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Leica Microsystems Tutorials
Германия
Добавлен 8 июн 2020
Welcome to Leica Microsystems Tutorials!
Explore the world of microscopes and scientific instruments and get detailed applications tutorials from our team of Advanced Workflow Specialists.
Main channel: ruclips.net/user/leicamicrosystems
Explore the world of microscopes and scientific instruments and get detailed applications tutorials from our team of Advanced Workflow Specialists.
Main channel: ruclips.net/user/leicamicrosystems
Automated cell counting with the Mateo FL Digital Fluorescence Microscope
Obtain a swift and accurate cell count with Mateo FL in just 5 seconds, using automated cell counting, instead of 15 minutes of manual counting.
Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/
#digitalfluorescencemicroscope #confluencyanalysis #MateoFL
▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬
Automated cell counting with the Mateo FL Digital Fluorescence Microscope
Obtain a swift and accurate cell count with just a few clicks on your Mateo FL and forget about tedious manual counting.
Place your sample on the stage.
Now select your desired channel. You can choose between “Phase Contrast” for the transmitted light option or one of ...
Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/
#digitalfluorescencemicroscope #confluencyanalysis #MateoFL
▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬
Automated cell counting with the Mateo FL Digital Fluorescence Microscope
Obtain a swift and accurate cell count with just a few clicks on your Mateo FL and forget about tedious manual counting.
Place your sample on the stage.
Now select your desired channel. You can choose between “Phase Contrast” for the transmitted light option or one of ...
Просмотров: 308
Видео
Measure transfection efficiency with the Mateo FL Digital Fluorescence Microscope
Просмотров 2246 месяцев назад
Reliably and accurately check cell transfection efficiency with Mateo FL, eliminating the need for subjective estimations. Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/ #digitalfluorescencemicroscope #transfectionefficiency #MateoFL ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬ Measure transfection efficiency with the Mateo FL Digital...
Confluency measurement with the Mateo FL Digital Fluorescence Microscope
Просмотров 2246 месяцев назад
Find out how Mateo FL helps you eliminate guesswork and increase confidence in your downstream experiments using AI-assisted confluency measurements. Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/ #digitalfluorescencemicroscope #confluencyanalysis #MateoFL ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬ Confluency measurement with the Ma...
Unboxing and getting started with the Mateo FL Digital Fluorescence Microscope
Просмотров 3686 месяцев назад
Watch the video to find out how to easily set up your Mateo FL digital fluorescence microscope. Choose Mateo FL for reliable, reproducible advanced cell checks: www.leica-microsystems.com/products/light-microscopes/p/mateo/ #digitalfluorescencemicroscope #MateoFL ▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬ Unboxing and getting started with the Mateo FL Digital Fluorescence Microscope Welcome to Leica Microsystems...
UC Enuity Ultramicrotome - Automatic block face trimming in 2D
Просмотров 48210 месяцев назад
Empower your team through automation! Trimming a blockface is a time-intensive task and takes a certain level of training. UC Enuity takes over this task and handles the trimming. Simply define the boundaries of your sample block and walk away. 00:00 - Unveiling the UC Enuity Ultramicrotome 00:25 - Loading sample and knife 01:04 - Automated sample and knife movement 01:27 - Trimming in process ...
UC Enuity Ultramicrotome - Trim your target with the help of fluorescence
Просмотров 34310 месяцев назад
Trimming a block face is a time-intensive task and takes a certain level of training. In addition, sometimes the sample is buried inside of the resin and the target not visible increasing the risk to lose the sample during trimming. Using fluorescence imaging allows to visualize a fluorescent sample inside the resin. UC Enuity makes use of the fluorescence and enables a precise automatic trimmi...
UC Enuity Ultramicrotome - Auto Alignment of block face and knife
Просмотров 43610 месяцев назад
Empower your team through automation! Have you ever struggled with aligning block face and knife for getting good sections on the ultramicrotome? UC Enuity takes over this task and automatically aligns block face and knife for highest sectioning quality. 00:00 - Unveiling the UC Enuity Ultramicrotome 00:24 - Loading knife and sample 00:47 - Start of the automatic alignment 01:14 - Start of sect...
UC Enuity Ultramicrotome - Trim your target using 3D-µCT data
Просмотров 42010 месяцев назад
Rely on UC Enuity to reach the burried target! Trimming a block face is a time-intensive task and takes a certain level of training. In addition, sometimes the sample is burried inside of the resin and the target not visible increasing the risk to loose the sample during trimming. Using a µCT allows to visualize the sample inside the resin. UC Enuity makes use of the µCT-data and enables a prec...
How to activate the confluency module for the Mateo TL microscope
Просмотров 224Год назад
You can perform consistent confluency measurements with Mateo TL, our digital transmitted light microscope. It increases your confidence in the success of downstream experiments. The Confluency module can be used to estimate cell confluency, the percentage of the surface of a culture vessel that is covered by adherent cells. To activate the confluency module please follow the steps within this ...
How to use the Proveo 8 ophthalmic microscope?
Просмотров 1,9 тыс.Год назад
Tutorial video on how to use the Proveo 8 ophthalmic surgical microscope. Introduction to microscope setup and basic controls. 00:00 - How to use the Proveo 8 ophthalmic microscope? 00:08 - Microscope Basic Setup 00:12 - How to activate the base brake 00:21 - How to set up your user program 00:50 - How to record videos 01:34 - How to position the integrated monitor 01:43 - How to position the o...
Documentation & Sharing with Enersight
Просмотров 863Год назад
This video describes where your files are saved, how to connect your device to the network, how to share images by all operations modes (Enersight OSD, Desktop, Mobile) and save them on network folders. Learn more: fcld.ly/tl0zlet 00:00 - Documentation & Sharing 00:12 - Save files locally by Enersight Desktop, Enersight On-Screen Display and Enersight Mobile 01:07 - Save files in the network fo...
Measurement, annotations, and calibration with Enersight
Просмотров 1,5 тыс.Год назад
This video describes how to: - calibrate your system - perform measurements - annotate a live image - acquire an image with measurements - save measurement or annotations as an overlay - edit previously created measurements and annotations Learn more: fcld.ly/tl0zlet 00:00 - Calibration 01:56 - Measurement 02:58 - Set up a tolerance 03:27 - Annotation 03:59 - Change the properties of drawn elem...
Comparison & Overlays with Enersight
Просмотров 554Год назад
This video describes how to: - compare a live image with a reference image with a single click - acquire a single image showing a comparison view of your sample to a reference image - apply overlays to the live image - create and edit custom overlays - upload and use custom overlays - configure a footswitch or middle mouse control to toggle between custom overlays or recall the last used overla...
Image acquisition & Video recording with Enersight
Просмотров 628Год назад
This video describes how to acquire image and record videos with Enersight. This video is also about the Gallery function and how to change the format and resolution of an image. Learn more: fcld.ly/tl0zlet 00:00 - Image acquisition 00:41 - Video recording 01:21 - Gallery 03:07 - Resolution and format change 03:38 - Stamp and scale bar #enersight #inspection #microscope #visualinspection #quali...
Live streaming & Camera control with Enersight
Просмотров 801Год назад
This video describes how to create a live stream movie of your sample and how to control the camera via Enersight software platform. Learn more: fcld.ly/tl0zlet #enersight #inspection #microscope #visualinspection #qualitycontrol ▬ More Videos ▬▬▬▬▬▬▬▬▬▬▬▬ Subscribe @LeicaMicrosystems @LeicaMicrosystemsTutorials ▬ Follow us ▬▬▬▬▬▬▬▬▬▬▬▬ Discover us on LinkedIn: www.linkedin.com/company/leica-mi...
First steps with Enersight software platform
Просмотров 1,7 тыс.Год назад
First steps with Enersight software platform
Laser Microdissection for Spatial Proteomics
Просмотров 2,6 тыс.Год назад
Laser Microdissection for Spatial Proteomics
Consistent operation that saves you time
Просмотров 569Год назад
Consistent operation that saves you time
Directly compare to references with a single click
Просмотров 579Год назад
Directly compare to references with a single click
Identify defects more clearly during visual inspection
Просмотров 784Год назад
Identify defects more clearly during visual inspection
Measure directly during visual inspection
Просмотров 1,4 тыс.Год назад
Measure directly during visual inspection
How to set up multiple fluorescent dyes for sequential imaging
Просмотров 1,1 тыс.Год назад
How to set up multiple fluorescent dyes for sequential imaging
How to start and use the Software Guide and where to find the Online Help
Просмотров 932Год назад
How to start and use the Software Guide and where to find the Online Help
How to set up Mica for different sample vessels and image acquisition
Просмотров 1,2 тыс.Год назад
How to set up Mica for different sample vessels and image acquisition
How to extract quantitative data from your image using the AI pixel classifier
Просмотров 1,6 тыс.Год назад
How to extract quantitative data from your image using the AI pixel classifier
How to optimize your acquisition using Mica’s intelligent imaging
Просмотров 1,2 тыс.Год назад
How to optimize your acquisition using Mica’s intelligent imaging
Aivia Web guided tour - Your journey to AI-powered image analysis
Просмотров 844Год назад
Aivia Web guided tour - Your journey to AI-powered image analysis
How to repeat image acquisition conditions with the Mateo TL microscope
Просмотров 2942 года назад
How to repeat image acquisition conditions with the Mateo TL microscope
Confluency measurement with the Mateo TL microscope
Просмотров 8812 года назад
Confluency measurement with the Mateo TL microscope
How to export images to a USB drive with the Mateo TL microscope
Просмотров 6972 года назад
How to export images to a USB drive with the Mateo TL microscope
How to save those 3d images? or they are just to view?
Hi there! On the bottom of the menu on the left, under "Movie Editor" it should allow you to save in different formats.
Thanks for the Explanation, Informative
how to connect it to a pc or something that send email and alert
Dear zz6761, thank you for contacting us! The easiest way to get in touch with our support team would be by filling out this form: fcld.ly/contactsupport Please let us know if you need any assistance. Best regards, your Leica Microsystems Social Media Team
You can use Sensaphone to set up an alert system. It connects to the output port at the back of the EM TP and sends an alert when the processor gives an error. You can set it up to send texts, voice and emal messages to a list of people. It will not tell you what the error is, so we also set up a webcam so if we are out of the lab we can see the screen and see what the error is and what step it is on.
But how do you create and retain calibration data after you change the magnification of the objective lens?
@burkekorol2910 Hi, thanks for your question! In short, the answer is: calibration can be done and saved under Measurements Tab -> Calibration. We can provide a more detailed answer, depending on your specific device (Enersight compatible stand or only Enersight compatible camera). Please contact us through customer service with your device details and we can provide better help that way! 👉fcld.ly/contact-gen
Ok, but like what is an easy way to re-save those individual image files as tifs? I don't know why Leica forces our images to be compiled into a .lif in the first place; it just causes unnecessary work
Hello, .lif is the default file format within LAS X, but you can easily export to tif. This video tutorial goes over how to do that: ruclips.net/video/wvQ1IC7euvs/видео.html If you have additional questions, please contact us: www.leica-microsystems.com/contact/contact-us-online/
To best support you, and reach an application specialist, please contact us here: www.leica-microsystems.com/contact/contact-us-online/ Kind Regards, Leica Microsystems Social Media Team
do you have a microscopic slide collector device?
Hi there, thank you for your interest! Please reach out to our product team for more information here: fcld.ly/sdk23ds 😊
Cool
Hello, Thanks for your helpful video! I have a question regarding time-lapse imaging using Leica! I am going to do time-lapse imaging (follow for 3 days) but I have no idea about the flow rate of water (from water pump for the objective 63X). Unfortunately, I could not find any sources to solve my problem. I wonder if you could please tell me the appropriate flow rate. Thanks!
Hi Abolfazl! Please reach out to the support team by filling out this Contact Us form: www.leica-microsystems.com/contact/contact-us-online/
veramente interessante , un bel prodotto
100% special and temporal correlation? This statement can’t be supported from philosophical, quantum mechanical and classical light theory points of view.
Hi there.. focusing could be done through microscope only or it could be done in these settings area while projecting and capturing the image?
Hi, focusing can done through both the eyepieces as well as on the monitor through the software. We recommend that, for setting up Z-stacks, a live preview in LAS X is used, in case there is any discrepancy between the focus through the eyepieces versus the camera.
Super useful information! Very cool!
Hello, I was wondering what can be done when the following message appears as you press the RFC button: Switch to z-wide and deactivate sequencer board to apply relative focus correction or activate the advance sequencer board. This message just appear and the window you have in the video is not popping up. Thanks!
Hi Raquel - go to the Project Settings Window and uncheck the Advanced Sequencer option. Then you should be able to use RFC.
How to export the huge image (navigator image)? I already reduced the tilescan size to 1% but the software still didn't allow me to export the image.
Hi Zichun - please contact your local Leica specialist or call 866.830.0735 x3 for help with exporting large files.