- Видео 23
- Просмотров 41 703
NSERC PROMOTE McGill
Канада
Добавлен 11 мар 2021
Nanoswitch
This video gives a brief overview of how to determine the stability of a simple DNA nanoswitch (i.e., a DNA stem-loop) by measuring fluorescence changes at different urea concentrations. This technique was inspired by the classic protein urea denaturation experiment and was adapted to the analysis of DNA nanostructures by the lab of Vallée-Bélisle (Idili, A.; Ricci, F.; Vallée-Bélisle, A. Nucleic Acids Research, 45 (13), 2017, 7571-7580).
Leave a comment below, if you have questions.
Alexis Vallée-Belisle Lab
University of Montréal, Montréal, Canada
Leave a comment below, if you have questions.
Alexis Vallée-Belisle Lab
University of Montréal, Montréal, Canada
Просмотров: 8
Видео
Western-blot
Просмотров 1023 месяца назад
This video gives a step-by-step overview on how to do a western blot for protein separation and identification. Watch our video to learn more! 💡 McKeague Lab of Genomic Chemistry McGill University, Montréal, Canada
Introduction to Rheology
Просмотров 956 месяцев назад
This video will give you an overview of the field of rheology and its potential applications. You will also find a quick tutorial for using rotational rheometers, which allows the characterization of viscoelastic properties of a material (e.g., hydrogel, paste, etc.). Let us know if you have any questions. The Shoichet Lab University of Toronto, Ontario, Canada
Studying Oligonucleotide Duplex Stability Using Thermal Denaturation
Просмотров 1337 месяцев назад
This video demonstrates how to use a UV-Vis spectrophotometer. This instrument is an important tool for studying nucleic acid base pairing interactions, including folding into complex secondary structures and binding to a complementary target sequence. If you have any comments or questions, let us know below! Merry Christmas and Happy New Year 2024! 🎄❄️ Damha Lab McGill University, Montréal, Ca...
Photostabilizing Strategies for Single-Molecule Fluorescence Microscopy
Просмотров 31411 месяцев назад
A single-molecule fluorescence microscopy is a powerful tool for investigating biological entities but the good probes alone are usually far from enough to meet the experimental requirements. This video describes our strategy to improve the photostability of the probe by quenching the triplet excited states. If you have any comments or questions, let us know below! The Cosa Group McGill Univers...
Bio-layer interferometry
Просмотров 3,5 тыс.Год назад
Bio-layer interferometry is a label-free technology used to find the biomolecular interactions in real-time between two molecules of interest. This technique has significant applications in identifying properties of reaction kinetics, such as rate of association and rate of dissociation. Additionally, due to its ability to achieve real-time high-resolution data, BLI is useful in pharmaceutical ...
Flash Column Chromatography
Просмотров 9 тыс.Год назад
This video gives an introduction to the small molecule purification technique of flash column chromatography. It includes the selection and packing of a column with silica gel, loading of a compound onto the column and monitoring the elution of the desired species from the column. Contributions by PROMOTE Undergrads: Kirti Purkayastha and Elyse Moisan If you have any questions, do not hesitate ...
Docking: Molecular modelling computational chemistry.
Просмотров 324Год назад
In this video, we are going to go over the steps for docking using our lab’s docking software ‘Forecaster’. Docking is a computational method that is used to predict the binding site and the binding energy of a ligand to the macromolecule. Merry Christmas and Happy New Year 2023! 🎄✨ If you have a specific question or questions about how to use ‘Forecaster’, do not hesitate to ask in the comment...
NSERC PROMOTE presents An hour with Dr. Claudia Aloisi
Просмотров 43Год назад
It was our pleasure to host a networking event with Dr. Claudia Aloisi on November 10, 2022, where she shared her career path that spanned five countries! Also, Dr. Claudia talked about her research, career travels, mentoring, and advice. Thank you, Dr. Aloisi, for sharing your stories and experience.
Surface Preparation for Single-Molecule Fluorescence Studies
Просмотров 2242 года назад
This video gives a step-by-step protocol for PEG-Silane passivation of glass surfaces for single-molecule fluorescence studies. To find out more about our work in this video, please consult our recently published paper pubs.acs.org/doi/10.1021/acsami.8b15796 And do not hesitate to ask questions in the comments! ✍🏻 The Cosa Group McGill University, Montréal, Canada
Molecular Pendulum - a universal platform for reagentless biosensing
Просмотров 3212 года назад
This video gives a quick overview and a general workflow on the Molecular Pendulum (MP) - a nanoscale biosensing technology for universal, continuous biomarker monitoring developed in the Kelley Lab at the University of Toronto. It covers the fundamental working mechanism of MP, preparation and functionalization of the electronic chips to anchor MPs on gold nanostructured microelectrodes, elect...
RNA radiolabeling experiment
Просмотров 5872 года назад
In this video, we will show and explain how to radiolabel your RNA in the lab. If you have specific questions let us know and stay tuned! Functional nucleic acid research group of Prof. Yingfu Li McMaster University, Ontario, Canada
An hour with Dr. Jennifer Heemstra
Просмотров 1142 года назад
NSERC PROMOTE presents ''An hour with Dr. Jennifer Heemstra''. Jennifer Heemstra is a Professor of Chemistry at Emory University. Her research makes use of the ability of nucleic acids to self-assemble and recognize other molecules. Enjoy this one-hour networking event: career pathing, mentoring, questions session, stories.
PAGE image processing
Просмотров 1102 года назад
We mastered the skills in making and running PAGE gels. However, do you know how to visualize your data? This video will explore a detailed procedure of image processing using Amersham Typhoon gel visualizer. If you have any questions let us know! ✍🏻 Functional nucleic acid research group of Prof. Yingfu Li McMaster University, Ontario, Canada
Lateral Flow Assays
Просмотров 5 тыс.2 года назад
A Lateral flow assay (LFA) is a paper-based platform for the detection and quantification of analytes in complex mixtures. In this video, we give a rudimentary introduction to an aptamer-based lateral flow assay. Discussed are its components, uses, mechanism and production. If you have any questions, ask us below! ✅ Laboratory: The Laboratory for Aptamer Discovery and Development of Emerging Re...
Introduction to Polyacrylamide gel electrophoresis (PAGE) for Oligonucleotides
Просмотров 2,4 тыс.2 года назад
Introduction to Polyacrylamide gel electrophoresis (PAGE) for Oligonucleotides
How to make an agarose gel for electrophoresis
Просмотров 7173 года назад
How to make an agarose gel for electrophoresis
Interview with Melanie Brochu STEM (PROMOTE NSERC Mentor)
Просмотров 403 года назад
Interview with Melanie Brochu STEM (PROMOTE NSERC Mentor)
Basic introduction to DNA synthesis
Просмотров 2,9 тыс.3 года назад
Basic introduction to DNA synthesis
Basic introduction to Flow Cytometry
Просмотров 3,5 тыс.3 года назад
Basic introduction to Flow Cytometry
2:20 Pirify dna sequence to only extract desired sequence
why does your software look like it's running on windows 95?
Please, please, never write on the plate while voltage is applied. Either label the plate beforehand or turn off the voltage. Never handle a live plate.
Why is much of your content classified as “for children”? Comments cannot be posted on Childrens comtent and you cannot create a playlist. More importantly, children certainly what chromatography is.
Which voltage is appropriate for protein separaten gel
Can you please also teach SELEX steps by doing practical work
❤❤❤❤❤❤❤
hey, Thank for sharing . I have a question. Actually i want to separate oligonucleotides on PAGE . So for analysis If i am using UV shadow analysis . How can i do this with TLC plate?
Great, I’m trying to get a surface like you made also I’m following your article since last December. I’m happy to watch your videos more and more.
What was the concentration of resazurin
such a good vdemonstration
This video saves my life 🥲Thank you!!!!
Can you please please share the protocol of dephosphorylation and labeling of RNA... I cannot see anything on x-ray film..! I am from iit Kanpur, India
Love the video, very helpful.
Thank you for watching it!