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- Видео 52
- Просмотров 53 122
Natalia Tretyakova
Добавлен 26 мар 2016
Видео
Tretyakova Lab • Ryan Friess Interview
Просмотров 2021 день назад
Tretyakova Lab • Ryan Friess Interview
Tretyakova Lab • Qui Zhang Interview
Просмотров 8610 месяцев назад
Tretyakova Lab • Qui Zhang Interview
Tretyakova Lab • Allysha O'Donnell Interview
Просмотров 6710 месяцев назад
Tretyakova Lab • Allysha O'Donnell Interview
Tretyakova Lab • Joe McPherson Interview
Просмотров 8011 месяцев назад
Tretyakova Lab • Joe McPherson Interview
Tretyakova Lab • Eric Moran Interview
Просмотров 5411 месяцев назад
Tretyakova Lab • Eric Moran Interview
Tretyakova Lab • Methylated DNA Immunopreciptation
Просмотров 183Год назад
Tretyakova Lab • Methylated DNA Immunopreciptation
Tretyakova Lab • Primer Extension Assay
Просмотров 385Год назад
Tretyakova Lab • Primer Extension Assay
Tretyakova Lab • Proteomics Isobaric Labeling Protocol
Просмотров 4822 года назад
Tretyakova Lab • Proteomics Isobaric Labeling Protocol
Tretyakova Lab • Biotage Isolera One User Guide
Просмотров 7 тыс.2 года назад
Tretyakova Lab • Biotage Isolera One User Guide
Tretyakova Lab • Streaking Colonies on Agarose Gel
Просмотров 1382 года назад
Tretyakova Lab • Streaking Colonies on Agarose Gel
Tretyakova Lab • HPLC Mass-Spectrometry Sample Prep
Просмотров 5 тыс.2 года назад
Tretyakova Lab • HPLC Mass-Spectrometry Sample Prep
Tretyakova Lab • Organic Synthesis Protocol
Просмотров 24 тыс.2 года назад
Tretyakova Lab • Organic Synthesis Protocol
Tretyakova Lab • Silica Column Prep
Просмотров 3,5 тыс.2 года назад
Tretyakova Lab • Silica Column Prep
ACS TOXI Dr Amanda Bryant-Friedrich Interview
Просмотров 1402 года назад
ACS TOXI Dr Amanda Bryant-Friedrich Interview
good research, ill keep this in mind when applying to grad schools
It is very interesting to see that how new scientist are getting created with proper training😇
Allah bless you
We proud on you brother 🥰
Does this represent Darwin's "warm little pond?"
The oligo synthesis was in fact one of my wonder that made possible molecular biology enjoyable.
i exptress my interest to say that am happy to listen from you my bruh, continue in that way its better
Excellent. Thank you!
thank you!
Thank you. Excellent explanation and demonstration
A general question regarding contamination: Is it a routine to spray alcohol absolutely on every object that is inserted in the laminar flow cabinet. But how about the flasks that are transported to the microscope for checking stem cell detaching ? I did not see in any video on RUclips such an operation of spraying the flask before reintroducing it in the laminar flow cabinet !
How to identify the ports when 2 portsor compartments r present for columns
Nice
imagine being a doctor and still thinking its called mass spectroscopy
А чего так всё на английском? Оборудование вроде норм, +/-.
Thank you .please how many silica gel can we use for sample?
After doing rota we do vaccum also. So that it get dried completely
Good idea!
Slowly this is funny part, and colone for amount this SiO2 is too much funny.😀😀😀😀
This video is very useful. Thank you so much 😊
Cool!!!
The most beautiful
Hi, Thank you for your video, really helpful for me. Do we need to install the spacer again if we want to do wet loading? you removed the spacer for dry loading, but how about wet loading?
This is an excellent demosntration of PEA! Thank you.
Thank you very much
I'm research scientist working in discovery chemistry medicine and medicinal chemistry, I have good experience handling multiple different reactions...... I am looking for PHD is there any chance in your group to work with scholarship
Thanks for showing this tutorial!
I love the biotoage instruments. We have a bit newer one which is even more helpful!
クルードした後は、フラッシュですか?オープンですか?
this is a crappy way of doing it and why DCM? no explanation at all!
Free flowing slurry are formed in DCM...
展開溶媒をDCMと省略しているのでは?
You are doing great work mam, Keep it up
Thank you for the video. Please teach me how long it takes to purge the flask with gas using the balloon? I need to use argon in our lab too.
Желаю успехов!
When doing the TLC how do you know the one on the right is the product? The RF is almost the same for the one on the left, but because left is way more concentrated, it had caused streaking.
Thanks for the comments, Mairis! Well, for this particular reaction the RF is almost the same. We have characterized the spot (compound) thoroughly using NMR and Mass spectroscopy techniques.
Dilute your sample. Switch solvents. 1-Chlorobutane is a great alternative to hexane. Similar to 15-25% EtOAc:Hex, but it has a tendency to separate things EtOAc:Hex, will not. Also, it flips the spots, sometimes. This makes it easier, if one wants the now top spot, that was bottom in EtOAc:Hex. Just cause its a new spot, doesn't mean it is the desired product.
In this type of case another eluent system including a protic polar phase like 99:1 - 95:5 DCM/MeOH is useful to remove the streaking by favoring H-bonding of the starting material with the eluent. If you have two spots at the same height, you can also reveal with various stains. Some might be selective of your starting material and allow for proper monitoring. A good start for stains screening can be p-anisaldehyde, CAN, nihnydrin, I2 and KMnO4. If none of them are selective of your starting material, you can still do a MS without forgetting to record a spectra of the starting material as a reference. PS: TLC are better run with a co-spot. Thanks to Tretyakova lab for the content.
Concentration is toooo high on left, reduce concentration, try different combinations of solvent
Thank you for the video, it is very useful and contains many useful tips.
Thank you ❤️