Study Tech
Study Tech
  • Видео 35
  • Просмотров 14 143
BWA | Install | Build index | Alignment #bioinformatics #biology #tutorial
-------- BWA tool link
github.com/lh3/bwa
--------- BWA Script installation
alex@alex:~/Downloads/bwa-master$ make
--------- BWA Script for build index
alex@alex:~/Downloads/bwa-master$ ./bwa index ../Saccharomyces_cerevisiae.R64-1-1.dna.toplevel.fa
********* Run Alignment [*There is an angled bracket in this script which is missing, see the video ]
alex@alex:~/Downloads/bwa-master$ ./bwa mem ../Saccharomyces_cerevisiae.R64-1-1.dna.toplevel.fa ../Read1_forward.fastq ../Read1_reverse.fastq | gzip -3 * aligned.sam.gz
* RUclips does not allow writing angled brackets in the description box. So it is missing.
leave a comment to report an error on this video
----------- Quick note
01:23 Installation
03:...
Просмотров: 84

Видео

STAR | sequence alignment | mapping #bioinformatics #biology #tutorial
Просмотров 10821 день назад
STAR tool link bioinformaticshome.com/tools/rna-seq/descriptions/STAR.html#gsc.tab=0 STAR Script installation cd STAR/source make STAR STAR Script for build index ./STAR runMode genomeGenerate genomeDir /home/alex/Downloads/genomeDir genomeFastaFiles /home/alex/Downloads/Saccharomyces_cerevisiae.R64-1-1.dna.toplevel.fa sjdbGTFfile /home/alex/Downloads/Saccharomyces_cerevisiae.R64-1-1.113.gtf sj...
STAR | Install | Build index | #bioinformatics #biology #tutorial #ngsitsme
Просмотров 112Месяц назад
STAR tool link bioinformaticshome.com/tools/rna-seq/descriptions/STAR.html#gsc.tab=0 STAR Script installation cd STAR/source make STAR STAR Script for build index ./STAR runMode genomeGenerate genomeDir /home/alex/Downloads/genomeDir genomeFastaFiles /home/alex/Downloads/Saccharomyces_cerevisiae.R64-1-1.dna.toplevel.fa sjdbGTFfile /home/alex/Downloads/Saccharomyces_cerevisiae.R64-1-1.113.gtf sj...
Bowtie2 | Install | Build Index | Alignment | Paired end sequence #bioinformatics #tutorial
Просмотров 70Месяц назад
Bowtie2 Source code github.com/BenLangmead/bowtie2 Bowtie2 Manual bowtie-bio.sourceforge.net/bowtie2/manual.shtml#building-from-source Bowtie2 Script installation make static-libs && make STATIC_BUILD=1 Check installation ./bowtie2-build -h ./bowtie2 -h Build index ./bowtie2-build /home/alex/Downloads/Saccharomyces_cerevisiae.R64-1-1.dna.toplevel.fa /home/alex/Downloads/genomeDir/index Alignmen...
GFF/GFF3 to GTF by gffread| Install | Run | #bioinformatics #biology #tutorial
Просмотров 71Месяц назад
gffread tool link github.com/gpertea/gffread gffread Script installation cd gffread make release gffread Script run ./gffread TAIR10_GFF3_genes.gff -T -o Tair10.gtf ./gffread Arabidopsis_thaliana.gff3 -T -o from_gff3.gtf Timeline 02:20 Installation 03:34 Convert GFF to GTF 06:25 Convert GFF3 to GTF System configuration Processor- Intel i5 4th gen, 1.7 GHz, 4 CPU(or thread) RAM-8GB Boot and work...
Fastp | Install | Run | Paired end #bioinformatics #biology #tutorial #NGS
Просмотров 105Месяц назад
Fastp tool link github.com/OpenGene/fastp Fastp Script installation wget opengene.org/fastp/fastp chmod a x ./fastp Fastp Script run ./fastp -i Read1_forward.fastq -I Read1_reverse.fastq -o trimmed_for.fq -O trimmed_rev.fq Reference paper academic.oup.com/bioinformatics/article/34/17/i884/5093234 System configuration Processor- Intel i5 4th gen, 1.7 GHz, 4 CPU(or thread) RAM-8GB Boot and workin...
TrimGalore | Install | Run | Paired end #bioinformatics #biology
Просмотров 252Месяц назад
TrimGalore tool link www.bioinformatics.babraham.ac.uk/projects/trim_galore/ github.com/FelixKrueger/TrimGalore/tree/master TrimGalore Script ./trim_galore -q 20 paired Read1_forward.fastq Read1_Reverse.fastq System configuration Processor- Intel i5 4th gen, 1.7 GHz, 4 CPU(or thread) RAM-8GB Boot and working drive- 256GB SSD OS- Linux Mint 21.1 Cinnamon Guys, if you need any tutorial or help re...
Cutadapt tutorial 🛠️Install 💻 Run 🎓 Paired end #bioinformatics #biology
Просмотров 1472 месяца назад
Cutadapt tool link cutadapt.readthedocs.io/en/stable/ cutadapt.readthedocs.io/en/stable/installation.html Terminal Script cutadapt -o out1.fastq -p out2.fastq SRR30685383_1.fastq SRR30685383_2.fastq System configuration Processor- Intel i5 4th gen, 1.7 GHz, 4 CPU(or thread) RAM-8GB Boot and working drive- 256GB SSD OS- Linux Mint 21.1 Cinnamon Guys, if you need any tutorial or help regarding Bi...
Trimmomatic tutorial 🛠️Install 💻 Run 🎓Single End 🎓 Paired end #bioinformatics #biology
Просмотров 872 месяца назад
Trimmomatic tool link www.usadellab.org/cms/?page=trimmomatic System configuration Processor- Intel i5 4th gen, 1.7 GHz, 4 CPU(or thread) RAM-8GB Boot and working drive- 256GB SSD OS- Linux Mint 21.1 Cinnamon Guys, if you need any tutorial or help regarding Bioinformatics work, we will try to help you by Video or Chat. Fill free to ask. If you are very new to Bioinformatics and have some very b...
SRA to fastq file conversion || SRA toolkit || NCBI
Просмотров 5722 года назад
Guys, if you need any tutorial or help regarding Bioinformatics work, we will try to help you by Video or Chat. Fill free to ask. If you are very new to Bioinformatics and have some very basic and silly questions. You are most welcome. Don't hesitate to ask us. You can write in our Google form forms.gle/UjGFtBu8Ln36J41Y8
SRA tool kit || installation to SRA file downloaded completely || Beginner
Просмотров 3912 года назад
Download NGS data How to download sequencing data Guys, if you need any tutorial or help regarding Bioinformatics work, we will try to help you by Video or Chat. Fill free to ask. If you are very new to Bioinformatics and have some very basic and silly questions. You are most welcome. Don't hesitate to ask us. You can write in our Google form forms.gle/UjGFtBu8Ln36J41Y8
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Просмотров 143 года назад
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Download Fastqc tool
Просмотров 1,5 тыс.4 года назад
Guys, if you need any tutorial or help regarding Bioinformatics work, we will try to help you by Video or Chat. Fill free to ask. If you are very new to Bioinformatics and have some very basic and silly questions. You are most welcome. Don't hesitate to ask us. You can write in our Google form forms.gle/UjGFtBu8Ln36J41Y8
Bowtie tutorial || beginner ||
Просмотров 5 тыс.4 года назад
Guys, if you need any tutorial or help regarding Bioinformatics work, we will try to help you by Video or Chat. Fill free to ask. If you are very new to Bioinformatics and have some very basic and silly questions. You are most welcome. Don't hesitate to ask us. You can write in our Google form forms.gle/UjGFtBu8Ln36J41Y8
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