Protein Engineering
Protein Engineering
  • Видео 101
  • Просмотров 510 626
Unlocking the Secrets of ICSBDD-2023 (3) | Classical Dance Performance by Sirisha | PENS#100
#ICSBDD-2023 # danceperformance #GBU
International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand success. as a token of appreciation, this video is uploaded on PENS.
Просмотров: 246

Видео

Unlocking the Secrets of ICSBDD-2023 (1) | Dance Performance | Retro to Metro| PENS#98
Просмотров 579Год назад
#ICSBDD-2023 # danceperformance #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA. My students performed in the cultural program to showcase their extracurricular talent. These are all UG/PG students who worked tirelessly to make ICSBDD a grand...
International Conference on Structural Biology & Drug Discovery-2023| ICSBDD-2023 | PENS#97
Просмотров 107Год назад
#ICSBDD-2023 #GBU International Conference on Structural Biology and Drug Discovery (ICSBDD) 11-12 October 2023 was organized by the University School of Biotechnology, Gautam Buddha University, Greater Noida, INDIA.
NMR Spectroscopy (Part 4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96
Просмотров 959Год назад
NMR Spectroscopy (Part4) | Proton Exchange | Deuterium in H1 NMR | Dr. Nagendra Singh | PENS#96 #nmrspectroscopy #protonexchange #nmrspectra The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#95
Просмотров 595Год назад
NMR Spectroscopy (Part 3) | Mechanism of Signal Splitting in NMR | Dr. Nagendra Singh | PENS#950 #nmrspectra #peaksplitting #nmrspectroscopy The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) Ne...
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94
Просмотров 7952 года назад
NMR Spectroscopy (Part 2) | NMR Spectra | Chemical Shift || Dr. Nagendra Singh | PENS#94 #nmrspectroscopy #nmrspectra #chemicalshift NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR ...
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93
Просмотров 1,3 тыс.2 года назад
NMR Spectroscopy (Part 1) | Basic Principle | H1 NMR | Dr. Nagendra Singh | PENS#93 #nmrspectroscopy #nmr #nmrspectra NMR spectroscopy is the use of NMR phenomena to study the physical, chemical, and biological properties of matter. Chemists use it to determine molecular identity and structure. Medical practitioners employ magnetic resonance imaging (MRI), a multidimensional NMR imaging techniq...
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92
Просмотров 4,8 тыс.2 года назад
GST Pull Down Assay & Co Immunoprecipitation | Protein-Protein Interactions | PENS#92 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi. Dr. Singh has a vast experience of research an...
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91
Просмотров 1,4 тыс.2 года назад
Electrophoretic Mobility Shift Assay | EMSA | Gel Retardation Assay | Gel Shift Assay | PENS#91 #EMSA #gelretardation #proteinDNAinteractions An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis is a typical affinity electrophoresis technique used to analyse protein-DNA or protein-RNA interactions. It is also known as a gel shift assay, gel mobility shift assay, band...
Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90
Просмотров 3,7 тыс.2 года назад
#peptidemassfingerprinting #PMF #proteinidentification Peptide Mass Fingerprinting | Protein Fingerprinting | PMF | Protein Identification | PENS#90 Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for identifying proteins by first cleaving an unknown protein of interest into smaller peptides and then mass spectrometrically analyzing them, such...
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89
Просмотров 2,5 тыс.2 года назад
2D Gel Electrophoresis| Principle & Limitations | 2 Dimensional Gel Electrophoresis | 2DGE | PENS#89 #2dgel #2DGE #proteomics The primary technique for proteomics research is two-dimensional gel electrophoresis, or 2D-PAGE. It uses two different features of proteins to distinguish the complicated mixture of samples. Proteins are distinguished in the first dimension by their pI value, and in the...
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88
Просмотров 1,8 тыс.2 года назад
Introduction to Proteomics | Proteomics and Genomics | Aims and Objectives of Proteomics | PENS#88 #proteomics #genomics #proteome Proteomics is the study of a cell's, tissue's, or organism's total protein complement under a set of defined conditions. It is currently reliant on decades of technological and instrumental advancements. Advances in mass spectrometry (MS) technology, protein fractio...
Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87
Просмотров 6 тыс.2 года назад
#tandemaffinitypurification #tap #proteomics Tandem Affinity Purification (TAP) | Protein-Protein Interactions | Techniques | PENS#87 The Lectures are given Dr. Nagendra Singh aiming to spread basic understanding and awareness of biological molecules and reactions. Dr. Nagendra Singh finished his Doctoral and Post Doctoral studies from "All India Institute of Medical Sciences" (AIIMS) New Delhi...
Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86
Просмотров 3,1 тыс.2 года назад
#proteinproteininteractions #proteomics #oligomericproteins Protein - Protein Interactions | PPI | Techniques | Dr. Nagendra Singh | PENS#86 protein protein interaction techniques protein protein interaction examples protein protein interaction analysis protein protein interaction assay of protein-protein interaction importance of protein protein interaction application of protein-protein inter...
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Просмотров 5 тыс.2 года назад
Sequential and Ping Pong Reactions Mechanism | Bi-Substrate Enzyme Reactions | PENS#85
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Просмотров 8342 года назад
Edman Degradation (Part 2) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#84
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Просмотров 1,6 тыс.2 года назад
Edman Degradation (Part 1) | Edman Sequencing Method | Amino Acid Sequencing | Limitations | PENS#83
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
Просмотров 1,1 тыс.2 года назад
Alpha Helix | Protein Structure | Numerical Questions | PENS#82
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Просмотров 9393 года назад
UV Visible Spectroscopy (Hindi) | Spectrophotometer | Empirical Rules| Dr. Nagendra Singh | PENS#81
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Просмотров 7373 года назад
Mole Concept (PART 2) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#80
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Просмотров 1,1 тыс.3 года назад
Mole Concept (PART 1) | Molarity | Numerical Questions | UGC NET | Dr. Nagendra Singh | PENS#79
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
Просмотров 2183 года назад
Easy ways of Online Teaching | Teaching with PPT and PDF | Dr. Nagendra Singh | PENS#78
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Просмотров 7623 года назад
How to Make an Effective Power Point Presentation (Hindi) | PPT Rules | Dr. Nagendra Singh | PENS#77
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
Просмотров 9883 года назад
Part Per Million (PPM) | PPB | PPT| Numerical Questions | Dr. Nagendra Singh | PENS#76
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
Просмотров 4,2 тыс.3 года назад
ESI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry | Dr. Nagendra Singh | PENS#75
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Просмотров 11 тыс.3 года назад
MALDI TOF Mass Spectrometry | Deconvolution of Mass Spectra | Dr. Nagendra Singh | PENS#74
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
Просмотров 15 тыс.3 года назад
Ion Detectors in Mass Spectrometry | Faraday Cup| Electron Multipliers| Dr. Nagendra Singh | PENS#73
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Просмотров 1,1 тыс.3 года назад
UV Vis Spectroscopy | Principle and Applications | Empirical Rules | Structural studies | PENS#81
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72
Просмотров 4923 года назад
Best COVID-19 Vaccine | Mechanism of Action | COVID 19 Vaccine Design | Dr. Nagendra Singh | PENS#72

Комментарии

  • @Thaber-m7n
    @Thaber-m7n 14 дней назад

    You just saved my life. Thaaaank youuu so much sir❤

  • @skipperdhar2437
    @skipperdhar2437 15 дней назад

    How did he get 19.6mg

  • @tayssirsalem875
    @tayssirsalem875 22 дня назад

    THANKS

  • @shopaholicme3909
    @shopaholicme3909 27 дней назад

    Mic lagana pdega ya .. earphone se ho jyga kuch bataya nhi

    • @proteinengineering007
      @proteinengineering007 25 дней назад

      laptop ka inbuild mic bhi kam kr dega agar mic nai hai

    • @shopaholicme3909
      @shopaholicme3909 25 дней назад

      @proteinengineering007 aur agar mic hai to kaise use krege

    • @proteinengineering007
      @proteinengineering007 24 дня назад

      @@shopaholicme3909 mic plugin kro laptop me and start recording...

  • @kirtijindal9180
    @kirtijindal9180 Месяц назад

    Tomorrow is my exam and u saved me 🥹🥹 thnx a lot . This is what i was looking for

  • @anjup7312
    @anjup7312 Месяц назад

    Thanks Sir 🙏🏾

  • @pauletteopitz8581
    @pauletteopitz8581 3 месяца назад

    Sir, please can you help me with this question. Suppose I am doing an enzymatic hydrolysis of feather OR chicken liver protein … how can then calculate the molecular weight of my hydrolysate ?? Thank you sir 🙏

    • @proteinengineering007
      @proteinengineering007 2 месяца назад

      One can run SDS-PAGE to calculate molecular weight of peptide and proteins. Although In SDS-PAGE one may miss small peptide fragments. most precise method is mass spectroscopy to detect everything after hydrolysis.

  • @subhrasankardutta6010
    @subhrasankardutta6010 3 месяца назад

    wonderful

  • @mathiasmwango4144
    @mathiasmwango4144 3 месяца назад

    Thank you sir.

  • @dancepoetry1456
    @dancepoetry1456 3 месяца назад

    Thank you

  • @sandeepsaini5340
    @sandeepsaini5340 4 месяца назад

    Sir, can you clarify the sensitivity of Lowry, Bradford and BCA methods of protein estimation

    • @proteinengineering007
      @proteinengineering007 4 месяца назад

      u may visit... ruclips.net/video/DRJsCR3HMCM/видео.htmlsi=H-nr-lPnCZsdoQmh

  • @Dr.sheetal..998
    @Dr.sheetal..998 4 месяца назад

    Thank you sir❤❤

  • @Khanniazi-f6l
    @Khanniazi-f6l 4 месяца назад

    Proper process in lab?

  • @vikki10pavam25
    @vikki10pavam25 5 месяцев назад

    Sir can you please explain how we know charge to mass ratio of ions from the current coming out of detector

    • @proteinengineering007
      @proteinengineering007 5 месяцев назад

      ruclips.net/video/nKimCwkoljs/видео.htmlsi=XEgWTrNxyIOqDk42 hope it will help

    • @proteinengineering007
      @proteinengineering007 5 месяцев назад

      Analyzer is generally pre-calibrated . eg it is known for a n analyzer what m/z ions will be collected at detector with a given magnetic field.

  • @drrchavan7011
    @drrchavan7011 5 месяцев назад

    For 5 sample test tube, instead of water for volume make upto 1000micorliter which buffer should we add

    • @proteinengineering007
      @proteinengineering007 5 месяцев назад

      one can use same buffer in which protein sample is dissolved.

  • @drrchavan7011
    @drrchavan7011 5 месяцев назад

    Which diluent add in bradford reagent to dilute upto 1L

  • @Atulbhai362
    @Atulbhai362 5 месяцев назад

    Are sirji lekin job hi nahi milti to kya fayda biotechnology kar ke

  • @satyanarayanadevalla6620
    @satyanarayanadevalla6620 6 месяцев назад

    Very informatic vidio we got all required knowledge on bio technology thanks a lot.

  • @aarzooarshi4767
    @aarzooarshi4767 6 месяцев назад

    Thanks sir bohat achy se samjha ya aap ny

  • @mulhimmukhtar1474
    @mulhimmukhtar1474 6 месяцев назад

    How BSA concentration(mg/ml) of test tube 1-5 is calculated?

  • @harshitapandey8874
    @harshitapandey8874 6 месяцев назад

    Big thanks for explaining the topic in such easy manner 🫡

  • @KamaldeepKaur-v6x
    @KamaldeepKaur-v6x 8 месяцев назад

    Very well explained 🎉

  • @shreyajaiswal3550
    @shreyajaiswal3550 8 месяцев назад

    Sir msc biotech kr lene ke turant baad achi job milti h ya nhi.

  • @yashdeepsingh1790
    @yashdeepsingh1790 9 месяцев назад

    Thank you sir it was very insightful.

  • @shree_12
    @shree_12 10 месяцев назад

    Thank you so much sir 🙂

  • @pradeepbhatnagar4848
    @pradeepbhatnagar4848 10 месяцев назад

    Nice information.

  • @mamtagupta-tj9by
    @mamtagupta-tj9by 10 месяцев назад

    Very nice sir . I satisfied of your video thx 🙏🙏🙏🙏🙏🙏🙏 sir

  • @dpcbasti9904
    @dpcbasti9904 Год назад

    thanks for very useful content👌👍🙏

  • @kanksha_koumudee
    @kanksha_koumudee Год назад

    Sir, at 12:29, the first amino acid will superimpose exactly on the 19th amino acid and not 18th, according to the calculation.

  • @pradeepbhatnagar4848
    @pradeepbhatnagar4848 Год назад

    Vry.nicely explained with detail Guidence related to Biotechnology.Thanks for elaborate Awerness.

  • @padmasavi
    @padmasavi Год назад

    Gracefully performed ❤

  • @phaniganesh4018
    @phaniganesh4018 Год назад

    👌👌❤

  • @bhamidibhadramma769
    @bhamidibhadramma769 Год назад

    Super chala chala bagundi

  • @kameswariparupudi2148
    @kameswariparupudi2148 Год назад

    చాలా బాగా చేసింది

  • @RashmiJoshi-f8m
    @RashmiJoshi-f8m Год назад

    Very beautiful

  • @subsstation2418
    @subsstation2418 Год назад

    Much helpful lecture sir, thanks a ton

  • @Nabila-l8n
    @Nabila-l8n Год назад

    Thank you so much Sir, very well explained in easy words .

  • @nagendrasingh658
    @nagendrasingh658 Год назад

    U all made the conference a huge success...thanks to all of you for such a wonderful and entertaining contribution...❤

  • @S-ck1cz
    @S-ck1cz Год назад

    🎉🎉🎉🎉🎉 too good

  • @SwaraSingh-in1yk
    @SwaraSingh-in1yk Год назад

    Indeed, a treat to eyes. 😍

  • @SwaraSingh-in1yk
    @SwaraSingh-in1yk Год назад

    Unforgettable moment ✨💕 Everything is just so perfect.

  • @zeniarose8110
    @zeniarose8110 Год назад

    Thank you much informative

  • @flowerpetal6193
    @flowerpetal6193 Год назад

    Excellent explanation - not wordy, to the point with excellent relevant examples throughout; clear and concise! Thank you Dr Singh! 👍

  • @GhanshyamSahu-q9f
    @GhanshyamSahu-q9f Год назад

    C g goverment school me lekcharara ban sakte hai ya nahi

  • @ahmadmola9574
    @ahmadmola9574 Год назад

    Thank you for your informative video. Just I think the calculation needs some correction. If u take 0.2 ml from 1mg/ml stock solution and make 1 ml standard solution, the concentration will be 200 microliter/ml, not 20 microliter/ml. The same for the following conc

  • @attahussain2643
    @attahussain2643 Год назад

    On what basis we use different detectors

  • @SASStudypoint.
    @SASStudypoint. Год назад

    File ko mobile me kese send kre

  • @SASStudypoint.
    @SASStudypoint. Год назад

    Sir file save kese kre please btaiye

  • @sudeshkaushal7999
    @sudeshkaushal7999 Год назад

    Sir i have done bsc biotechnology honours can i be pgt biology in madhya pradesh?

  • @prashantchaudhari2952
    @prashantchaudhari2952 Год назад

    Biotechnology fields are the varry interesting fild