Rohini Patil
Rohini Patil
  • Видео 65
  • Просмотров 51 158
Western Blotting
Western Blotting is a technique to screen the cDNA library. It allows selection on the basis of expressed protein detection.
Просмотров: 14

Видео

Dot and Slot Blot
Просмотров 172 месяца назад
Dot blot and slot blot are technique to identify recombinant. It does not involve electrophoresis.
Colony Hybridization and Plaque Lift Assay
Просмотров 343 месяца назад
Colony hybridization and plaque lift assay are screening methods. The methods involve in situ hybridization. You may contact patilrohini194@gmail.com
Northern Blotting/ Hybridization
Просмотров 723 месяца назад
The method used to detect the RNA by blotting technique is known as Northern blot.
Blotting Techniques
Просмотров 243 месяца назад
Blotting is transfer of nucleic acid to nylon or nitrocellulose paper. The technique is utilized in southern , northern, western etc. blotting/ hybridization.
Southernwestern Blotting
Просмотров 363 месяца назад
Southernwestern Blotting technique is for identification of DNA-protein interaction.
Optimization of Codon/Codon Choice,mRNA structure:Factor Affecting cloned gene Expression in E. coli
Просмотров 289Год назад
The codon optimization is important for high level expression of foreign gene with accuracy. At the same time mRNA structure is also affecting expression of gene is discussed. You may go through Plasmid stability and Plasmid copy number ruclips.net/video/-NsQqalAwwU/видео.html Optimization of translational initiation sequence ruclips.net/video/hZssThZCG3M/видео.html Transcriptional termination ...
Plasmid stability and plasmid copy number:Structural and segregative stability,incompatibility
Просмотров 391Год назад
Plasmid copy number of vector affects expression of gene. The vector with high stability has to be selected for stable gene expression of foreign gene in E.coli host. You may go through: Promoter strength ruclips.net/video/I_MC4fizMEY/видео.html E.coli expression vector:cloned gene under controllable promoter ruclips.net/video/13DGQN3yHEk/видео.html Optimization of translation initiation sequen...
Optimization of Translational initiation sequences 5՚UTR for cloned gene in E.coli
Просмотров 103Год назад
Translational initiation sequences affect rate of translation. The SD sequence and other factors are discussed in detail. Transcriptional Termination choice for Expression Vector: ruclips.net/video/hFf-L8VLEhU/видео.html Promoter Strength: ruclips.net/video/I_MC4fizMEY/видео.html E. coli expression vector: Cloned gene under control of regulated promoter: ruclips.net/video/13DGQN3yHEk/видео.html...
Transcriptional Termination Choice for expression Vectors in E.coli
Просмотров 99Год назад
One of factor affecting expression of foreign gene E.coli is transcriptional termination site. You may contact rohini.coaching@gmail.com
Promoter Strength:lacUV5,hybrid promoters:tac,tic:factors affecting cloned gene expression in E.coli
Просмотров 586Год назад
For efficient expression of foreign genes in E.coli host promoter is required. in this video the promoters for expression of genes are discussed. Watch more about regulated promoters of E.coli expression vector ruclips.net/video/13DGQN3yHEk/видео.html You may contact rohini.coaching@gmail.com
Response element :Eukaryotic Gene Expression Regulation
Просмотров 246Год назад
Response elements are cis acting element. Response element are activator binding site. You may contact rohini.coaching@gmail.com
Difference between pUC18 and pUC19/Compare and Contrast:pUC18 and pUC19/high copy no. plasmid vector
Просмотров 945Год назад
The discussion is regarding similarities and dissimilarities between pUC18 and pUC19 vectors. In detail focuses on characteristics of pUC18 and pUC19 vectors. You may contact rohini.coaching@gmail.com
Alpha Complementation, Blue White selection, pUC18
Просмотров 235Год назад
Alpha complementation can be used as selection or screening technique. It can be used to select recombinant. You my contact rohini.coaching@gmail.com
Two Hybrid System, Two Hybrid Assay,Yeast Hybrid System,Method to detect protein protein interaction
Просмотров 115Год назад
Two hybrid assay or two hybrid system is used to detect protein protein interaction. The video explains in detail how is assay carried out. You may contact rohini.coaching@gmail.com
DNA Binding Domain, Activating Region: Zinc Finger,Leucine Zipper,Helix Loop Helix,Helix Turn Helix
Просмотров 882Год назад
DNA Binding Domain, Activating Region: Zinc Finger,Leucine Zipper,Helix Loop Helix,Helix Turn Helix
E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
Просмотров 1,8 тыс.Год назад
E.coli Expression Vectors: Cloned Gene Under Control of Regulated Promoter: λPL,T7, pBAD, pET vector
Global Regulators/Global control system/Global control Network/Global Regulatory System
Просмотров 357Год назад
Global Regulators/Global control system/Global control Network/Global Regulatory System
gal operon: Structure, Regulation, two levels of regulation, GalR
Просмотров 2,1 тыс.Год назад
gal operon: Structure, Regulation, two levels of regulation, GalR
Isolation of Bacterial Genomic DNA: Steps for isolation, methods to purify and concentrate DNA
Просмотров 1822 года назад
Isolation of Bacterial Genomic DNA: Steps for isolation, methods to purify and concentrate DNA
Finding Concentration of DNA and Purity Check of Isolated DNA
Просмотров 1822 года назад
Finding Concentration of DNA and Purity Check of Isolated DNA
Plasmid DNA Isolation Methods:Alkaline lysis, EtBr-CsCl density gradient, Isolation on basis of Size
Просмотров 1 тыс.2 года назад
Plasmid DNA Isolation Methods:Alkaline lysis, EtBr-CsCl density gradient, Isolation on basis of Size
Real time PCR/ Quantitative PCR/ RT-PCR/ qPCR
Просмотров 522 года назад
Real time PCR/ Quantitative PCR/ RT-PCR/ qPCR
Inverse PCR : Variation of PCR / Types of PCR
Просмотров 2162 года назад
Inverse PCR : Variation of PCR / Types of PCR
Primer Designing for PCR
Просмотров 532 года назад
Primer Designing for PCR
Polymerase Chain Reaction/PCR Basic Principle/ Essential Components of PCR/Parameters Affecting PCR
Просмотров 1392 года назад
Polymerase Chain Reaction/PCR Basic Principle/ Essential Components of PCR/Parameters Affecting PCR
Genetic / Genomic Imprinting: Importance in Epigenetics, When and How imprinting happens: Disorders
Просмотров 5002 года назад
Genetic / Genomic Imprinting: Importance in Epigenetics, When and How imprinting happens: Disorders
No Go Decay pathway: Dom34/Hbs1, Pelota/Hbs1, Pelota in Begomovirus resistance in Tomato, ty5 locus
Просмотров 2342 года назад
No Go Decay pathway: Dom34/Hbs1, Pelota/Hbs1, Pelota in Begomovirus resistance in Tomato, ty5 locus
Chromatin remodeling complexes/Antirepressor, HO gene & PHO5 gene Activation
Просмотров 4882 года назад
Chromatin remodeling complexes/Antirepressor, HO gene & PHO5 gene Activation
Histone Modification: Acetylation, Deacetylation, Methylation Demethylation of Histones
Просмотров 3892 года назад
Histone Modification: Acetylation, Deacetylation, Methylation Demethylation of Histones

Комментарии

  • @rakxhikaasingh8157
    @rakxhikaasingh8157 13 дней назад

    out of so many videos about the same topic this one finally made me clear the concept. Thanks ma'am .

  • @عباسعلىعنادالبعاج

    plz . i couldnt find the previous lecture about integron .

    • @rohinipatil6606
      @rohinipatil6606 29 дней назад

      You will find it in drug resistant play list

  • @crayversace2804
    @crayversace2804 2 месяца назад

    So technically the integron integrate gene isn’t really mobile, it’s just a gene cassette that can add and remove ARGs. The real mobile genetic elements here are transposons

  • @soumavomondal6079
    @soumavomondal6079 4 месяца назад

    Can you explain what is derepression of gcn4

    • @rohinipatil6606
      @rohinipatil6606 4 месяца назад

      Depression means removal of repression and to get activated. Now in case of Gcn4, translation of Gcn4 is blocked that is there is repressed condition in high concentration of amino acids. When concentration of amino acid is low the block i.e. repression is removed. It is derepression of Gcn4.

  • @karinaburgess3989
    @karinaburgess3989 5 месяцев назад

    I dont understand how when glactose is being converted to carbon and energy you said the sequence of events was GALACTOSE -> UDP GLUCOSE -> GLU.1.PHOSPHATE-> GLYCOLYSIS When the diagrams show that galactose needs to be converted into GLU.1.PH before UDP-GALACTOSE and then UDP GLUCOSE

    • @karinaburgess3989
      @karinaburgess3989 5 месяцев назад

      Otherwise thank you amazing video really helps me understand !!

    • @rohinipatil6606
      @rohinipatil6606 3 месяца назад

      Sequence of event is gal to gal-1p to gal-udp to glucose -udp. Glucose -UDP enters glycolysis to give energy.

  • @maya6858
    @maya6858 7 месяцев назад

    What about complex transposons

  • @pankajpandey3247
    @pankajpandey3247 9 месяцев назад

    please make a video on serial dilution and bacterial cell counting

  • @kawaikowaiwegath8426
    @kawaikowaiwegath8426 9 месяцев назад

    Thank you so much ma'am now I really understand. My lecturer failed to do this❤😢

  • @backup3412
    @backup3412 9 месяцев назад

    One of the best videos of all time!

  • @annafiarnob
    @annafiarnob 9 месяцев назад

    Thanks a lot 💚your understanding method was easy and sharp

  • @meghnaghosal1051
    @meghnaghosal1051 10 месяцев назад

    If eif 2 is phosphorylated when amino acid is at lower conc , how will it aid other charger trna on the codon . As phosphorylated form of eif2 has higher affinity with gdp and in gdp form its inactive then how will it be continue translation of main orf of gcn4

    • @rohinipatil6606
      @rohinipatil6606 9 месяцев назад

      Remember that not all molecules are phosphorylated at any time.

  • @lanchandanchan2652
    @lanchandanchan2652 10 месяцев назад

    Kash yehi lecture clg mey karliye hotey too ajj ye nahi dekhney padey hotey 😅

  • @aakashmore9822
    @aakashmore9822 10 месяцев назад

    Thank you mam ❤

  • @prashikganvir6140
    @prashikganvir6140 10 месяцев назад

    Thank you mam Lord Buddha bless u

  • @azadbharath7444
    @azadbharath7444 10 месяцев назад

    Substantial 🎉TQ mam.

  • @shrutisalunke1704
    @shrutisalunke1704 11 месяцев назад

    ma'am , Is that etf2 or eif2 ?

  • @vijetagange
    @vijetagange 11 месяцев назад

    Nice explanation mam💢

  • @AmritKaur-cz4yz
    @AmritKaur-cz4yz 11 месяцев назад

    Thanks mam

  • @shrutisalunke1704
    @shrutisalunke1704 11 месяцев назад

    Thank you ma'am for the detailed explanation. I understood all your video lectures.

  • @pankajpandey3247
    @pankajpandey3247 Год назад

    Very recently I came to know about your channel. The videos are very informative. I have a query.......How we can ensure that there is no plasmid contamination during bacterial DNA extraction?

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      A separate band of plasmid and genomic DNA is visible on agarose gel electrophoresis.

    • @pankajpandey3247
      @pankajpandey3247 Год назад

      Sorry to bother.....one more query.... What we can do to avoid plasmid extraction during bacterial DNA extraction

  • @chandanachandu6064
    @chandanachandu6064 Год назад

    Mam please do vedios on computational biology , structural and functional and comparative genomics

  • @ishadahale538
    @ishadahale538 Год назад

    Thank you so much mam🙏🙏

  • @LaxmiPrasanna-h9q
    @LaxmiPrasanna-h9q Год назад

    Thank you for the explanation madam...

  • @revolution515
    @revolution515 Год назад

    Very nicely explained Madam. धन्यवाद

  • @NITISHKUMAR-un2no
    @NITISHKUMAR-un2no Год назад

    My favorite teacher of mol bio

  • @gayatrikuwar4545
    @gayatrikuwar4545 Год назад

    It helped me a lot Thankyu ❤

  • @shahbazmalik670
    @shahbazmalik670 Год назад

    ThanKs maam ❤

  • @aj_patil.
    @aj_patil. Год назад

    Plz in Hindi videos

  • @vikramgaikwad2599
    @vikramgaikwad2599 Год назад

    Very helpful Thank you!

  • @saumyaprachi2858
    @saumyaprachi2858 Год назад

    I understood it perfectly ☺️ Thank you so much ❤️✨

  • @chanderparkashbalodiya3063
    @chanderparkashbalodiya3063 Год назад

    Very nice explanation, Thank you maam

  • @mahimasaha1569
    @mahimasaha1569 Год назад

    Thank you so much Ma’am for such informative videos which are not available elsewhere. You explained really well. Thank you so much. I would like to know much more about you and request you to share your LinkedIn profile or email id if possible to stay connected.

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      Thank you. My mail id is given in description box.

  • @shivkrupahalbandge6839
    @shivkrupahalbandge6839 Год назад

    Mam please do videos on techniques in rdt

  • @dales4625
    @dales4625 Год назад

    Cis acting vs upstream

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      SD sequence is cis acting and it is upstream sequence.

  • @LTG241
    @LTG241 Год назад

    Please make a video on (p)ppgpp metabolism

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      The video also explains about synthesis and degradation of ppGpp i. e. Metabolism.

  • @lubnayar8198
    @lubnayar8198 Год назад

    DIGE need more explanation..

  • @namithananditha3879
    @namithananditha3879 Год назад

    I didn't understand how the mismatching increases specificity ??

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      One of the biggest challenges for microarray-based approaches is to eliminate non-specific hybridization so that probe signals accurately reflect the presence and concentration of specific targets. For that purpose, Perfect match(PM) prob and mismatch(MM) prob are used. Actually Mismatch works as control. To have high accuracy and to avoid false positive result such PM/MM are used. It is specifically for homologous sequences like sequences from gene families. Target is allowed to hybridization with PM and cross hybrisize with MM prob. The signal intensity obtained from MM is subtracted from signal intensity of PM which would eliminate nonspecific signal and reveal actual level of specific hybridization i.e. concentration of real target . In this way mismatch prob help to analyze specificity.

    • @namithananditha3879
      @namithananditha3879 Год назад

      @@rohinipatil6606 Ohk tnq ! ☺️

  • @singwithme8860
    @singwithme8860 Год назад

    Ma'am this helped me a lot... Thank you so much ma'am<3

  • @michastanowski1093
    @michastanowski1093 Год назад

    Hello, great explanation! Thank you. What happens with broken mRNA though? And what is the role of SmpB protein in this process?

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      Thank you. Good question. RNaseR degrads broken RNA. SmpB interacts with tmRNA and delivers it to stalled ribosomes. C-terminal tail of SmpB is required for accommodation and traspeptidation .

  • @mindhiralekka4062
    @mindhiralekka4062 Год назад

    Mam I can't able to understand ,what is different between negative regulation and positive regulation, mam you taught that but get confused will you explain me with example.

    • @rohinipatil6606
      @rohinipatil6606 Год назад

      To clear your confusion watch video on lac operon, ara operon and trp operon which was uploded.

  • @akanshakamble7777
    @akanshakamble7777 Год назад

    I can clear my doubts through this lecture..thank you ma'am 🙏

  • @Yaya-xx6mp
    @Yaya-xx6mp Год назад

    Thank you from Algeria 🤍

  • @bantithakur400
    @bantithakur400 Год назад

    I watching 2 time and now averything is clear.... thank you mam

  • @rakshana7955
    @rakshana7955 Год назад

    Thank you ma'am, it was very helpful!

  • @kyraraines31
    @kyraraines31 Год назад

    Thank you so much for posting this!! This was such a clear explanation, helped me understand my classwork so much better <3 much thanks from a first year grad student :)

  • @nileshsharma0212
    @nileshsharma0212 Год назад

    Mam can you please make a detailed video on DIGE 🙏

  • @harshagautam5986
    @harshagautam5986 Год назад

    Very good explanation ma'am!

  • @gajananpatil3574
    @gajananpatil3574 Год назад

    खूप छान....

  • @Prashantkale11
    @Prashantkale11 Год назад

    Thank you

  • @maybolimarathiclasses-urmi6167

    Easy to understand ma'am 👍👍