- Видео 36
- Просмотров 30 956
Institutt for biovitenskap - Universitetet i Oslo (UiO)
Норвегия
Добавлен 20 янв 2014
Institutt for biovitenskap (IBV), gir forskningsbasert undervisning og forskerutdanning av høy kvalitet innen et bredt spekter av biologien, - eksempelvis biokjemi, mikrobiologi, fysiologi, genetikk, evolusjon, økologi, bioinformatikk og bioteknologi. Våre kandidater får jobb i offentlig forvaltning og instituttsektoren, næringliv, skolevesen og akademia.
Episode 17 - "Interpreting Common PCR Problems from the Gel"
In the final episode, you’ll learn how to interpret various issues that may appear on your gel after PCR, with a focus on both PCR 1 and PCR 2. This episode will help you identify problems and decide on the best course of action to resolve them.
DNA Metabarcoding: Library preparation instructional video series:
Module 4 - Troubleshooting
Episode 17 - "Interpreting Common PCR Problems from the Gel"
DNA Metabarcoding: Library preparation instructional video series:
Module 4 - Troubleshooting
Episode 17 - "Interpreting Common PCR Problems from the Gel"
Просмотров: 130
Видео
Episode 16 - "Troubleshooting Common Problems"
Просмотров 359Месяц назад
This episode addresses common issues encountered during the PCR process, providing practical solutions for problems like inadequate DNA yields, amplification failures, and contamination. DNA Metabarcoding: Library preparation instructional video series: Module 4 - Troubleshooting Episode 16 - "Troubleshooting Common Problems"
Episode 15 - "Equimolar Pooling of Samples for Sequencing"
Просмотров 281Месяц назад
In this episode, you’ll learn how to pool your PCR products equimolarly, ensuring that each sample contributes equally to the final sequencing pool. DNA Metabarcoding: Library preparation instructional video series Module 3 - PCR 2 Episode 15 - "Equimolar Pooling of Samples for Sequencing"
Episode 14 - "Quality Control and Quantification PCR 2"
Просмотров 209Месяц назад
Learn how to perform quality control on your PCR 2 products and quantify your products using PicoGreen quantification. This episode shows how to assess DNA concentration, ensuring your samples are ready for sequencing. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 14 - "Quality Control and Quantification PCR 2"
Episode 13 - "Operating the PCR Machine for PCR 2"
Просмотров 450Месяц назад
This episode covers the setup and operation of the PCR machine for the second amplification step. Viewers will see the specific program settings used to attach barcodes to the DNA samples. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 13 - "Operating the PCR Machine for PCR 2"
Episode 12 - "Pipetting the Samples for PCR 2"
Просмотров 2,1 тыс.Месяц назад
Learn how to accurately pipette your DNA samples for the second round of PCR. This episode emphasizes the continued use of systematic tracking to ensure consistency and accuracy. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 12 - "Pipetting the Samples for PCR 2"
Episode 11 - "Preparing the Master Mix for PCR 2"
Просмотров 1,4 тыс.Месяц назад
In this episode, you’ll see the preparation of the master mix for the second round of PCR, with a focus on the increased reagent volumes needed for indexing DNA samples. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 11 - "Preparing the Master Mix for PCR 2"
Episode 10 - "Introduction to PCR 2"
Просмотров 1022 месяца назад
This episode introduces the second step of PCR, where unique barcodes are attached to DNA samples. You’ll learn about the importance of this step in preparing your samples for sequencing and how it builds on the first round of PCR. DNA Metabarcoding: Library preparation instructional video series: Module 3 - PCR 2 Episode 10 - "Introduction to PCR 2"
Episode 9 - "Magnetic bead cleaning"
Просмотров 2362 месяца назад
In this episode, you’ll learn how to purify your PCR products using magnetic beads, a crucial step to remove unwanted primerdimers and ensure your DNA samples are ready for the next phase of the process. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 9 - "Magnetic bead cleaning"
Episode 8 - "Gel electrophoresis: operating the machine"
Просмотров 1802 месяца назад
Following gel preparation, this episode demonstrates how to load your DNA samples into the gel and run the electrophoresis machine. You’ll see how to visualize the results, helping you assess the success of your PCR. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 8 - "Gel electrophoresis: operating the machine"
Episode 7 - "Gel electrophoresis: preparing the gel"
Просмотров 2362 месяца назад
This episode introduces gel electrophoresis, focusing on preparing the agarose gel to separate DNA fragments. Viewers will learn how to create the gel mold and pour the gel for effective analysis. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 7 - "Gel electrophoresis: preparing the gel"
Episode 6 - "Operating the PCR machine for PCR 1"
Просмотров 1442 месяца назад
Learn how to properly set up and operate the PCR machine for the first round of amplification. This episode covers everything about programming the thermal cycler. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 6 - "Operating the PCR machine for PCR 1"
Episode 5 - "Pipetting the samples for PCR 1: A systematic approach"
Просмотров 1422 месяца назад
This episode guides you through the careful process of adding DNA samples to the PCR tubes, using a systematic method to track progress and minimize errors during pipetting. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 5 - "Pipetting the samples for PCR 1: A systematic approach"
Episode 4 - "Pipetting the master mix for PCR 1 in the UV-cabinet"
Просмотров 3632 месяца назад
In this episode, you’ll see how to efficiently pipette the prepared master mix into PCR tubes within the sterilized UV cabinet. Emphasis is placed on maintaining a systematic approach to avoid contamination and ensure accuracy. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 4 - "Pipetting the master mix for PCR 1 in the UV-cabinet"
Episode 3 - "Preparing the master mix for PCR 1"
Просмотров 1482 месяца назад
This episode walks you through the detailed process of preparing the master mix for the first step of PCR. You’ll learn about the importance of precise measurements and the correct order of reagent addition to ensure successful DNA amplification. DNA Metabarcoding: Library preparation instructional video series: Module 2 - PCR 1 Episode 3 - "Preparing the master mix for PCR 1"
Episode 2 - "Preparing the UV-Cabinet"
Просмотров 1972 месяца назад
Episode 2 - "Preparing the UV-Cabinet"
Episode 1 - "Introduction to two-step PCR"
Просмотров 7552 месяца назад
Episode 1 - "Introduction to two-step PCR"
Lage mikroskopipreparater fra ulike materialer
Просмотров 235Год назад
Lage mikroskopipreparater fra ulike materialer
Del 6: Pipettering med mikropipette
Просмотров 1,4 тыс.2 года назад
Del 6: Pipettering med mikropipette
Del 4: Justere lysbanen Köhlers prinsipp
Просмотров 8252 года назад
Del 4: Justere lysbanen Köhlers prinsipp
Del 2: Håndtering, sittestilling og innstilling av okular
Просмотров 1,2 тыс.2 года назад
Del 2: Håndtering, sittestilling og innstilling av okular
del 1 Introduksjon til bruk av lysmikroskop
Просмотров 2,3 тыс.2 года назад
del 1 Introduksjon til bruk av lysmikroskop
Highly informative video. Please continue with more model organism lab tours.
Thank you Joseph!
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Great content. You are doing good. Will definitely come back for the next. Thumbs Up 👍
Thank you so much Ethan!