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Dr. Asif's Mol. Biology
Япония
Добавлен 23 янв 2021
Hi, I am Dr. Asif Ali, a Molecular biologist by training and now working as a Postdoctoral Researcher.
This channel aims to build a platform for fresh graduates, who are just starting their research and facing problems in Biotechnology and Molecular Biology. I will cover different topics of scientific writing skills, advanced laboratory equipment tutorials, and training in bioinformatics tools and software.
If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also benefit from this discussion.
[🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑]
This channel aims to build a platform for fresh graduates, who are just starting their research and facing problems in Biotechnology and Molecular Biology. I will cover different topics of scientific writing skills, advanced laboratory equipment tutorials, and training in bioinformatics tools and software.
If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also benefit from this discussion.
[🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑]
AgriGO: A web-based tool to perform gene ontology enrichment analysis
#agrigo #geneontology #enrichmentanalysis
Просмотров: 353
Видео
Linux commands tutorial for beginners
Просмотров 12414 дней назад
Linux commands tutorial for beginners
How to create a Linux sub-system in Windows for running RNA_seq pipeline | Trimmomatic and HISAT
Просмотров 19314 дней назад
How to create a Linux sub-system in Windows for running RNA_seq pipeline | Trimmomatic and HISAT
How to download multiple accession data using the SRA tool kit
Просмотров 19621 день назад
How to download multiple accession data using the SRA tool kit
How to install and run FastQC in Windows and report interpretation
Просмотров 472Месяц назад
How to install and run FastQC in Windows and report interpretation
How to draw a heatmap with clustering using a web tool?
Просмотров 591Месяц назад
How to draw a heatmap with clustering using a web tool?
How to write a review of literature using Scispace and Copilot AI tools
Просмотров 253Месяц назад
How to write a review of literature using Scispace and Copilot AI tools
Use of modern AI tools to write a literature review
Просмотров 132Месяц назад
Use of modern AI tools to write a literature review
How to draw a circular gene ontology and KEGG plot?
Просмотров 1,1 тыс.Месяц назад
How to draw a circular gene ontology and KEGG plot?
An introduction to a web-based tool for free Bioinformatics plots | Chi Plot
Просмотров 1,1 тыс.Месяц назад
An introduction to a web-based tool for free Bioinformatics plots | Chi Plot
How to perform hierarchical clustering and co-expression networks in Genevestigator?
Просмотров 371Месяц назад
How to perform hierarchical clustering and co-expression networks in Genevestigator?
Disgenet | How to download a list of genes from gene-disease association database?
Просмотров 480Месяц назад
Disgenet | How to download a list of genes from gene-disease association database?
How to download sequencing data from SRA NCBI using windows SRA tool kit ?
Просмотров 1,1 тыс.2 месяца назад
How to download sequencing data from SRA NCBI using windows SRA tool kit ?
An introduction to first code in R | ggplot2 for plots and graphs
Просмотров 3652 месяца назад
An introduction to first code in R | ggplot2 for plots and graphs
How to load and view any stored dataset in R packages
Просмотров 1432 месяца назад
How to load and view any stored dataset in R packages
How to change language of messages in R console
Просмотров 1802 месяца назад
How to change language of messages in R console
How to install and remove packages in R Studio?
Просмотров 1672 месяца назад
How to install and remove packages in R Studio?
Getting started with R | Customizing layout in R studio
Просмотров 1812 месяца назад
Getting started with R | Customizing layout in R studio
Getting started with R | downloading and installing R and R Studio
Просмотров 1692 месяца назад
Getting started with R | downloading and installing R and R Studio
How to perform multiple sequence alignment without any software? | Mult Alin
Просмотров 4013 месяца назад
How to perform multiple sequence alignment without any software? | Mult Alin
A bioinformatics guide to Metabolomic Data analysis interpretation
Просмотров 1,6 тыс.3 месяца назад
A bioinformatics guide to Metabolomic Data analysis interpretation
How to a scale bar to microscopic images by Image J software
Просмотров 2654 месяца назад
How to a scale bar to microscopic images by Image J software
How to add a scale bar to camera or scanner images | Image J software
Просмотров 6234 месяца назад
How to add a scale bar to camera or scanner images | Image J software
How to draw a violin plot with statistics | T-test, Wilcoxon test, ANOVA, Kruskal-Wallis
Просмотров 2744 месяца назад
How to draw a violin plot with statistics | T-test, Wilcoxon test, ANOVA, Kruskal-Wallis
How to create a box plot with jitters?
Просмотров 2864 месяца назад
How to create a box plot with jitters?
How to interpret figures of Yeast Two-Hybrid for Protein Interactions
Просмотров 3254 месяца назад
How to interpret figures of Yeast Two-Hybrid for Protein Interactions
How to design gRNA for CRISPR genome editing? Hands-on training
Просмотров 1,1 тыс.5 месяцев назад
How to design gRNA for CRISPR genome editing? Hands-on training
editGPT: forget chatGPT: Best AI tool for research article
Просмотров 8165 месяцев назад
editGPT: forget chatGPT: Best AI tool for research article
what's that the table of evidence mean??
nature has created amazing orgnelles, nice video
❤️
how to get go id i only have ensp id
use go shiny tool then
@@asifmolbio thank you
@@asifmolbio but i want to get figures from chiplot
Sir i have few doubts can i email you
Sure write me asifalikalas@foxmail.com
@@asifmolbio thank you sir
Which genes do we use here? is it only for the disease we are targeting or the common targets from Jvenny?
Its for common
Thank you for your response. I will conduct experiments in mice, so for the selection of genes do I select the H.sapien option or the mouse genes option?
Thank you.great video
Glad you like it
Can we do gene ontology of a single gene ? If yes then plz guide ... Bcz in result error is coming
One gene is not possible
That one gene belongs to which specie?
@@asifmolbio Oreochromis niloticus
@@asifmolbio I want this analysis of a single gene .....
@@asifmolbio this gene is present in multiple spp
Sir from where to download the excel file data?
Example file is attached on the website
in circular tree ... u have added excel sheet in layer section... what type of data excel sheet contain? what we have to add because my color section is not coming
kindly help me?
Its just metadata like groups of species for example from rice can be A and from arabidopsis can be B
My tbtool isnt working, saying debug log, tell me how to fix this problem? I have unistalled it, while tried to download it again , the downloaded file was empty..
@@moulinahar9735 you can download from orginal website
Why its not taking my fasta format
Make sure there is no error in the file
Thank you so much for the detailed explanation in this video. It really helped.
Glad if it helped
The ability for some humans to live forever...
Not possible
Thank you for explaining in straightforward way. Keep going
Thanks
shut up, falsinformation
Thank you for a wonderful explanation, please is the control our reference gene while treatment our target gene. Thank You
@@okoseimiemaibifubara2080 thanks, no control is a separate treatment.
In my analysis, I used the reference gene and my genes of target is GH and IGF-1 with eight diets. How do I arrange it, please
@@okoseimiemaibifubara2080 follow like i have arranged and shown in example. In your case each Treatment (8 in your case ) will be compared with control separately.
Please where is your reference gene in the arrangement, Thank you
@@okoseimiemaibifubara2080 this data is from transcriptome let me give you a link of qPCR data.
wrong
whats wrong
Really ？
Yes 400 really
I have issue with mobile phone format code to vertified? Could you help me? Thanks
Please email to administrator. Their email is mentioned is help
Hello sir, How can I contact you? actually I need guidance regarding my analysis. Please let me know any platform to contact you .
asifalikalas@foxmail.com
@@asifmolbio Thank you sir
❤
It does not work with me i entered the data correctly? Please help
How to avoid common errors/problems in SR plot data analysis tool? ruclips.net/video/TB3C8g2PFDo/видео.html
Would this harm the computer in long run?
It might slow down a little overall no problem
Sir, can you please help me know how to find out a standard protocol with details for studying oxidative stress assays in zebrafish embryos?
Sorry i am not into animal science ask any relevant expert
Why we need Rice bromodomain sequence structure here?
I was just helping to reproduce results
@@asifmolbio So can we do it by any of gene sequences?
Great
Thanks
Simple but very helpful. I always refer your videos to my students. Thank you very much!
Thanks for recommending. Glad to know this. Stay in touch
Excellent lecture. Please keep posting on Linux
Sure thanks for interest
good work very helpful i need your contact number
Thanks. My email is mentioned in about section on my RUclips channel
Great teaching. Thank you Dr.Asif
Glad you like it
Sir please create a series of lectures on biological data analysis using Linux/Ubuntu
As soon as possible
If we take and edit pictures from ms designer to use it in our review paper, will it come under copyright or plagiarism?? If yess then is the site that you told in this video okay to use these graphics for review paper?
Should not exactly same to same
If idea is same you can cite like adopted from for example Ali et al.
@@asifmolbio okk thanks sir 🙌
I'm having trouble designing a guide RNA (gRNA) for a gene that's small, around 2300 bp, with 3 exons and 2 introns. The first two exons overlap in the 5' UTR region, and the third exon is partially in the 3' UTR. While using CHOPCHOP and CRISPR-P software for gRNA design, I'm not getting good results. The top-ranked gRNA has off-targets in exons of other loci on different chromosomes. My question is, how safe are off-targets, and if the mismatches or off-targets are in intergenic regions, should we consider using that gRNA? I'm quite confused about this. Please if it is possible for you kindly help
These are generally less concerning since these regions don't typically code for proteins or play direct roles in gene regulation. However, off-targets near regulatory elements or non-coding RNAs could still pose a risk
Kindly make a video on how to make map to show multi-location.
Sure, thanks for suggesting a potential topic
Could you tell me if it willl work with lipids?
If proteins IDs will work
Excellent website, thanks
Glad you like it
How to register on majorbio with an indian phone number ?
Its not possible to register by yourself but emailing to company will help you get started
good work🎉
Glad you like it
Hello Sir, I needed to download this data using wget in Linux. Any suggestions?
i only know how to get from web
Hello Sir I'm not able to see download option. They are asking to buy plan
Accept cookies of this website
@@asifmolbio I did accept all the cookies. It is showing me I can download only first 30 rows.
@@asifmolbio Kindly help me. I'm struck with my thesis work. This would help me a lot.
@@CosTheta-u1m you can send me search query to my email i will help you tomorrow
@@asifmolbio Thanks a lot sir. My name is Sushumna. I have sent you the query. Thank you
Thank you for good explaination Dr
Glad you like it
Sir I am trying to download gene list for GBM, but it is just downloading 1st 10 genes only and not all. What can I do?
@@anishachaturvedi9577 select to view 50 or 100 from drop down list then start downloading
@@asifmolbioThank you so much for replying. Actually there are 4429 genes, can I not get it all downloaded at once in a single excel sheet? If yes then how?
@@anishachaturvedi9577 i think it should be downloadable all together
@@asifmolbio it's not happening, just the 1st 32 genes are getting down 🙁
Unregistered users can only view 10 results, whether or not they accept the cookie policy. In order to make the most of the platform, you must register for an account. The plans are as follows: Free Academic: includes only curated data sets (in other words, the most well-known and fundamental info) accessible through the website and API, R package & Cytoscape App. (Full academic guide here) Standard: includes curated & text-mined data (in other words, newer discoveries and novel information), accessible only through the website. Advanced: includes same data as Standard, accessible through the website and API, R package & Cytoscape App.
please upload how to do Cluster and PCA analysis through R
Sure thanks for suggesting good topic
Just what I needed. Thank you!😊😊😊
Glad i helped you
Thanks Dr. Asif...Very Good Video...
Glad you you liked
Excellent Dr. Asif...thanks for help...
Welcome
I will do this for MIC and BMD
Sure
Subperb
Glad you like it
Thank you for informative video ❤
Glad it is helping