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Protein Engineering
Индия
Добавлен 30 ноя 2015
The Lectures are given by Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions along with science opinions on contemporary issues. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research and Teaching in the fields of Structure & Molecular Biology and Biochemistry, which also include Biomolecules, Proteomics, Protein Engineering and Drug Design. Dr. Singh has published over 70 research articles in international journals and carry over 20 awards and honors on his name.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (www.gbu.ac.in). These lectures will also aid preparation of competitive examinations such as UGC NET | CSIR JRF | DBT JRF | ICMR JRF etc. for aspirants.
Dr. Nagendra Singh currently works at the School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA (www.gbu.ac.in). These lectures will also aid preparation of competitive examinations such as UGC NET | CSIR JRF | DBT JRF | ICMR JRF etc. for aspirants.
Unlocking the Secrets of ICSBDD-2023 (3) | Classical Dance Performance by Sirisha | PENS#100
#ICSBDD-2023 # danceperformance #GBU
International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand success. as a token of appreciation, this video is uploaded on PENS.
International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand success. as a token of appreciation, this video is uploaded on PENS.
Просмотров: 246
Видео
Unlocking the Secrets of ICSBDD-2023 (1) | Dance Performance | Retro to Metro| PENS#98
Просмотров 579Год назад
#ICSBDD-2023 # danceperformance #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand...
International Conference on Structural Biology & Drug Discovery-2023| ICSBDD-2023 | PENS#97
Просмотров 107Год назад
#ICSBDD-2023 #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA.
NMR Spectroscopy (Part 4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96
Просмотров 959Год назад
NMR Spectroscopy (Part4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96 #nmrspectroscopy #protonexchange #nmrspectra The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#95
Просмотров 595Год назад
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#950 #nmrspectra #peaksplitting #nmrspectroscopy The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94
Просмотров 7952 года назад
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94 #nmrspectroscopy #nmrspectra #chemicalshift NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR ...
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93
Просмотров 1,3 тыс.2 года назад
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93 #nmrspectroscopy #nmr #nmrspectra NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR imaging techniq...
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92
Просмотров 4,8 тыс.2 года назад
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research an...
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91
Просмотров 1,4 тыс.2 года назад
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91 #EMSA #gelretardation #proteinDNAinteractions An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis is a typical affinity electrophoresis technique used to analyse protein-DNA or protein-RNA interactions. It is also known as a gel shift assay, gel mobility shift assay, band...
Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90
Просмотров 3,7 тыс.2 года назад
#peptidemassfingerprinting #PMF #proteinidentification Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90 Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for identifying proteins by first cleaving an unknown protein of interest into smaller peptides and then mass spectrometrically analyzing them, such...
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89
Просмотров 2,5 тыс.2 года назад
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89 #2dgel #2DGE #proteomics The primary technique for proteomics research is two-dimensional gel electrophoresis, or 2D-PAGE. It uses two different features of proteins to distinguish the complicated mixture of samples. Proteins are distinguished in the first dimension by their pI value, and in the...
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88
Просмотров 1,8 тыс.2 года назад
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88 #proteomics #genomics #proteome Proteomics is the study of a cell's, tissue's, or organism's total protein complement under a set of defined conditions. It is currently reliant on decades of technological and instrumental advancements. Advances in mass spectrometry (MS) technology, protein fractio...
Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87
Просмотров 6 тыс.2 года назад
#tandemaffinitypurification #tap #proteomics Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi...
Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86
Просмотров 3,1 тыс.2 года назад
#proteinproteininteractions #proteomics #oligomericproteins Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86 protein protein interaction techniques protein protein interaction examples protein protein interaction analysis protein protein interaction assay of protein-protein interaction importance of protein protein interaction application of protein-protein inter...
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Просмотров 5 тыс.2 года назад
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Просмотров 8342 года назад
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Просмотров 1,6 тыс.2 года назад
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
Просмотров 1,1 тыс.2 года назад
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Просмотров 9393 года назад
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Просмотров 7373 года назад
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Просмотров 1,1 тыс.3 года назад
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
Просмотров 2183 года назад
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Просмотров 7623 года назад
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
Просмотров 9883 года назад
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
Просмотров 4,2 тыс.3 года назад
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Просмотров 11 тыс.3 года назад
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
Просмотров 15 тыс.3 года назад
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Просмотров 1,1 тыс.3 года назад
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72
Просмотров 4923 года назад
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72
You just saved my life. Thaaaank youuu so much sir❤
How did he get 19.6mg
THANKS
You're welcome!
Mic lagana pdega ya .. earphone se ho jyga kuch bataya nhi
laptop ka inbuild mic bhi kam kr dega agar mic nai hai
@proteinengineering007 aur agar mic hai to kaise use krege
@@shopaholicme3909 mic plugin kro laptop me and start recording...
Tomorrow is my exam and u saved me 🥹🥹 thnx a lot . This is what i was looking for
I am glad it helped ... Thank you.
Thanks Sir 🙏🏾
Most welcome
Sir, please can you help me with this question. Suppose I am doing an enzymatic hydrolysis of feather OR chicken liver protein … how can then calculate the molecular weight of my hydrolysate ?? Thank you sir 🙏
One can run SDS-PAGE to calculate molecular weight of peptide and proteins. Although In SDS-PAGE one may miss small peptide fragments. most precise method is mass spectroscopy to detect everything after hydrolysis.
wonderful
Thank you! Cheers!
Thank you sir.
My pleasure
Thank you
Sir, can you clarify the sensitivity of Lowry, Bradford and BCA methods of protein estimation
u may visit... ruclips.net/video/DRJsCR3HMCM/видео.htmlsi=H-nr-lPnCZsdoQmh
Thank you sir❤❤
Proper process in lab?
Sir can you please explain how we know charge to mass ratio of ions from the current coming out of detector
ruclips.net/video/nKimCwkoljs/видео.htmlsi=XEgWTrNxyIOqDk42 hope it will help
Analyzer is generally pre-calibrated . eg it is known for a n analyzer what m/z ions will be collected at detector with a given magnetic field.
For 5 sample test tube, instead of water for volume make upto 1000micorliter which buffer should we add
one can use same buffer in which protein sample is dissolved.
Which diluent add in bradford reagent to dilute upto 1L
water is added to make up the volume.
Thank you so much
Are sirji lekin job hi nahi milti to kya fayda biotechnology kar ke
Very informatic vidio we got all required knowledge on bio technology thanks a lot.
Thanks sir bohat achy se samjha ya aap ny
i m glad it helped u..thanks
How BSA concentration(mg/ml) of test tube 1-5 is calculated?
Big thanks for explaining the topic in such easy manner 🫡
I am glad @harshitapandey8874 ..thank you
Very well explained 🎉
Sir msc biotech kr lene ke turant baad achi job milti h ya nhi.
In india scope h ya nhi
In india scope h ya nhi
Thank you sir it was very insightful.
Thank you so much sir 🙂
Nice information.
Very nice sir . I satisfied of your video thx 🙏🙏🙏🙏🙏🙏🙏 sir
i m glad it helped u..thanks
thanks for very useful content👌👍🙏
Sir, at 12:29, the first amino acid will superimpose exactly on the 19th amino acid and not 18th, according to the calculation.
Vry.nicely explained with detail Guidence related to Biotechnology.Thanks for elaborate Awerness.
Gracefully performed ❤
👌👌❤
Super chala chala bagundi
చాలా బాగా చేసింది
Very beautiful
Much helpful lecture sir, thanks a ton
Thank you so much Sir, very well explained in easy words .
i m glad..thank u
U all made the conference a huge success...thanks to all of you for such a wonderful and entertaining contribution...❤
🎉🎉🎉🎉🎉 too good
Indeed, a treat to eyes. 😍
Unforgettable moment ✨💕 Everything is just so perfect.
Thank you much informative
thank you
Excellent explanation - not wordy, to the point with excellent relevant examples throughout; clear and concise! Thank you Dr Singh! 👍
Thank you
C g goverment school me lekcharara ban sakte hai ya nahi
yes
Thank you for your informative video. Just I think the calculation needs some correction. If u take 0.2 ml from 1mg/ml stock solution and make 1 ml standard solution, the concentration will be 200 microliter/ml, not 20 microliter/ml. The same for the following conc
On what basis we use different detectors
depending on nature of analyte and cost available.
File ko mobile me kese send kre
connect laptop to PC and transfer ppt file as such
Sir file save kese kre please btaiye
file apne app save ho jayegi..presentation end hone ke bad
Sir i have done bsc biotechnology honours can i be pgt biology in madhya pradesh?
Biotechnology fields are the varry interesting fild