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- Просмотров 75 875
Microscopy Services Laboratory UNC
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Добавлен 2 июн 2020
Microscopy lectures and tutorial videos from the Microscopy Services Laboratory at the University of North Carolina at Chapel Hill
APPENDIX Other macros you may find useful (HD)
Adjust RUclips settings to HD to see FIJI menus and commands.
Materials for exercises available here:
doi.org/10.17615/d372-5m24
Materials for exercises available here:
doi.org/10.17615/d372-5m24
Просмотров: 17
Видео
OPTIONAL - Thresholding based on Romain Guiet method, tolerable number false + pixels (HD)
Просмотров 322 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
OPTIONAL - Data wrangling with multiple files (HD)
Просмотров 312 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
10 Run analysis on full 2D dataset using manual thresholds from paper (HD)
Просмотров 442 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
9 Using a single Z stack to explore thresholding options (HD)
Просмотров 312 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
8 Using multiple images to explore thresholding options (HD)
Просмотров 672 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
7 Using a single image to explore thresholding options (HD)
Просмотров 442 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
6 Measuring colocalization parameters (HD)
Просмотров 612 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
5 Visual check of data - 2D histograms with ROIs (HD)
Просмотров 502 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
4 Visual check of data - 2D histograms (HD)
Просмотров 572 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
3 Visual check of data - line profiles (HD)
Просмотров 522 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
2 Visual check of data - yellow (HD)
Просмотров 442 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands. Materials for exercises available here: doi.org/10.17615/d372-5m24
1 Data wrangling creating a subset of the data (HD)
Просмотров 692 месяца назад
Adjust RUclips settings to HD to see FIJI menus and commands Materials for exercises available here: doi.org/10.17615/d372-5m24
Intro to colocalization exercises
Просмотров 1002 месяца назад
List of everything you need before starting the colocalization exercises. Download materials here: doi.org/10.17615/d372-5m24
Colocalization lecture
Просмотров 5672 месяца назад
Presentation and materials for exercises available here: doi.org/10.17615/d372-5m24 What can colocalization refer to? (0:48) Need resolution to interpret colocalization (4:26) Nomeclature (7:56) Imaging and pre-processing decisions (9:10) Quick check - yellow or white (17:27) Quick check - line profile (20:11) Quick check - 2D histogram (22:21) Colocalization metrics (25:31) Intensity correlati...
3 LSM900 Shutdown - retraining for LSM700and710 users ONLY
Просмотров 238Год назад
3 LSM900 Shutdown - retraining for LSM700and710 users ONLY
24 Transmitted light imaging on the confocal (OPTIONAL)
Просмотров 356Год назад
24 Transmitted light imaging on the confocal (OPTIONAL)
23 Using Zen connect to relate multiple images taken in same sample (OPTIONAL)
Просмотров 633Год назад
23 Using Zen connect to relate multiple images taken in same sample (OPTIONAL)
22 Using the camera for fast mapping of the sample (OPTIONAL)
Просмотров 142Год назад
22 Using the camera for fast mapping of the sample (OPTIONAL)
18 Imaging at different positions (OPTIONAL)
Просмотров 203Год назад
18 Imaging at different positions (OPTIONAL)
17 Using preview images to navigate the sample
Просмотров 302Год назад
17 Using preview images to navigate the sample