Protein Engineering
Protein Engineering
  • Видео 101
  • Просмотров 499 751
Unlocking the Secrets of ICSBDD-2023 (3) | Classical Dance Performance by Sirisha | PENS#100
#ICSBDD-2023 # danceperformance #GBU
International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand success. as a token of appreciation, this video is uploaded on PENS.
Просмотров: 235

Видео

Unlocking the Secrets of ICSBDD-2023 (1) | Dance Performance | Retro to Metro| PENS#98
Просмотров 56111 месяцев назад
#ICSBDD-2023 # danceperformance #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand...
International Conference on Structural Biology & Drug Discovery-2023| ICSBDD-2023 | PENS#97
Просмотров 10011 месяцев назад
#ICSBDD-2023 #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA.
NMR Spectroscopy (Part 4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96
Просмотров 946Год назад
NMR Spectroscopy (Part4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96 #nmrspectroscopy #protonexchange #nmrspectra The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#95
Просмотров 578Год назад
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#950 #nmrspectra #peaksplitting #nmrspectroscopy The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94
Просмотров 768Год назад
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94 #nmrspectroscopy #nmrspectra #chemicalshift NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR ...
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93
Просмотров 1,2 тыс.2 года назад
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93 #nmrspectroscopy #nmr #nmrspectra NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR imaging techniq...
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92
Просмотров 4,6 тыс.2 года назад
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research an...
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91
Просмотров 1,3 тыс.2 года назад
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91 #EMSA #gelretardation #proteinDNAinteractions An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis is a typical affinity electrophoresis technique used to analyse protein-DNA or protein-RNA interactions. It is also known as a gel shift assay, gel mobility shift assay, band...
Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90
Просмотров 3,4 тыс.2 года назад
#peptidemassfingerprinting #PMF #proteinidentification Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90 Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for identifying proteins by first cleaving an unknown protein of interest into smaller peptides and then mass spectrometrically analyzing them, such...
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89
Просмотров 2,4 тыс.2 года назад
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89 #2dgel #2DGE #proteomics The primary technique for proteomics research is two-dimensional gel electrophoresis, or 2D-PAGE. It uses two different features of proteins to distinguish the complicated mixture of samples. Proteins are distinguished in the first dimension by their pI value, and in the...
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88
Просмотров 1,8 тыс.2 года назад
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88 #proteomics #genomics #proteome Proteomics is the study of a cell's, tissue's, or organism's total protein complement under a set of defined conditions. It is currently reliant on decades of technological and instrumental advancements. Advances in mass spectrometry (MS) technology, protein fractio...
Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87
Просмотров 6 тыс.2 года назад
#tandemaffinitypurification #tap #proteomics Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi...
Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86
Просмотров 2,9 тыс.2 года назад
#proteinproteininteractions #proteomics #oligomericproteins Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86 protein protein interaction techniques protein protein interaction examples protein protein interaction analysis protein protein interaction assay of protein-protein interaction importance of protein protein interaction application of protein-protein inter...
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Просмотров 5 тыс.2 года назад
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Просмотров 7552 года назад
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Просмотров 1,5 тыс.2 года назад
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
Просмотров 9642 года назад
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Просмотров 9203 года назад
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Просмотров 7293 года назад
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Просмотров 1,1 тыс.3 года назад
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
Просмотров 2163 года назад
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Просмотров 7433 года назад
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
Просмотров 9623 года назад
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
Просмотров 4 тыс.3 года назад
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Просмотров 11 тыс.3 года назад
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
Просмотров 15 тыс.3 года назад
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Просмотров 1 тыс.3 года назад
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72
Просмотров 4873 года назад
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72

Комментарии

  • @pauletteopitz8581
    @pauletteopitz8581 28 дней назад

    Sir, please can you help me with this question. Suppose I am doing an enzymatic hydrolysis of feather OR chicken liver protein … how can then calculate the molecular weight of my hydrolysate ?? Thank you sir 🙏

    • @proteinengineering007
      @proteinengineering007 15 дней назад

      One can run SDS-PAGE to calculate molecular weight of peptide and proteins. Although In SDS-PAGE one may miss small peptide fragments. most precise method is mass spectroscopy to detect everything after hydrolysis.

  • @subhrasankardutta6010
    @subhrasankardutta6010 Месяц назад

    wonderful

  • @mathiasmwango4144
    @mathiasmwango4144 Месяц назад

    Thank you sir.

  • @dancepoetry1456
    @dancepoetry1456 Месяц назад

    Thank you

  • @sandeepsaini5340
    @sandeepsaini5340 2 месяца назад

    Sir, can you clarify the sensitivity of Lowry, Bradford and BCA methods of protein estimation

    • @proteinengineering007
      @proteinengineering007 Месяц назад

      u may visit... ruclips.net/video/DRJsCR3HMCM/видео.htmlsi=H-nr-lPnCZsdoQmh

  • @Dr.sheetal..998
    @Dr.sheetal..998 2 месяца назад

    Thank you sir❤❤

  • @Khanniazi-f6l
    @Khanniazi-f6l 2 месяца назад

    Proper process in lab?

  • @vikki10pavam25
    @vikki10pavam25 3 месяца назад

    Sir can you please explain how we know charge to mass ratio of ions from the current coming out of detector

    • @proteinengineering007
      @proteinengineering007 3 месяца назад

      ruclips.net/video/nKimCwkoljs/видео.htmlsi=XEgWTrNxyIOqDk42 hope it will help

    • @proteinengineering007
      @proteinengineering007 3 месяца назад

      Analyzer is generally pre-calibrated . eg it is known for a n analyzer what m/z ions will be collected at detector with a given magnetic field.

  • @drrchavan7011
    @drrchavan7011 3 месяца назад

    For 5 sample test tube, instead of water for volume make upto 1000micorliter which buffer should we add

    • @proteinengineering007
      @proteinengineering007 3 месяца назад

      one can use same buffer in which protein sample is dissolved.

  • @drrchavan7011
    @drrchavan7011 3 месяца назад

    Which diluent add in bradford reagent to dilute upto 1L

  • @Atulbhai362
    @Atulbhai362 3 месяца назад

    Are sirji lekin job hi nahi milti to kya fayda biotechnology kar ke

  • @satyanarayanadevalla6620
    @satyanarayanadevalla6620 4 месяца назад

    Very informatic vidio we got all required knowledge on bio technology thanks a lot.

  • @aarzooarshi4767
    @aarzooarshi4767 4 месяца назад

    Thanks sir bohat achy se samjha ya aap ny

  • @mulhimmukhtar1474
    @mulhimmukhtar1474 4 месяца назад

    How BSA concentration(mg/ml) of test tube 1-5 is calculated?

  • @Atittudeboy124
    @Atittudeboy124 4 месяца назад

    2024 𝚖𝚎 𝚔𝚎𝚢𝚊 𝚑𝚎

  • @harshitapandey8874
    @harshitapandey8874 4 месяца назад

    Big thanks for explaining the topic in such easy manner 🫡

  • @KamaldeepKaur-v6x
    @KamaldeepKaur-v6x 6 месяцев назад

    Very well explained 🎉

  • @shreyajaiswal3550
    @shreyajaiswal3550 6 месяцев назад

    Sir msc biotech kr lene ke turant baad achi job milti h ya nhi.

  • @yashdeepsingh1790
    @yashdeepsingh1790 7 месяцев назад

    Thank you sir it was very insightful.

  • @shree_12
    @shree_12 8 месяцев назад

    Thank you so much sir 🙂

  • @pradeepbhatnagar4848
    @pradeepbhatnagar4848 8 месяцев назад

    Nice information.

  • @mamtagupta-tj9by
    @mamtagupta-tj9by 8 месяцев назад

    Very nice sir . I satisfied of your video thx 🙏🙏🙏🙏🙏🙏🙏 sir

  • @dpcbasti9904
    @dpcbasti9904 10 месяцев назад

    thanks for very useful content👌👍🙏

  • @kanksha_koumudee
    @kanksha_koumudee 10 месяцев назад

    Sir, at 12:29, the first amino acid will superimpose exactly on the 19th amino acid and not 18th, according to the calculation.

  • @pradeepbhatnagar4848
    @pradeepbhatnagar4848 11 месяцев назад

    Vry.nicely explained with detail Guidence related to Biotechnology.Thanks for elaborate Awerness.

  • @padmasavi
    @padmasavi 11 месяцев назад

    Gracefully performed ❤

  • @phaniganesh4018
    @phaniganesh4018 11 месяцев назад

    👌👌❤

  • @bhamidibhadramma769
    @bhamidibhadramma769 11 месяцев назад

    Super chala chala bagundi

  • @kameswariparupudi2148
    @kameswariparupudi2148 11 месяцев назад

    చాలా బాగా చేసింది

  • @RashmiJoshi-f8m
    @RashmiJoshi-f8m 11 месяцев назад

    Very beautiful

  • @subsstation2418
    @subsstation2418 11 месяцев назад

    Much helpful lecture sir, thanks a ton

  • @Nabila-l8n
    @Nabila-l8n 11 месяцев назад

    Thank you so much Sir, very well explained in easy words .

  • @nagendrasingh658
    @nagendrasingh658 11 месяцев назад

    U all made the conference a huge success...thanks to all of you for such a wonderful and entertaining contribution...❤

  • @S-ck1cz
    @S-ck1cz 11 месяцев назад

    🎉🎉🎉🎉🎉 too good

  • @SwaraSingh-in1yk
    @SwaraSingh-in1yk 11 месяцев назад

    Indeed, a treat to eyes. 😍

  • @SwaraSingh-in1yk
    @SwaraSingh-in1yk 11 месяцев назад

    Unforgettable moment ✨💕 Everything is just so perfect.

  • @zeniarose8110
    @zeniarose8110 Год назад

    Thank you much informative

  • @flowerpetal6193
    @flowerpetal6193 Год назад

    Excellent explanation - not wordy, to the point with excellent relevant examples throughout; clear and concise! Thank you Dr Singh! 👍

  • @GhanshyamSahu-q9f
    @GhanshyamSahu-q9f Год назад

    C g goverment school me lekcharara ban sakte hai ya nahi

  • @ahmadmola9574
    @ahmadmola9574 Год назад

    Thank you for your informative video. Just I think the calculation needs some correction. If u take 0.2 ml from 1mg/ml stock solution and make 1 ml standard solution, the concentration will be 200 microliter/ml, not 20 microliter/ml. The same for the following conc

  • @attahussain2643
    @attahussain2643 Год назад

    On what basis we use different detectors

  • @SASStudypoint.
    @SASStudypoint. Год назад

    File ko mobile me kese send kre

  • @SASStudypoint.
    @SASStudypoint. Год назад

    Sir file save kese kre please btaiye

  • @sudeshkaushal7999
    @sudeshkaushal7999 Год назад

    Sir i have done bsc biotechnology honours can i be pgt biology in madhya pradesh?

  • @prashantchaudhari2952
    @prashantchaudhari2952 Год назад

    Biotechnology fields are the varry interesting fild

  • @foodtechnologist838
    @foodtechnologist838 Год назад

    Kindly reply me sir..is sodium potassium tartrate and potassium sodium tartrate same?? Because I have potassium sodium tartrate.

    • @AREEBA_aa
      @AREEBA_aa 6 месяцев назад

      yes is is obvious....

  • @tahirasaleem2794
    @tahirasaleem2794 Год назад

    😊

  • @tahirasaleem2794
    @tahirasaleem2794 Год назад

    V informative

  • @nikitatailor8679
    @nikitatailor8679 Год назад

    Sir MSc biotechnology m krne k bad private ya govt hospital m work kr skate h kyaa lab m

  • @shreechannel9183
    @shreechannel9183 Год назад

    Thank thank u so much sir very useful video