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Refeyn
Великобритания
Добавлен 27 мар 2020
We are pioneers of mass photometry, a revolutionary new technology for analysing biomolecules.
Mass photometry is a revolutionary new way to analyze molecules. It enables the accurate mass measurement of single molecules in solution, in their native state and without the need for labels. This approach opens up new possibilities for bioanalytics and research into the functions of biomolecules.
Find our latest video resources on RUclips, including webinars, tutorials, and much more!
Mass photometry is a revolutionary new way to analyze molecules. It enables the accurate mass measurement of single molecules in solution, in their native state and without the need for labels. This approach opens up new possibilities for bioanalytics and research into the functions of biomolecules.
Find our latest video resources on RUclips, including webinars, tutorials, and much more!
Using mass photometry to power up the development of AAV-based therapeutics
Using mass photometry to power up the development of AAV-based therapeutics
Просмотров: 253
Видео
Mass photometry to study the oligomerization of the ubiquitin ligase HACE1
Просмотров 2713 месяца назад
Ubiquitin ligases (E3) are therapeutically relevant enzymes that orchestrate many key cellular pathways. This makes it important to understand the mechanisms that regulate their activity and substrate specificity. In this webinar, Sonja Lorenz - Group Leader at the Max Planck Institute for Multidisciplinary Sciences - explains how she used mass photometry for the study of the HACE1 ubiquitin li...
Characterizing oligomerization dynamics of an E3 ligase using mass photometry
Просмотров 1123 месяца назад
In this webinar, Refeyn travel grant winner Xiaojing Huang explains how her research harnessed the power of mass photometry to analyze the oligomeric state of the DCAF15-DDA1-DDB1 E3 ligase at low nanomolar concentrations. This study unveiled, for the first time, the formation of dimers and trimers with quantified dissociation constants. The oligomeric remodeling effect emphasized the transitio...
Measuring viral vector purity, stability and physical titer in minutes - with just one tool
Просмотров 1623 месяца назад
In this webinar, Refeyn’s Laura Pala presents the KaritroMP macro mass photometer, an instrument that can quickly characterize samples of viral vectors like adenoviruses and lentiviruses. The webinar explains how macro mass photometry works and how it addresses common analytical challenges faced in viral vector production. It also shows the application of macro mass photometry to the characteri...
Using mass photometry for AAV analytics
Просмотров 3,8 тыс.3 месяца назад
Using mass photometry for AAV analytics
Accelerating protein characterization with mass photometry
Просмотров 2384 месяца назад
In this webinar, Refeyn’s Tomás de Garay gives an overview of the principles behind mass photometry, its main applications and the types of molecules it can analyze. He also talks about how mass photometry compares to other analytical techniques such as cryo-EM, SEC, and mass spectrometry. Finally, he discusses technical questions and practical considerations with Scott Hanton, the editorial di...
Studying Lipoprotein Lipase oligomerization dynamics with cryoEM and mass photometry
Просмотров 1534 месяца назад
In this webinar, you will learn how Dr. Kathryn Gunn and her colleagues from University of North Carolina at Chapel Hill, solved the structure of an active lipoprotein lipase (LPL) dimer and determined its oligomerization behaviours using cutting edge bioanalytical methods. You will discover how mass photometry can complement cryoEM data - determining the dynamic oligomerization states of LPL i...
Rapid analytical approach for biologics characterization - mass photometry
Просмотров 1826 месяцев назад
In this webinar you will learn - via example data and case studies - how mass photometry supports the development and production of therapeutic biologics such as antibodies and AAVs. Mass photometry is a powerful bioanalytical tool that characterizes samples at the single-molecule level with low sample consumption, fast turnaround times and no need for labels. These strengths make mass photomet...
Therapeutic antibody stability - a rapid method for forced antibody degradation studies
Просмотров 1856 месяцев назад
Forced antibody degradation studies are essential for evaluating susceptibility to aggregation or fragmentation, critical in biotherapeutic development. In these studies, analytical tools play a pivotal role, ensuring precise characterization of samples exposed to different conditions. In this webinar, you can learn how mass photometry, a column-free technology, can quickly assess antibody samp...
Rapidly characterizing complex samples with mass photometry
Просмотров 2837 месяцев назад
In this webinar, Perla Vega, Technical Sales Specialist at Refeyn, introduces the principles and general applications of mass photometry, explaining how its fast, single-molecule characterization with low sample requirements can be helpful for a broad range of applications - from antibody aggregation to protein-DNA interactions. After this general introduction, Dr. Philip Kitchen from Aston Uni...
An essential PAT technology for downstream processing in AAV manufacturing
Просмотров 2117 месяцев назад
Amidst soaring demand for gene therapies using adeno-associated virus (AAV) vectors, robust and efficient analytical methods are pivotal for ensuring high AAV production quality. Get to know mass photometry - a game-changer in AAV downstream processing. Quick, precise, and serotype-agnostic, it consistently measures empty-full and partially filled AAVs with minimal sample requirements. In this ...
Join us at the Mass Photometry Summit 2024!
Просмотров 1517 месяцев назад
We are excited to announce the 2024 Mass Photometry Summit, taking place in Boston, US. This in-person event offers scientists in North America the opportunity to engage in scientific discussions on mass photometry, hear new product announcements, participate in demos, and more. Join us for the day and be immersed in knowledge of the latest advancements in mass photometry.
Tau protein aggregation & neurodegenerative disease; new insights from mass photometry
Просмотров 4509 месяцев назад
Tau aggregation is a critical process associated with neurodegenerative diseases like Alzheimer's and Parkinson's. Traditional analytical methods that are used to study aggregation often fall short in quantifying small, yet highly neurotoxic, tau protein oligomers. In this webinar, you will learn how Dr. Simanta Sarani Paul and his colleagues from the University of Alberta used mass photometry,...
Advances in cell and gene therapy: Characterizing large viral vectors using macro mass photometry
Просмотров 2729 месяцев назад
Accurate characterization of large viral vectors is a critical step in the development of viral vector-based gene therapies and vaccines. Refeyn is excited to introduce the KaritroMP, a novel analytical instrument specifically designed for characterizing large viral vectors in R&D environments. In this webinar, Refeyn’s Laura Pala will present case studies and benchmark results on how the Karit...
Characterizing protein oligomerization dynamics with automated mass photometry
Просмотров 3269 месяцев назад
Even though protein oligomerization is central to many cellular processes, its measurement can be challenging. Mass photometry is especially suited for assessing protein oligomerization, since it can quantify the relative abundance of a protein’s oligomeric states, including rare species. Automated mass photometry builds on these strengths, offering rapid, reproducible measurements of multiple ...
Mass photometry for biologics development (antibody and AAV analytics)
Просмотров 29410 месяцев назад
Mass photometry for biologics development (antibody and AAV analytics)
Rapid and serotype-agnostic analytics of viral vector samples with mass photometry
Просмотров 24010 месяцев назад
Rapid and serotype-agnostic analytics of viral vector samples with mass photometry
Streamlining AAV Characterization with Mass Photometry
Просмотров 26310 месяцев назад
Streamlining AAV Characterization with Mass Photometry
Harnessing mass photometry for AAV sample characterization in GMP-regulated environments
Просмотров 493Год назад
Harnessing mass photometry for AAV sample characterization in GMP-regulated environments
Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis
Просмотров 190Год назад
Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis
Monitoring aggregation levels of biosimilar mAbs using mass photometry and SEC
Просмотров 652Год назад
Monitoring aggregation levels of biosimilar mAbs using mass photometry and SEC
Assessing production variability in adeno-associated viruses by single-particle mass analysis
Просмотров 370Год назад
Assessing production variability in adeno-associated viruses by single-particle mass analysis
Studying protein polymerization with mass photometry.
Просмотров 502Год назад
Studying protein polymerization with mass photometry.
Demonstrating Analytical Comparability in Analytical Development
Просмотров 549Год назад
Demonstrating Analytical Comparability in Analytical Development
Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
Просмотров 289Год назад
Elucidating the composition of CRISPR-Cas12f1 complexes using mass photometry
Studying Form I Rubisco using ancestral sequence reconstruction and mass photometry
Просмотров 197Год назад
Studying Form I Rubisco using ancestral sequence reconstruction and mass photometry
Streamlining AAV characterization with automated mass photometry
Просмотров 553Год назад
Streamlining AAV characterization with automated mass photometry
[Webinar] La photométrie de masse: Caractériser vos molécules en 5 minutes!
Просмотров 342Год назад
[Webinar] La photométrie de masse: Caractériser vos molécules en 5 minutes!
How does Mass Photometry work? [with subtitles]
Просмотров 6 тыс.Год назад
How does Mass Photometry work? [with subtitles]
Can poly_D_lysine be used instead of poly_L_lysine? Should these slides be freshly made, or can they be stored for a long time?
Do you know why this technique uses green light?
Can you comment on the reproducibility of the data, since the sample volume is very low, it may be prone to sampling bias and that can be addressed with multiple sampling. Has this been shown to be needed or is sampling bias not an issue.
Promo'SM
Which proteins are used for this calibration? Thank you
Hi Luke, the protein used was beta amylase (56, 112 and 224 kDa).
@@refeyn thank you! do you promote aggregation of the 56kDa monomer using pH, solution ionic strength, etc?
ingenious device!
*promosm* ❗
So…under normal circumstances, ACE2 receptors bind to ANG2 proteins and cleaves them. SC2 spike proteins AND mRNA spike proteins ALSO bind to these sites. What happens when mRNA spikes bind to ACE2 receptors? Do spike proteins have multiple active sites for antibodies to bind to? If so, what happens when a spike protein bound to an ACE2 receptor ALSO bonds with antibodies? Will those antibodies ALSO bond to other mRNA spike proteins? If so, will this create large polymer chains of spike proteins and antibodies? Do mRNA spike proteins bind to ACE2 receptors on red blood cells? If so, does polymerization described above also happen to red blood cells? If capillary endothelial cells and red blood cells both manifest mRNA spike protein/antibody polymers, how would this affect blood flow? Where would the cell membrane > ACE2 Receptor > mRNA spike protein/antibody polymer be the weakest? What bonds would break first? Would the cell membrane tear if sufficient force is applied to the spike protein/antibody polymer? If so, would this tear open the capillary endothelium tear open of sufficient polymerization occurs on red blood cells and capillary endothelial cells? If mRNA spike proteins bind to ACE2 receptors on blood cells, and spike/antibody polymerization occurs, could red blood cells clot without conventional clotting agents, or would the polymers merely tear open the red blood cells?
What are the realistic mass ranges (especially lower limit)?
Hi Gordo, the mass range is 30kDa to 5MDa. You can find more specifications in our product data sheets on our website - www.refeyn.com.
Sorry, this is a REALLY POOR video. I am most interested in the technique. How does reflected & scattered light measure mass????
Hi Mark, thanks for your feedback! We will see how we can improve the video so it is more clear. In the meanwhile, may we suggest watching this webinar by our co-founder Prof. Philipp Kukura, that explains the principles behind mass photometry in more detail - ruclips.net/video/gEiB5NFhJ-4/видео.html In case you have any specific questions, we'd be happy to answer them - just send us an email at info@refeyn.com.
@@refeyn Much better....
tried it today- it was so easy
How to determine the partial-loaded AAV particles by using this machine? I notice that these is a overlapping part between the 'empty' peak and 'full' peak. Is that the 'peak' representing partial-loaded AAV?
Hi there! Thanks for watching our webinar. In addition to empty and full AAV capsids, you can also identify partially-filled and over-filled capsids. We will shortly be releasing an application note containing data acquired with the SamuxMP showing empty, partially-filled, and full AAVs. Perhaps more importantly, we have AUC measurement data for the same samples that confirm the data captured on the SamuxMP. All of these datasets were acquired by one of our customers. Submit your details via www.refeyn.com/contact-us and we will send you the app note once it's out!
hi Liu, where did you base?
Thank You for the video. A question, what makes the particle come and bind to the FOV at such high concentrations? Why don't they move and settle out of the FOV?
Particles will settle both in the FoV and outside the FoV. We recommend that after the experimental run, you use a fresh well for each repeat, as the FoV (and the whole well) will be mostly saturated with sample. Hope that helps!
How do you clean the cover slips in advance?
Hello, thanks for your question! For Poly-L-Lysine slides, we recommend 5 total washes with MQ H2O > 100% 2-propanol > MQ H2O > 100% 2-propanol > MQ H2O and then get rid of the water droplets remaining by using filtered air. Hope this helps!
Groundbreaking
Cool channel, going to check out some more of your videos. I think you should check SMZeus . c o m to get more social proof.
Thank you for this great video. And I wonder what's the concentration of the poly-L-lysine buffer? Thank you again.
Thanks for your question! The concentration is 0.01%. We've found that the Sigma pre-prepared solution (Ref P4707 - 50 ml) was better than preparing it ourselves from powder.
Would you recommend to sterilize them under UV-light before proceeding to further applications?
Thanks for the question! Sterilization is not necessary; we do however wash the slides with isopropanol and water before the PLL coating to remove particles, dust and oil residues from manufacturing. Here's how - ruclips.net/video/tOc6-m71FbA/видео.html