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OiVM - Optical Imaging & Vital Microscopy
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Добавлен 27 июл 2020
Optical Imaging & Vital Microscopy (OiVM) Core Facility at the Baylor College of Medicine
We are a Light Microscopy Core Facility Specializing in Optical Sectioning Tools for Fluorescence Microscopy
The mission of the Optical Imaging & Vital Microscopy (OiVM) Core is to give our investigators the tools and technical expertise they need to obtain reliable, reproducible scientific data using state-of-the-art microscopy modalities. We have over 20 years of experience educating, training and assisting scientists with experiments using the latest cutting edge imaging methodologies.
Our core specializes in vital and intravital imaging of cellular processes within cells, intact tissue explants, developing embryos and functioning organs within the live animal.
We are a Light Microscopy Core Facility Specializing in Optical Sectioning Tools for Fluorescence Microscopy
The mission of the Optical Imaging & Vital Microscopy (OiVM) Core is to give our investigators the tools and technical expertise they need to obtain reliable, reproducible scientific data using state-of-the-art microscopy modalities. We have over 20 years of experience educating, training and assisting scientists with experiments using the latest cutting edge imaging methodologies.
Our core specializes in vital and intravital imaging of cellular processes within cells, intact tissue explants, developing embryos and functioning organs within the live animal.
Training: 3D Image Reconstruction with NRecon
A descriptive software guide for reconstructing three dimensional (3D) data sets from X-Ray back projection images scanned on the Bruker Skyscan 1272 microCT.
For training on how to collect images for reconstruction, please see our full training video for the Bruker Skyscan 1272 microCT scanner here: ruclips.net/video/v9HZNvlzLCw/видео.html
00:00:00 - Introduction
00:00:36 - Starting NRecon Software
00:01:39 - Layout of NRecon
00:02:40 - Loading Dataset into NRecon
00:07:35 - Optimize the Reconstruction Parameters
00:08:11 - Misalignment Compensation
00:10:19 - Smoothing
00:11:59 - Object Larger than the Field of View
00:12:39 - Ring Artifacts Reduction
00:13:41 - Beam-Hardening Correction
00:14:33 -...
For training on how to collect images for reconstruction, please see our full training video for the Bruker Skyscan 1272 microCT scanner here: ruclips.net/video/v9HZNvlzLCw/видео.html
00:00:00 - Introduction
00:00:36 - Starting NRecon Software
00:01:39 - Layout of NRecon
00:02:40 - Loading Dataset into NRecon
00:07:35 - Optimize the Reconstruction Parameters
00:08:11 - Misalignment Compensation
00:10:19 - Smoothing
00:11:59 - Object Larger than the Field of View
00:12:39 - Ring Artifacts Reduction
00:13:41 - Beam-Hardening Correction
00:14:33 -...
Просмотров: 3 223
Видео
Training: Bruker Skyscan 1272 microCT
Просмотров 4,6 тыс.3 года назад
A complete user training for acquisition of shadow projection images for 3D XRay microCT reconstruction using a Bruker Skyscan 1272. For the reconstruction of back projection images to 3D slices - please see our NRecon training video here: ruclips.net/video/9k8et7L62Bs/видео.html 00:00:00 - Introduction 00:00:37 - Overview of the Skyscan 1272 00:01:22 - Power on the Skyscan 1272 00:02:50 - Agin...
Using Immersion Objectives
Просмотров 4,1 тыс.3 года назад
An instructional video on using immersion objectives. We discuss why it is necessary to use immersion optics as well as how to properly use them on inverted microscopes. 00:00:00 - Introduction 00:00:25 - Why Do We Need Immersion? 00:05:56 - How to Use Immersion Fluid 00:15:05 - Positioning The Stage 00:16:59 - Changing Specimens 00:18:10 - Cleaning Up
Training: Advanced Airyscan Processing
Просмотров 3,5 тыс.3 года назад
An advanced discussion of strategies for improving the processing of Airyscan imaging data. This supplementary training offers additional recommendations on the secondary processing stages for 2D and 3D data sets. Before viewing this video, please see our Airyscan & AiryscanFAST training video here: ruclips.net/video/dBmPAvI1_ZQ/видео.html 00:00 - Introduction 01:51 - Processing 3D Images 07:39...
Training: Airyscan & AiryscanFAST
Просмотров 14 тыс.3 года назад
A comprehensive training module for the Airyscan & AiryscanFAST detection technologies of the Carl Zeiss LSM 880 confocal microscope. This training discusses the basic theory of Airyscan and AiryscanFAST imaging and provides a detailed discussion of how to use each mode for your experiments. The concepts discussed here rely on knowledge of the LSM 880 confocal microscope - and we recommend that...
Training: Carl Zeiss LSM 880 Confocal Microscope
Просмотров 34 тыс.3 года назад
Please watch our Introduction to Confocal Microscopy lecture before taking this training: ruclips.net/video/vn6app6XuII/видео.html A complete user training for the Carl Zeiss LSM 880 confocal microscope. This training takes the user from powering the instrument on through collecting 3 color confocal images with 3D Z-Stacks. This comprehensive training video is designed for Baylor College of Med...
Introduction to Confocal Microscopy
Просмотров 49 тыс.3 года назад
An introduction lecture on the basics of confocal microscopy. In this video you will learn about how fluorescence works, how a confocal image is formed and discuss the advantages and disadvantages of using this technology. This video is a prerequisite for training on any of the OiVM core's confocal microscopes.
Very nicely explained. Both the physics and biology of the system
Best ever explanation I have even watched on RUclips so far.
Great tutorial! very knowledgeable and in details
Good stuff. Thanks a lot!
thanks!
Hi can you suggest some deconvolution software that u use? I am going to work with both cell culture and tissue sections
Check out Scientific Volume Imaging here: svi.nl/HomePage
such an excellent resource !
This video is PERFECT
well explained
I am totally cleared after seeing the tutorial.
Thanks , it was worthy .
Easy to follow explanation with excellent graphics.
Thanks for great sharing!.
noice.
Toit
Best coverage on the confocal microscope 👏👏👏👏👏👏👏👏👏
Thank you for your clear explanation.
I have a doubt.is it possible to ct scan a object which has zero transmission to X ray?.
Where did you get the "skirts" for the objectives?
Zeiss makes this - it's called the Aqua Stop II.
Hi Thank you so much for making that video its amazing and the voice is very comfortable to listen to
Can you share how to change and keep the CO2 chamber for live cell imaging
By far the best introduction to confocal microscopy I have ever seen on youtube
Excellent!!!!!
The best.
Amazing Informative go ahead thank you brother I have subscribed to the channel.
Thank You so much. You are a live saver. I don't understand Y people watch the video and don't subscribe.😖😞
I've been looking for a tutorial that explains both the "how" AND the "why", and this absolutely did both! Fantastic video, thanks for posting!
Great video, Thank you so much.
How about taking DIC or PC please, Thanks
Excellent video! Very nicely explained
Thank you for the great tutorial! It helped me so much.
Awesome! Glad it was useful for you.
Thanks for helping me understand the Airyscan principle.
The greatest training ever! Thanks
Thanks a lot! That clarified many questions I had after watching other tutorials on the same topic.
Can't say enough how well you made this.. Thanks a million 💜💜 Keep it up 👏✅
Very good explanation. Its appreciate able ..thanks 👍
This tutorial is so great! Do you have a written protocol that I can print so all the students can follow? We use a shared equipment and all the settings are deleted when we exit the program. Is there a way to get the same settings when we go back to the confocal? Thank you s much!
Thank you Cleusa! We do have a PDF guide on our website: oivm.org/lsm880/#training As for saving your settings - you can either save them to the Experiment Manager at the top of the Acquisition dialog or you can load them from an image by clicking the 'Reuse' button from any CZI image taken with the same system.
@@OiVM Thank you Jason. The guide has some areas where the text is not very visible. I looked for the "reuse" button but I couldn't find it. Is it possible that we don't see it because of the version that we have?
@@cleusaoliveira9788 You can find the Reuse button in the 'Dimensions' tab located at the bottom of the image display.
Thanks!
Thanks so much for your training video! I almost forgot how to perform the scanning when I need to scan sth. Have a nice day.
I Appreciate your efforts.
Masterful presentation!
Thank you so much❤️
This is a great video! Thanks for making it! :)
Thank you for this helpful video! I have a question concerning the Beampath Configuration: How can I change the Detectors? Instead of Ch1, CHS1 and CH2 I can only choose between CH1, CH2 GaAsP and CH3. How can I set up the same detectors you showed in this video? I'd be extremely thankful if you could help me out with that!
Glad you liked it! Detector choice will depend on your system configuration. Some LSM 780/880 instruments are equipped with a single PMT instead of the 32 channel spectral array (ChS) in that second detector slot. Regardless, the order of the channels would be the same for the sequential acquisition presented. On your system use Ch2 GaAsP in place of ChS1 and Ch3 in place of Ch2. Ch1 remains the same. You will just rely on the upstream prisms for emission separation to Ch2 rather than the electronic selection the 32 element spectral array provides.
The video quality is superb! You should upload more videos!
Another nice sharing. Thank you so much!
Thanks so much!
Very clear and concise, thank you!
The best !!!!
Thank you for sharing. Very useful.
BCM is amazing! Thanks for this video!