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This muntjak deer was filmed in our garden today. Hope you enjoy, we did!

Presentation to the Public Meeting organised by Oxford Flood and Environmental Group held a West Oxford Community Centre on 19th November 2021. Created by Rod Chalk of Hinksey and Osney Environmental Group.

Do you really want to understand mass spec? Things are not quite as they seem..............

Due to its speed and simplicity, MALDI lends itself well to a variety of routine and high throughput applications. As a niche technique it has important applications in clinical diagnostics and tissue imaging.

HPLC is an essential practical skill for mass spectrometry and across the physical sciences. In the penultimate seminar in the series, Rod Chalk discusses system components, mobile phases, gradients, columns, junctions, nano flow and basic trouble shooting.

Precision matters in small molecule mass spectrometry. Rod Chalk explains why a compound has four different values for its mass and discusses how to calibrate instruments, perform LC-MS on any type of compound and interpret spectra. Discover how monoisotopic mass, mass defect, isotope matching and the nitrogen rule are used to determine the composition of an unknown compound.

An introduction to metabolomics: Investigating cellular function and determining novel drug targets for disease. James McCullagh is Professor of Biological Chemistry and Director of the Mass Spectrometry Research Facility in the Department of Chemistry at the University of Oxford. He utilises a range of bioanalytical techniques, in particular mass spectrometry, to perform metabolomics experimen...

Any ionisable species can be quantified by mass spectrometry with high sensitivity and absolute specificity, making it fundamental to DMPK, enzyme kinetics and proteomics. In the 11th seminar of the series Rod Chalk discusses factors affecting ion yield, and the different approaches to relative and absolute MRM quantitation using real examples from Oxford's Centre for Medicine Discovery.

Will Greenland has been collaborating with Oxford’s Centre for Medicines Discovery using mass spectrometry to characterise complex glycosylation present on the SARS-Cov2 Spike Protein. He will explore why protein glycosylation is important, and using Spike Protein as an example, why glycoprotein analysis is difficult.

Dr Holger Kramer, Head of Biological Mass Spectrometry & Proteomics, MRC London Institute of Medical Sciences will discuss strategies for quantitative bottom-up proteomics analysis of complex biological samples. Different approaches to data acquisition, including Data-Dependent Acquisition (DDA), Data-Independent Acquisition (DIA) and targeted proteomics analysis will be highlighted. With a foc...

Phosphorylation plays a central role in molecular signalling with an estimated 30-65% of human proteins phosphorylated. Although global approaches to phosphoryation have severe limitations, with the aid of real examples, Rod Chalk explains how these limitations can be overcome.

Membrane proteins are amenable to all MS analysis methods. In the eighth seminar of the series, Rod Chalk discusses detergent removal off-line, by in-line chromatographic separation and post-ionisation, as well as detergent-free analyses. Identity, structure and functional data gained in this way are powerful tools to accelerate membrane protein research.

Limited proteolysis, chemical derivitisation, cross-linking & hydrogen-deuterium exchange make all aspects of structural biology amenable to MS analysis. In the seventh seminar of the series, Rod Chalk shows how these different experimental approaches can be successfuly applied to answer specific structural questions.

Native MS allows solution-phase molecular interactions and time-resolved multiple biophysical parameters to determined in a single experiment. In the sixth seminar in the series, Rod Chalk describes practical aspects of the technique, and shows how a proper understanding of the electrospray ionisation mechanism makes native MS the most powerful benchtop analytical technique available in protein...

5 Principles of Intact Mass Analysis

3 Interpreting Mascot data (practical)

2 Protein Analysis using Tandem Mass Spectrometry

1 Fundamentals of Mass Spectrometry

Theory and Practice of Native Mass Spectrometry 2 of 2

Theory and Practice of Native Mass Spectrometry 1 of 2