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Yes!!! The greatest day of my life!!!

Frfr!!! Keep up the quality posts!!!

To check for the possibility that the solvent (deionized water typically) that you used to make calibration solutions (standard solutions) contains any of the ions that you are measuring.

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The suppressor has three chambers. At any given time, eluent flows through one, sulfuric acid through another, and deionized water through the third. The role of the chambers cycles periodically (every ~10 minutes). The eluent one becomes the acid one, the acid one becomes the water one and the water one is brought into use with the eluent. The one flowing eluent is doing the suppression, the one flowing acid is regenerating, and the one flowing water is rinsing away excess sulfuric acid (to get it ready for it's next role suppressing the eluent conductivity).

@@robinstoodley9431 Wow... Great explanation, your text is as good as animation. Thanks Robin!!

Absolute calibration of the conductivity detector is done at the factory and normally does not ever need re-doing. Relative calibration done daily by measuring a blank sample; perhaps deionized water.

@@robinstoodley9431 sir if Conductivity will be less than that what we do to rectify the instrument

The contribution from electroosomosis is much greater than the contribution from electrophoresis (mainly because of the excess positive charge on the diffusive layer that drags the entire buffer solution (positive, negative and neutral ions) towards the cathode. In other words, this electroosmosis is originated mainly because of the nature of the negative charges on the inner wall of the capillary, which then acts accordingly to the applied voltage. For example, if no high voltage is applied across the capillary, only a double layer will form which is still rich with excess positive charge in the diffusive part to compensate the excess negative charge.

You can't have the electrophoretic mobility without also having the electroosmotic flow. As soon as you apply the electric field to the capillary, you get both.

Especially the animated part, thank you

same question!

@@bim1537 Sketched what I wanted the animations to show, then hired a graphic artist to make it all look beautiful. Had to spend a while correcting the artist's work to ensure scientific correctness!

U must be scientist now mam?