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Please carefully examine the docked proteins after obtaining it form cluspro server. There might be some issue in the complex. Cloasley check for the merging errors using notepad++. Thanks

Thanks for subscribing to my channel. I already have made the tutorial relating your query. Watch PART-9 of this series, you will learn the installation of "mamba"... Secondly, I am not using Gatk4 for data analysis, so including that installation was not related to the series. Thanks

Thanks alot... Because these are the most abundant and beneficial normal flora having no such reported pathogenic potentials (might be...see literature)... Other bacteria may can be opted, until and unless you have a significant proves for their role effective and non pathogenic role in human gut...I mainly focused on the skill set and breif explanations...You are required to do critical thinking and extensive literature review before performing a step...Thanks

Brother, approch me via email, where we could discuss the queries and exchange the numbers...Thanks "nadeemkhanf577977@gmail.com"

I am glad that you are satified from my knowledge... I have aswered your query via mail...Thanks

Glad it helped...Thanks...stay tuned, you will see alot more coming...share it in your friend circle so that other could also get benefits...Thanks again...

Glad it helped...Thanks...stay tuned, you will see alot more coming...share it in your friend circle so that other could also get benefits...Thanks again...

Glad it helped...Thanks...stay tuned, you will see alot more coming...share it in your friend circle so that other could also get benefits...Thanks again...

Thank you so much 😀

I am also facing the same problem.

Did that issue get resolved?

@@kulsumfatima5633 No

The same issue arises when I was performing this analysis a couple of years ago but my superiors told me to keep trying by renaming the usearch file, move it to other folders, start again from the very start etc etc...And than it was resolved...I think this might be some issue with the algorithm or the files... I purely recommend to try some other tools that is based on linux or python...Dont rely on a single platform...Thanks

Yes, you can place your queries by mailing me at "nadeemkhanf577977@gmail.com" Thanks

I already had perform it...walk through the attached links...Thanks ruclips.net/video/C9W4GrWVbg4/видео.htmlsi=FDHcUOwl0UgvRaAH ruclips.net/video/1aHkmbSyiDY/видео.htmlsi=mYkJ927g5jovpuWM

The lower the cuttoff value the more the model will be confident over its answer, means this is the error value...you can set it by refering to different research articles or you can visit the actual paper of this specific tool. Setting parameters for different tools depend upon your problem statement, therefore you are supposed to visit the help of the tools and read its paper... Thanks

Well, the database explained in the video is purely used for the identification of virulence factors. You can also use PATRIC database.Thanks

Hi, Ajay...I already has started it from the very basics...explore the channel...Thanks ruclips.net/video/LA3zg4RuySI/видео.htmlsi=TqccJTZiKsHgbkmr

​@@scienceforeveryone8827Thanks brother, I have found it. ❤

use google colab for it...Thanks

noted...Will work on your instructions...Thanks

Almost similar...But please do a through literature review of your case study before proceeding...Thanks

Thanks for your appreciation... There are multiple probes that can demonstrate a similar gene...No issues with it...If you have same gene with 3 different expression values, than simple add all the expression values of divide the sum by total genes (take simple average of it) and consider the resultant new expression value. Thanks

⁠​⁠​⁠@@scienceforeveryone8827Thanks for your response. Averaging of gene expression value sounds interesting. I was wondering if we should consider taking average of all the significant multiple probeset expression value or should we filter out non-significant ones first? Also, are you aware of any function or package that can automate this process of averaging? Finally, if you could share some references supporting averaging of probesets would be really helpful. Appreciate your help!

@@scienceforeveryone8827 Never mind, I found a package and function to select the desirable probe sets. Thank you!

Extract all the DEGs symbols from the toptables (all three) and use TB-tools software to find the common genes (overlapping)...Thanks

@@scienceforeveryone8827 can you make a video on that plus also make video on Gene Ontology DAVID and KEGG

Most welcome!

yes, it have some limitations...

Because its present over the immune cells and also on normal cells and is highly specific to immune activations

Noted

Not sure about it, but it might be possible...just dig about it and do comment if debian can process this analysis...I will wait for your response...Thanks

hi, I just got your email...yes I am having a repo but havent uploaded the code so far...I will soon upload it...Thanks

@@scienceforeveryone8827 I missed the notif for this comment reply of yours. Actually, Im working on a project right now, so its kinda bit urgent. Would it be possible for you to provide me the script so I can compare it to my script to see the differences and get the best out of both scripts..? I'll be very grateful for this kindness of yours, Nadeem. Cheers, Saeed.