathogens. Here are some of the...
Lipid Bilayer Perturbations in Membrane Nanoparticles: an Intelligent Infusions Masterclass
Просмотров 18411 месяцев назад
Luis Real Hernandez is currently developing methods to determine the preferred surrounding lipids of membrane proteins using copolymeric nanodiscs. Key take-aways from his class: Copolymer solubilization of membranes directly extracts membrane lipids into membrane nanoparticles While preserving membrane protein function in membrane nanoparticles is important, so is preserving native membrane li...
Characterization of Macrophage Phenotypic States and Cellular Responses to Nanomedicines
Просмотров 28311 месяцев назад
Jonas Bossart's research is focused on the quantitative proteomics of human immune cells and aims to define their roles in disease development and treatment responses. He is currently investigating macrophages' phenotypic landscape and their response to widely used iron-carbohydrate complex nanomedicines. Key takeaways • Pro-inflammatory phenotypes show high activity of p38, JNK, and JAK kinase...
Synthetic Copolymer Nanodisc Enabling Near-Native Membrane Protein Assays with Dr. Merino
Просмотров 382Год назад
This masterclass is about the largest proteome-scale investigation of membrane protein solubilization to date. The class is based on research conducted by Dr. Merino and scientists from the University of Cologne. You'll learn how copolymers can solubilize and stabilize membrane proteins without the need for traditional detergent-based methods and enhance your understanding of near-native membra...
Building artificial cell membranes with Dr Samar Damiati an Intelligent Infusions Masterclass
Просмотров 397Год назад
Building artificial cell membranes with Dr Samar Damiati an Intelligent Infusions Masterclass
Unlock protein structures with Cube Biotech’s cryo-EM services
Просмотров 7222 года назад
Unlock protein structures with Cube Biotech’s cryo-EM services
How to make MSP nanodiscs: The protocol
Просмотров 3,7 тыс.3 года назад
How to make MSP nanodiscs: The protocol
Membrane protein solubilization & stabilization: The purpose of nanodiscs
Просмотров 5 тыс.3 года назад
Membrane protein solubilization & stabilization: The purpose of nanodiscs
How to purify proteins with a drip columns / column chromatography.
Просмотров 9 тыс.3 года назад
How to purify proteins with a drip columns / column chromatography.
"Recent development on SMALPs" (Jan Kubicek) - SMALP Meeting February 2021 -
Просмотров 5173 года назад
"Recent development on SMALPs" (Jan Kubicek) - SMALP Meeting February 2021 -
How to do FPLC - an example of affinity chromatography
Просмотров 34 тыс.3 года назад
How to do FPLC - an example of affinity chromatography
How to purify Proteins using Batch Spin
Просмотров 7 тыс.3 года назад
How to purify Proteins using Batch Spin
Affinity chromatography: How to pack columns / cartridges
Просмотров 10 тыс.4 года назад
Affinity chromatography: How to pack columns / cartridges
SMALP: How to solubilize membrane proteins using synthetic nanodiscs | Cube Biotech
Просмотров 3,4 тыс.4 года назад
SMALP: How to solubilize membrane proteins using synthetic nanodiscs | Cube Biotech
"How Cube Biotech helps to fight COVID-19" (Jan Kubicek) - German Corona Showcase 2020 -
Просмотров 3894 года назад
"How Cube Biotech helps to fight COVID-19" (Jan Kubicek) - German Corona Showcase 2020 -
"How to do E-Commerce in a small biotech" (Michael Ries) - BioTec-Meeting: Digital Unterwegs 2019 -
Просмотров 2975 лет назад
"How to do E-Commerce in a small biotech" (Michael Ries) - BioTec-Meeting: Digital Unterwegs 2019 -
Membrane protein services @Cube Biotech
Просмотров 1,6 тыс.5 лет назад
Membrane protein services @Cube Biotech
Came across this video and found it very interesting. I have a defect in the trpm7 gene and my health has been declining steadily over the past 15 years. Very scary. My Drs have no information and I’m afraid this may eventually be the cause of my death. Anyone out there know who I can contact to help?
🥹🙏🏻❤️
Thanks really needed someone to explain this topic clearly.
A cute animation that does a good job of describing what is happening :)
nice job but i hAve final exam after 5 hour clinecal bio chem 🤯🤯💀☠☠
Same, after 3 hrs
I am working in a completely irrelevant field and this video was so good that is was possible for me to understand everything. Thank you!
Thank you for the kind words! I'm glad to hear that the video made such a complex topic accessible, even for those outside the field. We strive to make our content clear and engaging for everyone, regardless of their background. :)
:)
Thank you! - A biomed student in biochem
This is really well made. Great job
Thank you very much :)
Very helpful!! Thanks so much!!😘
You are welcome :)
Great video! Are these magnetic beads reusable for multiple purifications or are they single use?
Our standardbeads are all resuseable. He have regeneration protocols for all of them :)
@@CubeBiotech thanks :)
can someone explain me the affinity and specificity logic
It is a trade-off. Higher affinity means higher vield, but that usually comes with less purity/specificity. Because if you make it easier to bind, false targets also get a chance to bind. And in the other direction this applies as well. If you make it harder to bind - higher specificity, you get purer results but also less yield.
Amazing 🎉
Thank you :)
such a cute video and great explanation!! thank you <3
Thank you :)
Super cute and helpfull, thank you! ❤
Thank you :)
In native page , sds is not used , and proteins are. Not separated by charge only , but also by their Size and Shape ,. SDS an anionic detergent Provides The same negative charge per unit of Protein and Degenerates Its Complex Structure Into Linear One
Thank you for providing these additional information :)
This is fantastic
Thank you :)
such a cool and interesting video! NEVER KNEW THIS!
Glad that we could help you there. Did you mean something specifically?
@@CubeBiotech just the whole process in general! I had to do an oral presentation and this was one of the main topics!
This shit goes hard 👍👍👍
Thank you :)
Well simplified. Thank you for the good work.
Hi Patrick, thank you for the positive feedback! That is always appreciated :)
I didnt look up a vid to have to read...
We are sorry to hear that. All videos after this one are narrated. We hope you still found the information provided here useful.
This is the most useful video I ve seen on this topic so far
Thank you very much. We are really glad to hear that!
now say the chemical composition of titin
Hello r u life science student
No we are a biotech company :)
thank you
You are welcome :)
Thank you for such an amazing demonstration. Please can you help and make demonstration on periplasmic protein purification in E. Coli. I think this demonstration is not available on YT.
We are glad that we could help you. These videos take time to make, and we currently have a longer list we like to work on first. But you recommendation is considered :)
03:38 precipitation method, salting out
Time stamps are set :)
C169719H270466N45688O52238S911
SDS PAGE plz
Noted and thank you :)
studying for my MCAT thank you!
Good Luck !
Sticky ball😂😂
A very annoying sticky ball.
can you send me the sides
There are no slides, sadly. These are, for the most part, Adobe After Effects files. :(
Many many thanks for this explanation ❤
We are happy it helped you. What part of the video did you find the most helpful?
So helpful!
Thank you :)
Exactly 😂
Merhaba ben Türkiye'den bir aşı üretim kululuşunda çalışmaktayım.viral inaktif aşı üretmekteyiz.orotein konsantrasyon saflaştırma filtrasyonu (PEG) ve Kromotografi yöntemleriyle çalışmaktayız.sizinle irtibata geçmek istiyorum.
(Google Çeviri ile yazılmıştır) Teşekkür ederim. Yapılacak en iyi şey bize “Contact@cube-biotech.com” adresine bir e-posta yazmaktır.
Teşekkürler posta biraktim
Vortexing protein?? 😱
Be assured. This is the correct protocol for handling our SMALP products. :)
Wowww I am beyond amazed. This lesson was explained to us in 2 hours and was so tiring and confusing but you managed to clear it all out in 13 minutes. So grateful 🙏🙏 definitely recommending this channel to my classmates❤
Thank you very much for these kind words! That definetly made the day of the lead creator of this video! Thank you for further recommending us!
Thanks a lot ❤
You are welcome :)
Nice video. It clearly described FPLC in a very simple way and to the point. Thank you!
Thank you :) We hope it well help you in the future.
now pronounce the 12th isoform of it
Ask the guy who made that video :P
Very Good and useful.thanks for describing so deeply
Glad that we could help :)
Great video! Love the animation so muchh!
Thank you very much as well :) Glad to see that the effort pays off.😊
Can I use one step purification FPLC?
Hello :) You mean if just a single purification method is enough? His-tag purifications always leave some impurities behind. That is the trade-off for its incredible protein yield. In-house we usually follow-up with a Size-Exclusion chromatography if the His-tag purification was not enough.
I bet you’re from india
I bet u are from fbi
Nope. Germany :)
I do this In my lab with his-tag for bacterial pesticidal proteins. this explanation helps clear things up. if im not mistaken the reason we conduct dialysis buffer exchange after is to remove any excess imidazole from our target protein as it is toxic to the insects we test and may interfere.
Glad that we could help. And yes this sounds reasonable. Normal Imidazole concentrations in His-tag elution buffers is 500µM. This almost certainly must be washed away first through dialysis for any downstream animal tests.
This video is amazing and really helpful! Thanks :)
Really Glad to hear that. It makes us happy that the effort was worth it!
@@CubeBiotech 💜
Hello from California! I have a question. Do Golgi in all types of cells (liver and kidney cells) have the same function and structure ? If we were to purify the Golgi ( fractionation) from different cells, (not plant) would they function the same and have the same structure ??? I can’t seem to grasp this concept.
Good Day :) We are experts on protein purification and not the Golgi-Apperatus. We do not feel comfortable to answer this for you. What we do recommend to ask this question on platforms like Researchgate. You should find your answer there.
Thank you so much for your guidance!!!
I love it <3
We are glad that you like it :)